Oxygen uptake by roots of <i>Rumex</i> species at different temperatures: the relative importance of diffusive resistance and enzyme kinetics

Laan, Patrick; Tosserams, M.; Huys, P.; Bienfait, H. Frits

doi:10.1111/j.1365-3040.1991.tb01341.x

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…Soil temperatures in temperate regions are generally much lower than 24°C and temperature has been shown to affect O2 uptake considerably (Tab. 1), as was also found by Ibrahim et al (1983) and Laan et al (1991). Seeds in natural environments experience intermittent periods of hydration and drying.…”

Section: Survival Of Seeds In Soilsupporting

confidence: 77%

Changes in dormancy of Sisymbrium officinale seeds do not depend on changes in respiratory activity

Derkx¹,

Smidt²,

Plas³

et al. 1993

Physiol Plant

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Derkx, M. P M., Smidt, W. J., Van der Plas, L. H. W. and Karssen, C. M. 1993. Changes in dormancy of Sisymbrium officinale seeds do not depend on changes in respiratory activity. -Physiol. Plant. 89: 707-718.Effects of dark incubation at different temperatures were studied on dormancy and respiratory activity of seeds of Sisymbrium officinale (L.) Scop. Because germination of this species absolutely depends on the simultaneous action of light and nitrate, changes in dormancy could be studied in darkness without the interference of early germination events. Upon the start of incubation rates of O2 uptake and CO2 release rose. This was followed by a gradual decrease until stable levels of O2 uptake and CO2 release were achieved. Seeds kept for prolonged periods at 24°C, showed neither a change in germination capacity nor in rates of O2 uptake and CO2 release. Respiratory quotients were 0.55-0.7. The initial rise in O2 uptake correlated with the rate of water uptake and with breaking of primary dormancy. However, the subsequent decline in O2 uptake was not generally linked to induction of secondary dormancy. An increased O2 uptake was not required during breaking of secondary dormancy. It is concluded that changes in dormancy are not generally related to changes in respiratory activity. However, germination strongly depends on respiration. The increase in O2 uptake started well before radicle protrusion. A far red irradiation only reversed this increase when it was given before germination escaped from its red light antagonising action. The contribution of different respiratory pathways was followed during prolonged incubation at 24°C in darkness. KCN at 1.5 mM was needed to inhibit the eytochrome pathway (CP) and benzohydroxamic acid (BHAM) at 30 mM to inhibit the alternative pathway (AP). These concentrations did not exert any side effects. Electron flow was predominantly via the CP, maximally 10% was via the AP. Flow through the CP declined during the first 6 days and residual respiration remained constant. Therefore, the contribution of residual respiration became relatively more important with prolonged incubation. KCN at concentrations that almost completely inhibited flow through the CP, did not dramatically reduce germination. BHAM already inhibited germination at concentrations that do not inhibit oxygen uptake.

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Section: Survival Of Seeds In Soilsupporting

confidence: 77%

Changes in dormancy of Sisymbrium officinale seeds do not depend on changes in respiratory activity

Derkx¹,

Smidt²,

Plas³

et al. 1993

Physiol Plant

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“…Breeding and genetic modification for flood tolerance is common, and therefore it is important to understand the spatial and temporal dynamics of O 2 flux in the root environment (Bailey-Serres and Voesenek 2008). In addition to studying oxygen transport related to oxidative phosphorylation and photosynthesis, much research has focused on oxygen flux associated with alternative oxidase (Aox) (Laan et al 1991;Shi et al 2007;Gupta et al 2009;Polidoros et al 2009). Aox is regulated by mitochondrianucleus signaling, and is utilized as an electron overflow mechanism under both physiological and stressed conditions (Mclntosh 1994).…”

Section: Introductionmentioning

confidence: 99%

Self-referencing optrodes for measuring spatially resolved, real-time metabolic oxygen flux in plant systems

McLamore

Jaroch

Chatni

et al. 2010

Planta

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The ability to non-invasively measure metabolic oxygen flux is a very important tool for physiologists interested in a variety of questions ranging from basic metabolism, growth/development, and stress adaptation. Technologies for measuring oxygen concentration near the surface of cells/tissues include electrochemical and optical techniques. A wealth of knowledge was gained using these tools for quantifying real-time physiology. Fiber-optic microprobes (optrodes) have recently been developed for measuring oxygen in a variety of biomedical and environmental applications. We have adopted the use of these optical microsensors for plant physiology applications, and used the microsensors in an advanced sensing modality known as self-referencing. Self-referencing is a non-invasive microsensor technique used for measuring real-time flux of analytes. This paper demonstrates the use of optical microsensors for non-invasively measuring rhizosphere oxygen flux associated with respiration in plant roots, as well as boundary layer oxygen flux in phytoplankton mats. Highly sensitive/selective optrodes had little to no hysteresis/calibration drift during experimentation, and an extremely high signal-to-noise ratio. We have used this new tool to compare various aspects of rhizosphere oxygen flux for roots of Glycine max, Zea mays, and Phaseolus vulgaris, and also mapped developmentally relevant profiles and distinct temporal patterns. We also characterized real-time respiratory patterns during inhibition of cytochrome and alternative oxidase pathways via pharmacology. Boundary layer oxygen flux was also measured for a phytoplankton mat during dark:light cycling and exposure to pharamacological inhibitors. This highly sensitive technology enables non-invasive study of oxygen transport in plant systems under physiologically relevant conditions.

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“…Accordingly, significant size effects were found to modify tensile strength (Genet et al . 2005), oxygenation efficiency (Laan et al . 1991) and the hydraulic conductivity (Rieger & Litvin 1999) of root systems.…”

Section: Introductionmentioning

confidence: 99%

Trade‐off between root porosity and mechanical strength in species with different types of aerenchyma

Striker

Insausti

Grimoldi

et al. 2007

Plant Cell & Environment

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The objective of this work was to study the existence of a trade-off between aerenchyma formation and root mechanical strength. To this end, relationships among root anatomical traits and mechanical properties were analysed in plant species with contrasting root structural types: Paspalidium geminatum (graminaceous type), Cyperus eragrostis (cyperaceous type), Rumex crispus (Rumex type) and Plantago lanceolata (Apium type). Variations in anatomical traits and mechanical strength were assessed as a function of root diameter by exposing plants to 0, 7, 15 and 30 d of control and flooded conditions. For each species, the proportion of root cortex was positively associated with the increment of root diameter, contributing to the increase in root porosity under both control and flooded conditions. Moreover, cell lysis produced an additional increase in root porosity in most species under flooded conditions (except R. crispus). Both structural types that presented a uniseriate layer (epidermis) to cope with compression (Rumex and Apium types) were progressively weakened as root porosity increased. This effect was significant even when the increment of root porosity was solely because of increased root diameter (R. crispus), as when both processes (root diameter and cell lysis) added porosity to the roots (P. lanceolata). Conversely, structural types that presented a multiseriate ring of cells in the outer cortex (graminaceous and cyperaceous types) maintained mechanical strength over the whole range of porosity, in spite of lysogenic processes registered in the inner cortex. In conclusion, our study demonstrates a strong trade-off between aerenchyma formation and mechanical strength in root structural types that lacked a multiseriate ring of tissue for mechanical protection in the outer cortex. The results suggest that this ring of tissue plays a significant role in maintaining the mechanical strength of roots when flooding induces the generation of additional aerenchyma tissue in the root cortex.

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Oxygen uptake by roots of Rumex species at different temperatures: the relative importance of diffusive resistance and enzyme kinetics

Cited by 10 publications

References 22 publications

Changes in dormancy of Sisymbrium officinale seeds do not depend on changes in respiratory activity

Changes in dormancy of Sisymbrium officinale seeds do not depend on changes in respiratory activity

Self-referencing optrodes for measuring spatially resolved, real-time metabolic oxygen flux in plant systems

Trade‐off between root porosity and mechanical strength in species with different types of aerenchyma

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