Benzo(a)pyrene induces oxidative stress, pro-inflammatory cytokines, expression of nuclear factor-kappa B and deregulation of wnt/beta-catenin signaling in colons of BALB/c mice

Ajayi, Babajide O.; Adedara, Isaac A.; Farombi, Ebenezer O.

doi:10.1016/j.fct.2016.06.019

Cited by 59 publications

(30 citation statements)

References 65 publications

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“…It was observed that colon epithelial cells treated with BaP had lower SOD activity and GSH levels as compared to the untreated controls (see Figure 2). Our results are supported by previous reports [23,26], which have shown that GSH and SOD are overutilized during the increased demand for detoxification of free radicals caused by the presence of BaP [24]. Moreover, BaP not only affected protein expression of the colon epithelial cells to a relatively high extent but also damaged their DNA and mRNA [26].…”

Section: Discussionsupporting

confidence: 91%

“…To evaluate the potential of the inactivated bifidobacterial strain in relieving BaP damage, 50 µM BaP was selected as an inhibition concentration for the following tests. Partially, oxidative stress has been regarded to be responsible for the cytotoxicity following BaP exposure, as BaP causes cellular oxidative damage by increasing the levels of free radicals in the cells [23,24]. The intracellular redox status is tightly regulated by the presence of enzymatic and non-enzymatic antioxidants, which play important roles in maintaining the free radical balance in the organisms [25].…”

Section: Discussionmentioning

confidence: 99%

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Potential of Inactivated Bifidobacterium Strain in Attenuating Benzo(A)Pyrene Exposure-Induced Damage in Colon Epithelial Cells In Vitro

Zhang

et al. 2020

Toxics

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Long-term exposure to benzo(a)pyrene (BaP) poses a serious genotoxic threat to human beings. This in vitro study investigated the potential of inactivated Bifidobacterium animalis subsp. lactis BI-04 in alleviating the damage caused by BaP in colon epithelial cells. A concentration of BaP higher than 50 μM strongly inhibited the growth of colon epithelial cells. The colon epithelial cells were treated with 50 μM BaP in the presence or absence of inactivated strain BI-04 (~5 × 108 CFU/mL). The BaP-induced apoptosis of the colon epithelial cells was retarded in the presence of B. lactis BI-04 through activation of the PI3K/ AKT signaling pathway, and p53 gene expression was decreased. The presence of the BI-04 strain reduced the intracellular oxidative stress and DNA damage incurred in the colon epithelial cells by BaP treatment due to the enhanced expression of antioxidant enzymes and metabolism-related enzymes (CYP1A1). The data from comet assay, qRT-PCR, and western blot analysis showed that the cytotoxic effects of BaP on colon epithelial cells were largely alleviated because the bifidobacterial strain could bind to this carcinogenic compound. The in vitro study highlights that the consumption of commercial probiotic strain BI-04 might be a promising strategy to mitigate BaP cytotoxicity.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Potential of Inactivated Bifidobacterium Strain in Attenuating Benzo(A)Pyrene Exposure-Induced Damage in Colon Epithelial Cells In Vitro

Zhang

et al. 2020

Toxics

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“…However, persistent and excessive autophagy leads to cell death (Swart et al, 2016). Previous studies unraveled that BaP promotes the release of pro-inflammatory cytokines and increases the protein expression of iNOS in mice colon (Ajayi et al, 2016), lungs (Shahid et al, 2016;Shahid et al, 2017), airway (Yangisawa et al, 2016), and intestine (Ribiere et al, 2016) and zebrafish embryos (Duan et al, 2016). That is to say, BaP triggers inflammation.…”

Section: Introductionmentioning

confidence: 98%

Benzo[a]pyrene induces pyroptotic and autophagic death through inhibiting PI3K/Akt signaling pathway in HL-7702 human normal liver cells

Gao

Deng

et al. 2019

J. Toxicol. Sci.

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-Benzo(α)pyrene (BaP) possesses a forceful hepatotoxicity, and is ubiquitous in foods and ambient air. Our previous study found that BaP induced pyroptotic and autophagic death in HL-7702 human liver cells; the relevant mechanisms, however, remain unknown. This work was therefore to unravel the effects of the PI3K/Akt signaling pathway on pyroptotic and autophagic death triggered by BaP. Cells were treated with or without LY294002 (PI3K/Akt inhibitor) and IGF-1 (PI3K/Akt activator) before BaP exposure, and the results showed that compared with the control, the protein expression of p-Akt was markedly decreased by BaP (p < 0.05). IGF-1 did not subvert this inhibitive effect of BaP, while LY294002 enhanced it. Furthermore, the protein expression of pyroptosis (Cleaved Caspase-1, NO, IL-1β, IL-18), as well as LDH and the relative electrical conductivity were significantly augmented by BaP. The levels of these indices were increased by LY294002 pretreatment, and decreased by IGF-1. Similarly, LY294002 enhanced BaP-induced increase in the key protein expression of autophagy (Beclin-1 and LC3II), while IGF-1 weakened it. Finally, the phosphorylation of FOXO4 was clearly (p < 0.01) inhibited by BaP, and LY294002 suppressed this inhibitive effect of BaP, while IGF-1 strengthened it. In conclusion, BaP was able to induce pyroptotic and autophagic death via blocking the PI3K/Akt signaling pathway in HL-7702 liver cells.

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“…NO is a potent pro-inflammatory mediator, a low level is known to play an important role in cellular signaling under normal physiological condition. On the other hand, excessive production in the cell can lead to generation of peroxynitrite which subsequently damage the tissue [36,70]. Thus, high level of hepatic NO reported in this study (Fig.…”

Section: Discussionmentioning

confidence: 65%

“…This was performed as described by Ajayi et al [36]. Poly-L-lysine charged slides were rehydrated in xylene as well as decreasing concentration of ethanol (100e50%).…”

Section: Kidney Immunohistochemistrymentioning

confidence: 99%

Diallyl disulfide, a garlic-rich compound ameliorates trichloromethane-induced renal oxidative stress, NFkB activation and apoptosis in rats

Somade

Adedokun

Adeleke

et al. 2019

Clinical Nutrition Experimental

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Benzo(a)pyrene induces oxidative stress, pro-inflammatory cytokines, expression of nuclear factor-kappa B and deregulation of wnt/beta-catenin signaling in colons of BALB/c mice

Cited by 59 publications

References 65 publications

Potential of Inactivated Bifidobacterium Strain in Attenuating Benzo(A)Pyrene Exposure-Induced Damage in Colon Epithelial Cells In Vitro

Potential of Inactivated Bifidobacterium Strain in Attenuating Benzo(A)Pyrene Exposure-Induced Damage in Colon Epithelial Cells In Vitro

Benzo[a]pyrene induces pyroptotic and autophagic death through inhibiting PI3K/Akt signaling pathway in HL-7702 human normal liver cells

Diallyl disulfide, a garlic-rich compound ameliorates trichloromethane-induced renal oxidative stress, NFkB activation and apoptosis in rats

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