Oxytetracycline on functions and structure of soil microbial community

Chen, Wei; Liu, W; Pan, Neng; Jiao, Wentao; Wang, M

doi:10.4067/s0718-95162013005000076

Cited by 25 publications

(30 citation statements)

References 22 publications

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“…The influence of VAs on total bacteria and total fungi PLFA markers and their ratio appears to be dependent on a number of variables, including the soil, VA, and manure properties and concentrations. Chen et al (2013) reported increased bacteria and fungi PLFA markers in loam soil at OTC concentrations ≤15 mg kg -1 . However, at OTC concentrations >15 mg kg -1 , fungi were adversely affected to a greater extent than bacteria, and Gram-positive bacteria were reduced to a greater extent than Gram-negative bacteria.…”

Section: Phospholipid Fatty Acidsmentioning

confidence: 94%

Veterinary Antibiotic Effects on Atrazine Degradation and Soil Microorganisms

et al. 2016

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Veterinary antibiotics (VAs) in manure applied to agricultural lands may change agrichemical degradation by altering soil microbial community structure or function. The objectives of this study were to investigate the influence of two VAs, sulfamethazine (SMZ) and oxytetracycline (OTC), on atrazine (ATZ) degradation, soil microbial enzymatic activity, and phospholipid fatty acid (PLFA) markers. Sandy loam soil with and without 5% swine manure (w/w) was amended with 0 or 500 μg kgC radiolabeled ATZ and with 0, 100, or 1000 μg kg SMZ or OTC and incubated at 25°C in the dark for 96 d. The half-life of ATZ was not significantly affected by VA treatment in the presence or absence of manure; however, the VAs significantly ( < 0.05) inhibited ATZ mineralization in soil without manure (25-50% reduction). Manure amendment decreased ATZ degradation by 22%, reduced ATZ mineralization by 50%, and increased the half-life of ATZ by >10 d. The VAs had limited adverse effects on the microbial enzymes β-glucosidase and dehydrogenase in soils with and without manure. In contrast, manure application stimulated dehydrogenase activity and altered chlorinated ATZ metabolite profiles. Concentrations of PLFA markers were reduced by additions of ATZ, manure, OTC, and SMZ; adverse additive effects of combined treatments were noted for arbuscular mycorrhizal fungi and actinobacteria. In this work, the VAs did not influence persistence of the ATZ parent compound or chlorinated ATZ metabolite formation and degradation. However, reduced CO evolved from VA-treated soil suggests an inhibition to the degradation of other ATZ metabolites due to an altered soil microbial community structure.

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Section: Phospholipid Fatty Acidsmentioning

confidence: 94%

Veterinary Antibiotic Effects on Atrazine Degradation and Soil Microorganisms

et al. 2016

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“…Many studies have revealed that antibiotics affect the number of different groups of microorganisms (Pinna et al, 2012 ; Akimenko et al, 2015 ; Xu et al, 2016 ), the structural and genetic diversity of microorganisms and the overall microbial activity (Demoling et al, 2009 ; Cui et al, 2013 ; Liu et al, 2014 ; Reichel et al, 2014a , b ; Cycoń et al, 2016a ; Xu et al, 2016 ). Moreover, the impact of antibiotics on the enzyme activities, carbon mineralization, and nitrogen cycling has been proven (Liu et al, 2009 ; Kotzerke et al, 2011 ; Rosendahl et al, 2012 ; Chen et al, 2013 ; Ma et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Vancomycin and/or Multidrug-Resistant Citrobacter Freundii Altered the Metabolic Pattern of Soil Microbial Community

et al. 2018

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Despite many studies, our knowledge on the impact of antibiotics and antibiotic-resistant bacteria on the metabolic activity of soil microbial communities is still limited. To ascertain this impact, the community level physiological profiles (CLPPs) and the activity of selected enzymes (dehydrogenase, urease, and phosphatases) in soils treated with vancomycin (VA) and/or multidrug resistant Citrobacter freundii were determined during a 90-day experiment. A multivariate analysis and the resistance (RS)/resilience (RL) concept were used to assess the potential of native microorganisms to maintain their catabolic activity under exposure of VA and/or a high level of C. freundii. In addition, the dissipation rate of VA was evaluated in non-sterile (nsS) and sterile (sS) soils. The results revealed a negative impact of VA on the metabolic activity of soil microorganisms on days 1, 15, and 30 as was showed by a decrease in the values of the CLPP indices (10–69%) and the enzyme activities (6–32%) for treated soils as compared to the control. These observations suggested a low initial resistance of soil microorganisms to VA and/or C. freundii but they were resilient in the long term. Considering the mean values of the RS index, the resistance of measured parameters was categorized in the following order: alkaline phosphatase (0.919) > acid phosphatase (0.899) > dehydrogenase (0.853) > the evenness index (0.840) > urease (0.833) > the Shannon-Wiener index (0.735) > substrate richness (0.485) > the AWCD (0.301). The dissipation process of VA was relatively fast and independent of the concentration used. The DT50 values for VA applied at both concentrations were about 16 days. In addition, the dissipation of VA in nsS was three times faster compared to the dissipation of antibiotic in sS. In conclusion, both CLPP and enzyme activities assays appeared to be useful tool for the determination of disturbances within soil microbial communities and used together may be helpful to understand the changes in their catabolic features. The entry of large quantities of VA and/or C. freundii into soil may temporarily change microbial activity thus pose a potential risk for soil functioning.

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“…The initial concentration of OTC added to the soil at 150 mg kg −1 was selected on the basis of a literature search. The other authors used 1 to 300 mg kg −1 of tetracycline in their studies (Chen et al 2013;Molaei et al 2017;Duan et al 2017). During the 30-day incubation of soil contaminated with antibiotic, the concentration of OTC content was measured by liquid chromatography.…”

Section: Resultsmentioning

confidence: 99%

Veterinary antibiotic oxytetracycline’s effect on the soil microbial community

2020

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Background: Antibiotics are widely used to treat animals from infections. After fertilizing, antibacterials can remain in the soil while adversely affecting the soil microorganisms. The concentration of oxytetracycline (OTC) in the soil and its effect on the soil microbial community was assessed. To assess the impact of OTC on the soil microbial community, it was added to the soil at concentrations of 50, 150, and 300 mg kg-1 and incubated for 35 days. Results: The concentration of OTC added to the soil decreased from 150 to 7.6 mg kg-1 during 30 days of incubation, as revealed by LC-MS. The deviations from the control values in the level of substrate-induced respiration on the 5th day of the experiment were, on average, 26, 68, and 90%, with OTC concentrations at 50, 150, and 300 mg kg-1 , respectively. In samples with 150 and 300 mg kg-1 of OTC, the number of bacteria from the 3rd to 14th day was 2-3 orders of magnitude lower than in the control. The addition of OTC did not affect the fungal counts in samples except on the 7th and 14th days for the 150 and 300 mg kg-1 contaminated samples. Genes tet(M) and tet(X) were found in samples containing 50, 150, and 300 mg kg-1 OTC, with no significant differences in the number of copies of tet(M) and tet(X) genes from the OTC concentration. Conclusions: Our results showed that even after a decrease in antibiotic availability, its influence on the soil microbial community remains.

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Oxytetracycline on functions and structure of soil microbial community

Cited by 25 publications

References 22 publications

Veterinary Antibiotic Effects on Atrazine Degradation and Soil Microorganisms

Veterinary Antibiotic Effects on Atrazine Degradation and Soil Microorganisms

Vancomycin and/or Multidrug-Resistant Citrobacter Freundii Altered the Metabolic Pattern of Soil Microbial Community

Veterinary antibiotic oxytetracycline’s effect on the soil microbial community

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