Oxymatrine Prevents NF-κB Nuclear Translocation And Ameliorates Acute Intestinal Inflammation

Abstract

Oxymatrine is a traditional Chinese herbal product that exhibits anti-inflammatory effects in models of heart, brain and liver injury. We investigated the impact of oxymatrine in an acute model of intestinal injury and inflammation. Oxymatrine significantly decreased LPS-induced NF-κB-driven luciferase activity, correlating with diminished induction of Cxcl2, Tnfα and Il6 mRNA expression in rat IEC-6 and murine BMDC. Although oxymatrine decreased LPS-induced p65 nuclear translocation and binding to the Cxcl2 gene promoter, this effect was independent of IκBα degradation/phosphorylation. DSS-induced weight loss and histological damage were ameliorated in oxymatrine-treated C57BL/6-WT-mice. While this effect correlated with reduced colonic Il6 and Il1β mRNA accumulation, global NF-κB activity as measured in NF-κBEGFP mice was unaffected. Our data demonstrate that oxymatrine reduces LPS-induced NF-κB nuclear translocation and activity independently of IκBα status, prevents intestinal inflammation through blockade of inflammatory signaling and ameliorates overall intestinal inflammation in vivo.

Benzo(a)pyrene induces oxidative stress, pro-inflammatory cytokines, expression of nuclear factor-kappa B and deregulation of wnt/beta-catenin signaling in colons of BALB/c mice

“…Activation of NF-κB with concomitant over expression of TNF-α and IL-1β in acute colon inflammation in animal studies and intestinal specimens from patients with crohn's disease has been previously reported (Franchimont et al, 2004;Guzman et al, 2013).…”

Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells

“…OMT has been reported to have various medicinal qualities, including antiviral [7], antifibrotic [8], anti-inflammatory [9], and antiarrhythmic [10] effects, and is also widely used in China for the treatment of chronic hepatitis B. Previous in vitro and in vivo studies have shown that OMT inhibits cell proliferation and induces apoptosis in various types of cancers [11–15].…”

RETRACTED: Effects of Oxymatrine on the Proliferation and Apoptosis of Human Hepatoma Carcinoma Cells

“…Oxymatrine (molecular formula, C 15 H 24 N 2 O 2 ; see Figure 1) is one of the key components extracted from Sophora flavescens, a leguminous plant grown in China, Japan, and some European countries, 10 and has attracted much attention because of its low toxicity and side effects. Extensive research over the past decades have revealed various important pharmacological activities of oxymatrine under in vitro and in vivo conditions, including anti-inflammation, immunoregulatory, antihepatitis virus infection, antihepatic fibrosis, antianaphylaxis, and other immune regulation.…”