Ozone Treatment of Alveolar Bone in the Cape Chacma Baboon Does Not Enhance Healing Following Trauma

Abstract

In the international literature, the role of Ozone (O3) in the advancement in alveolar bone healing in the absence of bone pathology was not tested before. The purpose of this study was to evaluate alveolar bone regeneration after a bone defect was created and treated with a single topical administration of O3. Alveolar bone defects were created on five healthy chacma baboons. One side of the maxilla and mandible was topically treated with a single treatment of an O3/O2 mixture (3,5-4 % O3), while the opposite sides were not treated and thus served as control. Regeneration was measured radiologically, using a standardized gray scale, as the increase in bone density in the treatment area at 3 and 6 weeks post-operative and was statistically analyzed using multivariate analysis of variance (MANOVA). There were no significant differences in densities observed between the O3/O2 mixture treatment and the control (p > 0.05). A single O3 treatment did not increase alveolar bone healing over a 3- and 6-week period in the mandible and the maxilla.

Effect of Ozonated Olive Oil in Topical Application towards Osteoblast Number and Angiogenesis of Alveolar Bone in Periodontitis Healing Process (in vivo study in Sprague dawley Rats)

“…The decrease is due to the mediator of angiogenesis, namely VEGF, began to decline in number after 11 days (Uchida et al, 2003). In the final stage of healing, the angiogenesis process was pressed, the number of growth factors decreased, and the inflammatory process subsided (Kotze et al, 2014). The blood vessels will start to decrease when nutritional needs in tissues were fulfilled (Kumar et al, 2007).…”

“…Oxygen is carried by the blood vessels so that with increasing oxygenation in the injured tissue area, the number of blood vessels will also increase. Oxygenation improved by the application of ozonated olive oil will neutralize the damaging anoxic phase of bone tissue trauma so that tissue healing will go smoothly (Kotze et al, 2014).…”

“…In the bone healing process, angiogenesis serves as an essential process in improving blood supply that will trigger the regeneration of bone tissue (Kotze et al, 2014). Growth factor that plays a role in the angiogenesis process includes Fibroblast Growth Factor (FGF), Vascular Endothelial Growth Factor (VEGF), and Platelet-Derived Growth Factor (PDGF).…”

A comparison of mandibular and maxillary alveolar osteogenesis over six weeks: a radiological examination

“…The process of bone regeneration can be monitored radiologically as bone density becomes more detectable during the regeneration process [ 1 , 17 , 18 ]. A significant correlation between mineral bone density and bone structure exists when density is measured radiologically [ 19 ].…”

Ozon uygulaması ve oral cerrahideki yeri: Derleme

“…etkiyi topikal ozon uygulamasının yapamadığı bulunmuştur.Antiseptik ajan olarak kullanılan ozonlu suyun gaz formundaki ozon ve diğer antiseptik ajanlara (% 2 , %0,2 klorheksidin diglukonat ; %5,5 , %2,25 sodyum hipoklorit ; %3 hidrojen peroksit ) göre sitotoksisitesinin değerlendirildiği bir başka çalışmada ise ozonlu suyun diğerlerine göre daha az sitotoksisite gösterdiği bulunmuştur. Bu nedenle ozonun sulu formu ağız içi uygulamalar için biyouyumluluk açısından optimal düzeyde hücrelerin biyolojik özelliklerini korur[1,3,9,[14][15][16].Ozon ile diğer tedavi yönteminde hastadan alınan kan ozonlanılıp aynı hastaya tekrardan geri verilir. Ozonlanan kan intravenöz (İV) yolla yada kas içi enjeksiyon yoluyla uygulanabilir.Ozonun parenteral uygulamaları ; intravenöz, intraarteriyel , subkütanöz, intraperitoneal, intraplevral, intraartiküler, intradiskal, intraforaminal, intralezyonel , topikal yada lokorejyonal uygulamalar , nazal, tübal, aurikülar, oral, vajinal, üretral veya mesane içi, rektal , kütanöz , dental uygulamalar gibi 30 farklı uygulama vardır[5,7,8].Ozonun intravenöz uygulanmasından biri majör otohematerapidir.…”