P1.1.16 Development of Amperometric Biosensor Based on Tyrosinase Immobilized in Phosphate-Doped Polypyrrole Film for Detection of Biogenic Amines

Apetrei, Irina Mirela; Tutunaru, Dana; Popa, C. V.; Apetrei, Constantin

doi:10.5162/imcs2012/p1.1.16

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…In the previous reports, only two pure graphene-based modied electrodes (shown in Table 1) were investigated as the sensor for EP. 42,44 The LOD for EP in this work is higher than that at the graphene-modied GCE, 42 almost the same as that at the graphene-modied screen-printed electrode. 44 It should be specially stated that the graphene-modied GCE sensor was used to detect EP with the electrolyte of pH 4.0, 42 different from pH 7.0 used here.…”

Section: Electrochemical Detection Of Epinephrine and Metanephrinementioning

confidence: 51%

“…42,44 The LOD for EP in this work is higher than that at the graphene-modied GCE, 42 almost the same as that at the graphene-modied screen-printed electrode. 44 It should be specially stated that the graphene-modied GCE sensor was used to detect EP with the electrolyte of pH 4.0, 42 different from pH 7.0 used here. The width of the linear range for EP detection in this work is three orders of magnitude, the same as that at the graphene-modied GCE, 42 larger than that at the graphene-modied screen-printed electrode.…”

Section: Electrochemical Detection Of Epinephrine and Metanephrinementioning

confidence: 51%

“…The width of the linear range for EP detection in this work is three orders of magnitude, the same as that at the graphene-modied GCE, 42 larger than that at the graphene-modied screen-printed electrode. 44 Generally, the NG/CS/GCE exhibits good sensing results for EP and MEP.…”

Section: Electrochemical Detection Of Epinephrine and Metanephrinementioning

confidence: 97%

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Detection of epinephrine and metanephrine at a nitrogen doped three-dimensional porous graphene modified electrode

Yang

Bai

et al. 2015

Anal. Methods

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Section: Electrochemical Detection Of Epinephrine and Metanephrinementioning

confidence: 51%

Section: Electrochemical Detection Of Epinephrine and Metanephrinementioning

confidence: 51%

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Detection of epinephrine and metanephrine at a nitrogen doped three-dimensional porous graphene modified electrode

Yang

Bai

et al. 2015

Anal. Methods

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Nanobiosensors for Detection of Phenolic Compounds

Achi

Bensana

Bouguettoucha

et al. 2020

Nanotechnology in the Life Sciences

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PVA-AWP/tyrosinase functionalized screen-printed electrodes for dopamine determination

et al. 2016

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The development of highly sensitive amperometric biosensors for dopamine determination using commercially available SPEs in combination with the PVA-AWP photopolymer as the enzyme immobilization matrix is described in this work. This research focuses on (i) the optimization of the conditions for SPE biofunctionalization by tyrosinase entrapment in PVA-AWP and of the working conditions for dopamine determination by mathematical model application, and (ii) the evaluation of the analytical performances of the PVA-AWP/tyrosinase functionalized SPEs. Dopamine determination under optimum conditions for electrode biofunctionalization (PVA-AWP 3%, 60 min UV light exposure) andunder optimum working conditions (pH 6.5, 25 C) was performed in the dynamic concentration range of 0.9-500 mmol L À1 , 0.2-400 mmol L À1 , and 0.03-150 mmol L À1 , using respectively C SPE, MWCNT-GNP/C SPE, and graphene-GNP/C SPE. The LOD was found to be 300 nmol L À1 , 60 nmol L À1 , and as low as 10 nmol L À1 , correspondingly. The excellent analytical performances of the graphene-GNP C SPE and MWCNT-GNP C SPE were associated with their large active area (19.72 mm 2 and 15.3 mm 2 ) and enhanced electrocatalytic properties (k o ¼ 8.1 Â 10 À3 cm s À1 and 4.4 Â 10 À3 cm s À1 ) compared with the bare C SPE (12.47 mm 2 and k o ¼ 3.0 Â 10 À3 cm s À1 ). The developed biosensors were stable, reproducible, and more sensitive than most of the known biosensors for dopamine determination. They were successfully applied for dopamine determination in injections. Taking into consideration the fact that the selected enzyme immobilization approach using a water-soluble photopolymer opens the possibility of SPE biofunctionalization by photolithography, the developed biosensors are promising for fast, simple, sensitive, selective, and cost effective analysis of dopamine.

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P1.1.16 Development of Amperometric Biosensor Based on Tyrosinase Immobilized in Phosphate-Doped Polypyrrole Film for Detection of Biogenic Amines

Cited by 4 publications

References 18 publications

Detection of epinephrine and metanephrine at a nitrogen doped three-dimensional porous graphene modified electrode

Detection of epinephrine and metanephrine at a nitrogen doped three-dimensional porous graphene modified electrode

Nanobiosensors for Detection of Phenolic Compounds

PVA-AWP/tyrosinase functionalized screen-printed electrodes for dopamine determination

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