An easy long‐acting BMP7 release system based on biopolymer nanoparticles for inducing osteogenic differentiation of adipose mesenchymal stem cells

Chen, Rui; Yu, Jingjing; Gong, Hai‐Lun; Jiang, Yuquan; Xue, Mintao; Xu, Ning; Wei, Daixu; Shi, Changgui

doi:10.1002/term.3070

Cited by 24 publications

(25 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, with the introduction of BMP2, the cells capture efficiency of mADSCs on the polyester scaffold increased, which suggest that the loaded and released BMP2 molecules retained good bioactivity and were also able to assist the capture of cells dissociated in medium to PCL/PLGA-PEI. In previous reports [ 25 , 32 ], carriers composed of other polyester materials for the loading and release of BMP2, such as 2D films or 3D scaffolds/microspheres, were also found to promote the attachment of stem cells on surface, similar to our results.…”

Section: Resultssupporting

confidence: 92%

“…2 c). Similar to our previous reports [ 25 , 32 ], the properties of polyester microspheres and nanoparticles was also not changed by complexes of SL and BMP2, including size, surface charge and microstructure.…”

Section: Resultssupporting

confidence: 90%

“…In our earlier reports [ 25 , 32 ], we developed a physical mixture method for soybean lecithin-mediated introduction of growth factors into polyesters (SMIGP method), in which hydrophilic BMP2 was firstly modified by blending with the biosurfactant soybean lecithin (SL) to form hydrophobic SL-BMP2 complexes, named SBMP. Then solid-liquid phase separation was used to prepare the bioactive osteo-polyester scaffold (BOPSC) of PCL and PLGA-PEI with SBMP for in situ cell-capture and osteogenic induction of stem cells ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In addition to calcium ions [ 7 ], bioactive osteogenic growth factors also offer an appropriate microenvironment for the adhesion and capture of stem cells or osteoblasts, leading to osteogenic differentiation both in vitro or in vivo [ [29] , [30] , [31] , [32] ]. Bone morphogenetic protein 2 (BMP2) is a typical protein-based growth factor, which is essential for osteogenic differentiation of stem cells or osteogenic precursor cells.…”

Section: Introductionmentioning

confidence: 99%

“…Phospholipids, main components of cell membranes, which often play a role of intermediary between the water phase and the oil phase (organic phase) for applications in drug delivery field [ 25 , 32 ]. Due to the disadvantages of easy degradation, high cost and poor dispersion in polyesters, the use of untreated BMP2 in combination with polyesters was only reported in a limited number of studies [ 25 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

3D bioactive cell-free-scaffolds for in-vitro/in-vivo capture and directed osteoinduction of stem cells for bone tissue regeneration

Rahman

Peng

Zhao

et al. 2021

Bioactive Materials

View full text Add to dashboard Cite

Hydrophilic bone morphogenetic protein 2 (BMP2) is easily degraded and difficult to load onto hydrophobic carrier materials, which limits the application of polyester materials in bone tissue engineering. Based on soybean-lecithin as an adjuvant biosurfactant, we designed a novel cell-free-scaffold of polymer of poly(ε-caprolactone) and poly(lactide-co-glycolide)-co-polyetherimide with abundant entrapped and continuously released BMP2 for in vivo stem cell-capture and in situ osteogenic induction, avoiding the use of exogenous cells. The optimized bioactive osteo-polyester scaffold (BOPSC), i.e. SBMP-10SC, had a high BMP2 entrapment efficiency of 95.35%. Due to its higher porosity of 83.42%, higher water uptake ratio of 850%, and sustained BMP2 release with polymer degradation, BOPSCs were demonstrated to support excellent in vitro capture, proliferation, migration and osteogenic differentiation of mouse adipose derived mesenchymal stem cells (mADSCs), and performed much better than traditional BMP-10SCs with unmodified BMP2 and single polyester scaffolds (10SCs). Furthermore, in vivo capture and migration of stem cells and differentiation into osteoblasts was observed in mice implanted with BOPSCs without exogenous cells, which enabled allogeneic bone formation with a high bone mineral density and ratios of new bone volume to existing tissue volume after 6 months. The BOPSC is an advanced 3D cell-free platform with sustained BMP2 supply for in situ stem cell capture and osteoinduction in bone tissue engineering with potential for clinical translation.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

3D bioactive cell-free-scaffolds for in-vitro/in-vivo capture and directed osteoinduction of stem cells for bone tissue regeneration

Rahman

Peng

Zhao

et al. 2021

Bioactive Materials

View full text Add to dashboard Cite

show abstract

Osteogenic Induction and Anti‐Inflammatory Effects of Calcium‐Chlorogenic Acid Nanoparticles Remodel the Osteoimmunology Microenvironment for Accelerating Bone Repair

Liu,

Zhang,

Shi

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Successful bone regeneration requires the close cooperation between bone marrow mesenchymal stem cells (BMSCs) and macrophages, but the low osteogenic differentiation efficiency of stem cells and the excessive inflammatory response of immune cells hinder the development of the bone repair. It is necessary to develop a strategy that simultaneously regulates the osteogenic differentiation of BMSCs and the anti‐inflammatory polarization of macrophages for accelerating the bone regeneration. Herein, calcium‐chlorogenic acid nanoparticles (Ca‐CGA NPs) were synthesized by combining the small molecular of chlorogenic acid (CGA) with Ca2+. Ca‐CGA NPs internalized by cells could be dissolved to release free CGA and Ca2+ under low pH conditions in lysosomes. In vitro results demonstrated that Ca‐CGA NPs could not only enhance the osteogenic differentiation of BMSCs, but also promote the phenotype transformation of macrophages from M1 to M2. Furthermore, in vivo experiments confirmed that Ca‐CGA NPs treatment facilitated the recovery of rat skull defect model through both the osteoinduction and immunomodulation. This study develops a new Ca‐CGA NPs‐based strategy to induce the differentiation of BMSCs into osteoblasts and the polarization of macrophages into M2 phenotype, which is promising for accelerating bone repair.This article is protected by copyright. All rights reserved

show abstract

3‐hydroxybutyrate in the brain: Biosynthesis, function, and disease therapy

Wang

Xiao

et al. 2023

Brain-X

Self Cite

View full text Add to dashboard Cite

3-hydroxybutyrate (3HB), or BHB, is an anionic small molecule acid metabolite with a hydroxyl group. 3HB is the major ketone body that is distributed in the human brain and its primary energy source when glucose is absent. A better understanding of 3HB and how to adapt neuronal response mechanisms is expected to facilitate the development of new interventions to promote cognitive brain function and prevent neurodegenerative diseases. It provides important concepts for the clinical application of 3HB therapy. This review summarizes the distribution of 3HB in the brain, its properties, and its mechanism in brain and nerve regulation. We focus on 3HB biosynthesis in natural human cells and engineered bacteria via synthetic biology platforms and 3HB treatment in various brain and nerve diseases, including epilepsy, multiple sclerosis, stroke, Parkinson's disease, Alzheimer's disease, Huntington's disease, depressive disorder, and schizophrenia. Ultimately, this review explores the future development trend of 3HB as a potential smallmolecule drug for brain and nerve diseases. K E Y W O R D S3-hydroxybutyrate, ketogenic diet, ketone body, polyhydroxyalkanoates, synthetic biology | INTRODUCTION3-hydroxybutyrate (3HB), or BHB, is an important ketone body (KB) mainly distributed in the liver, muscle, and brain. [1] Under prolonged fasting and starvation conditions, KBs meet up to ~60% of the brain's energy requirements. [2] 3HB is mainly synthesized in the liver via fatty acid oxidation. [3] It is present in the adipose tissue and human milk and is required for converting ketogenic amino acids. [4] In vitro studies have shown that 3HB can be synthesized endogenously in astrocytes, the only cell type in the brain that is able to oxidize fatty acids. [5] However, how 3HB is metabolized in the brain using free fatty acids remains unknown. Besides providing energy, 3HB also plays an important role in several signaling pathways, such as inflammation, oxidative stress response, K +adenosine triphosphate (K + -ATP) channels, Ca 2+ channels, histone acetylation, and G protein-coupled receptors.In addition, 3HB is the primary degradation product of novel biomaterial polyhydroxyalkanoates (PHAs). [6] Compared to small acidic polylactic acid [7,8] and polycaprolactone molecules, [9,10] the moderate acidity [11] and minimal tissue rejection [12,13] of 3HB slowly released from PHAs [14] can Bing-Long Wang and Jian-Fei Wu are equal contributors.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

show abstract

An easy long‐acting BMP7 release system based on biopolymer nanoparticles for inducing osteogenic differentiation of adipose mesenchymal stem cells

Cited by 24 publications

References 28 publications

3D bioactive cell-free-scaffolds for in-vitro/in-vivo capture and directed osteoinduction of stem cells for bone tissue regeneration

3D bioactive cell-free-scaffolds for in-vitro/in-vivo capture and directed osteoinduction of stem cells for bone tissue regeneration

Osteogenic Induction and Anti‐Inflammatory Effects of Calcium‐Chlorogenic Acid Nanoparticles Remodel the Osteoimmunology Microenvironment for Accelerating Bone Repair

3‐hydroxybutyrate in the brain: Biosynthesis, function, and disease therapy

Contact Info

Product

Resources

About