<scp>PCL</scp>‐<scp>PEG</scp>‐<scp>PCL</scp> film promotes cartilage regeneration in vivo

Fu, Na; Liao, Jinfeng; Lin, Shiyu; Sun, Kai; Tian, Taoran; Zhu, Bofeng; Lin, Yunfeng

doi:10.1111/cpr.12295

Cited by 50 publications

(37 citation statements)

References 53 publications

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“…PCL has been approved by the US Food and Drug Administration for internal use in the human body with remarkable mechanical properties and perfect biocompatibility. Thus, it is commonly used as a scaffold‐reinforcing material to improve mechanical properties 26. Evidently, the combination of DWJM and PCL is an efficient approach to overcome each material's respective shortcomings.…”

Section: Discussionmentioning

confidence: 99%

Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

Duan

et al. 2020

Adv Funct Materials

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Wharton's jelly (WJ) is considered a potential scaffold in tissue‐engineered trachea for its similar composition and function to cartilage tissue. However, the feasibility of using WJ to construct engineered neocartilage tissue has not been reported, let alone tubular tracheal cartilage regeneration and segmental tracheal lesion repair. Here, electrospun nanofibrous membranes composed of three different decellularized WJ matrix (DWJM)/poly(ε‐caprolactone) (PCL) ratios (8:2, 5:5, and 2:8) are fabricated. The results demonstrate improved degradation speed, absorption, and cell adhesion capacity but weakened mechanical properties with increased DWJM content, but satisfactory homogeneous cartilage regeneration is only achieved in the DWJM/PCL (8:2) group after 12 weeks in vivo culture. Furthermore, homogeneous, 3D, tubular, trachea‐shaped cartilage is constructed with a controllable lumen diameter and wall thickness based on the 2D nanofibrous membrane using a modified sandwich model, in which the chondrocyte‐membrane construct is rolled around a silicon tube. Most importantly, by combining the above schemes with previously established vascularization and epithelialization techniques, chondrification, vascularization, and epithelialization are achieved simultaneously thus realizing long‐term (6 months) circumferential tracheal lesion repair in a rabbit model with a biological structure and function similar to that of native trachea, representing a promising approach for the clinical application of tracheal tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

Duan

et al. 2020

Adv Funct Materials

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“…For observation of cell attachment and morphological changes, A549 cells were plated on varying stiffness of PDMS substrates at 1 × 10 4 cells per well. A549 cells were fixed by 2.5% glutaraldehyde for 2 hr at 4°C and then dehydrated in a graded ethanol series (30%, 50%, 75%, 85%, 95%, and 100%) for 15 min at each step, as described previously (Fu et al, ). After being dried in an exhaust hood, the specimens were mounted on specimen holders, coated with a thin layer of gold, and then examined by scanning electron microscope (SEM).…”

Section: Methodsmentioning

confidence: 99%

Substrate stiffness regulated migration and angiogenesis potential of A549 cells and HUVECs

Zhao

Xue

et al. 2017

Journal Cellular Physiology

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Tumor tissue tends to stiffen during solid tumor progression. Substrate stiffness is known to alter cell behaviors, such as proliferation and migration, during which angiogenesis is requisite. Mono- and co-culture systems of lung cancer cell line A549 and human umbilical vein endothelial cells (HUVECs), on polydimethylsiloxane substrates (PDMS) with varying stiffness, were used for investigating the effects of substrate stiffness on the migration and angiogenesis of lung cancer. The expressions of matrix metalloproteinases (MMPs) and angiogenesis-related growth factors were up-regulated with the increase of substrate stiffness, whereas that of tissue inhibitor of matrix metalloproteinase (TIMPs) were down-regulated with increasing substrate stiffness. Our data not only suggested that stiff substrate may promote the migration and angiogenesis capacities of lung cancer, but also suggested that therapeutically targeting lung tumor stiffness or response of ECs to lung tumor stiffness may help reduce migration and angiogenesis of lung tumor.

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“…Polydimethylsiloxane (PDMS) matrices, serve as two‐dimensional culture formats, and can be cured using an organometallic crosslinking reaction. When different proportions of base oligomer and curing agent are used, the fully cross‐linked matrices produced display a wide range of stiffness, reflective of those found within natural tissues, including osteoid, cartilage, and adipose tissue (Fu et al, ; Liao et al, ; Shi et al, ). Given this flexibility, PDMS‐based matrices were used in this study.…”

Section: Introductionmentioning

confidence: 99%

Regulating osteogenesis and adipogenesis in adipose‐derived stem cells by controlling underlying substrate stiffness

Zhang

Lin

Shao

et al. 2017

Journal Cellular Physiology

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Cells reside in a complex microenvironment (niche) in which the biochemical and biophysical properties of the extracellular matrix profoundly affect cell behavior. Extracellular stiffness, one important bio-mechanical characteristic of the cell niche, is important in regulating cell proliferation, migration, and lineage specification. However, the mechanism by which mechanical signals guide osteogenic and adipogenic commitment of stem cells remains difficult to dissect. To explore this question, we generated a range of polydimethylsiloxane-based matrices with differing degrees of stiffness that mimicked the stiffness seen in natural tissues and examined adipose stem cell morphology, spreading, vinculin expression, and differentiation along the osteogenic and adipogenic pathways. Rigid matrices allowed broader cell spreading, faster growth rate and stronger expression of vinculin in adipose-derived stem cells. In the presence of inductive culture media, stiffness-dependent osteogenesis and adipogenesis of the adipose stem cells indicated that there was a combinatorial effect of biophysical and biochemical cues; no such lineage specification was observed in normal media. Osteogenic differentiation behavior showed a correlation with matrix rigidity, as well as with elevated expression of RhoA, ROCK-1/-2, and related proteins in the Wnt/β-catenin pathway. The result provides a comprehensive understanding of how stem cells respond to the surrounding microenvironment and points to the fact that matrix stiffness is a critical element in biomaterial design and this will be an important advance in stem cell-based tissue engineering.

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PCL‐PEG‐PCL film promotes cartilage regeneration in vivo

Abstract: These results demonstrated that PCEC film was a good scaffold for cartilage tissue engineering for improving cell proliferation and adhesion and could lead to excellent repair of cartilage defects.

Cited by 50 publications

References 53 publications

Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

Substrate stiffness regulated migration and angiogenesis potential of A549 cells and HUVECs

Regulating osteogenesis and adipogenesis in adipose‐derived stem cells by controlling underlying substrate stiffness

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