Nanobiosensors for Detection of Phenolic Compounds

Achi, Fethi; Bensana, Amira; Bouguettoucha, Abdallah; Chebli, Derradji

doi:10.1007/978-3-030-45116-5_10

Cited by 5 publications

(1 citation statement)

References 123 publications

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“…The enzymatic biosensors were widely used because of their ability to detect a target substance/analyte with a high specificity through enzyme-catalyzed reactions [5][6][7]. The detection of phenolic [8,9] and other compounds that can be used as a food preservative, and can be hazardous to human health at levels greater than authorized safety standards, such as benzoic acid, sodium azide and kojic acid [10], is of great interest for both quality assurance and consumer protection [11]. Tyrosinase-based amperometric biosensors have been extensively reported in the literature for the detection of various compounds (particularly mono-and di-phenols) due to their rapid response, low cost and low energy consumption [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

The Kinetic and Analytical Aspects of Enzyme Competitive Inhibition: Sensing of Tyrosinase Inhibitors

Attaallah

Amine

2021

Biosensors

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An amperometric biosensor based on tyrosinase, immobilized onto a carbon black paste electrode using glutaraldehyde and BSA was constructed to detect competitive inhibitors. Three inhibitors were used in this study: benzoic acid, sodium azide, and kojic acid, and the obtained values for fifty percent of inhibition (IC50) were 119 µM, 1480 µM, and 30 µM, respectively. The type of inhibition can also be determined from the curve of the degree of inhibition by considering the shift of the inhibition curves. Amperometric experiments were performed with a biosensor polarized at the potential −0.15 V vs. Ag/AgCl and using 0.1 M phosphate buffer (pH 6.8) as an electrolyte. Under optimized conditions, the proposed biosensor showed a linear amperometric response toward catechol detection from 0.5 µM to 38 µM with a detection limit of 0.35 µM (S/N = 3), and its sensitivity was 66.5 mA M−1 cm−2. Moreover, the biosensor exhibited a good storage stability. Conversely, a novel graphical plot for the determination of reversible competitive inhibition was represented for free tyrosinase. The graph consisted of plotting the half-time reaction (t1/2) as a function of the inhibitor concentration at various substrate concentrations. This innovative method relevance was demonstrated in the case of kojic acid using a colorimetric bioassay relying on tyrosinase inhibition. The results showed that the t1/2 provides an extended linear range of tyrosinase inhibitors.

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Section: Introductionmentioning

confidence: 99%

The Kinetic and Analytical Aspects of Enzyme Competitive Inhibition: Sensing of Tyrosinase Inhibitors

Attaallah

Amine

2021

Biosensors

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Disposable and Flexible Electrochemical Paper‐based Analytical Devices Using Low‐cost Conductive Ink

et al. 2021

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In this work, a simple procedure for construction of disposable electrochemical paper‐based analytical devices (ePADs) by screen‐printing using low‐cost materials and a home craft electronic printer is proposed. The devices were constructed using liner paper as a substrate and carbon ink prepared with graphite powder and wood glue. The ePAD was evaluated as an electrochemical sensor and biosensor. The proposed conductive carbon‐based ink can be easily prepared and is an eco‐friendly and non‐toxic material. The developed ePAD was simple to produce and can be used as a low‐cost electrochemical sensor, at less than US $0.20 per device.

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