Lotus corniculatus L. and Lotus glaber Mill. are warm-season legume species adapted to many kinds of environmental stress, including flooding conditions, whereas other popular forage legumes, like alfalfa or white clover, cannot thrive. This study evaluates the relationship between root aerenchyma, water relations and leaf gas exchange and the differential tolerance to soil flooding of L. corniculatus and L. glaber. Adult plants of these species, established independently in grasslands mesocosms, were subjected to 40 days of early spring flooding at a water depth of 6 cm. Both species presented constitutive aerenchyma tissue in the roots. Under flooding conditions, this parameter was 26.2% in L. glaber and 15.3% in L. corniculatus. In addition, flooded plants of L. glaber presented a leaf biomass 47.5% higher above water while L. corniculatus showed a leaf biomass 59.6% lower in the same layer, in comparison to control plants. Flooded plants of L. glaber maintained the stomatal conductance (g s ) and transpiration rate (E) for 25 days, although these parameters reduce slightly to 40À60% in comparison to controls after 40 days of flooding. In this species, a reduction in photosynthesis (A) in flooding conditions was detected only on the last day of measurement. In L. corniculatus, the same parameters (g s , E and A) were affected by flooding since day 18 of treatment, and values reached 25À40% in comparison to control plants after 40 days of flooding. Flooding did not affect above-ground biomass in L. glaber; while in L. corniculatus, above-ground biomass was 35% lower than in control plants. Our results confirmed that L. glaber is more able to cope with flooding stress than L. corniculatus, even in the presence of natural competitors. On the whole, this experiment provides information that can aid in the identification of anatomical and physiological parameters associated with flood-tolerance in this forage legume species, with economic potential for the agricultural areas subject to periodic flooding.
The objective of this work was to study the existence of a trade-off between aerenchyma formation and root mechanical strength. To this end, relationships among root anatomical traits and mechanical properties were analysed in plant species with contrasting root structural types: Paspalidium geminatum (graminaceous type), Cyperus eragrostis (cyperaceous type), Rumex crispus (Rumex type) and Plantago lanceolata (Apium type). Variations in anatomical traits and mechanical strength were assessed as a function of root diameter by exposing plants to 0, 7, 15 and 30 d of control and flooded conditions. For each species, the proportion of root cortex was positively associated with the increment of root diameter, contributing to the increase in root porosity under both control and flooded conditions. Moreover, cell lysis produced an additional increase in root porosity in most species under flooded conditions (except R. crispus). Both structural types that presented a uniseriate layer (epidermis) to cope with compression (Rumex and Apium types) were progressively weakened as root porosity increased. This effect was significant even when the increment of root porosity was solely because of increased root diameter (R. crispus), as when both processes (root diameter and cell lysis) added porosity to the roots (P. lanceolata). Conversely, structural types that presented a multiseriate ring of cells in the outer cortex (graminaceous and cyperaceous types) maintained mechanical strength over the whole range of porosity, in spite of lysogenic processes registered in the inner cortex. In conclusion, our study demonstrates a strong trade-off between aerenchyma formation and mechanical strength in root structural types that lacked a multiseriate ring of tissue for mechanical protection in the outer cortex. The results suggest that this ring of tissue plays a significant role in maintaining the mechanical strength of roots when flooding induces the generation of additional aerenchyma tissue in the root cortex.
We studied individual responses to flooding in the perennial grass Paspalum dilatatum , a widespread species in the Flooding Pampa of Argentina, using plants established in species-diverse grassland microcosms.• Flooding effects were evaluated on root and leaf sheath anatomy and shoot morphological traits. Leaf water status and CO 2 exchange rates were monitored in flooded and unflooded plants under changing, natural and controlled atmospheric conditions. • Root porosity and leaf sheath aerenchyma increased with flooding. Leaf extension rates and tiller height were also higher in flooded plants, which resulted in a large fraction of the shoot architecture emerging above the water surface. Flooding enhanced stomatal conductance, leaf water potential and net photosynthesis, especially under conditions leading to high air-vapour pressure deficits. Therefore, flooded plants experienced fewer water deficits during periods of high atmospheric evaporative demand. • P. dilatatum showed tight regulation of water and carbon relations under severe soil-oxygen deficiency, even in the presence of natural competitors. The suite of adaptive responses documented here might help to explain the observed increase in abundance of this species during extensive floods.
The forage legume L. tenuis has the flexibility either to escape from partial submergence by elongating its shoot more vigorously to avoid becoming totally submerged or to adopt a non-elongating quiescent strategy when completely immersed that is based on utilizing stored reserves. The possession of these alternative survival strategies helps to explain the success of L. tenuis in environments subjected to unpredictable flooding depths.
Summary 1.Knowledge of the regulatory effects of the crop canopy on weed seed germination is necessary to understand fully the behaviour of weed seed banks during a crop cycle. It is well known that canopy presence interferes with seed germination through modifications to the light and thermal environment, but the changing effect of a growing canopy has not been assessed, thus precluding the identification of shading-intensity thresholds for triggering the different types of canopy-detection mechanisms in different seed populations. 2.Field experiments were performed with artificially modified thermal and light environments, using two types of seed banks (seeds buried or located at the soil surface). Conditions below a wheat canopy were modified to match light and thermal conditions prevailing on bare soil (i.e. soil without vegetation). 3. Most weed emergence patterns during the early stages of crop establishment were not modified by the thermal regime produced by the incipient canopy compared with a bare soil control. However, the reduction of the red : far-red ratio from the bare soil value of 1·2-0·9 below the wheat canopy reduced germination of some weed species located at the soil surface, and the effect could by reversed by far-red filters. 4. The weed Galinsoga parviflora developed two generations during the crop cycle. The modified light and thermal environments beneath an establishing wheat canopy was not sufficient to inhibit the germination of Galinsoga parviflora , even if seeds were located at the soil surface. Only for seeds of the second generation, dispersed from seeds of the first plant generation, was there sufficient modification of the photothermal environment below the wheat canopy to interfere with dormancy termination. 5. Synthesis and applications . Understanding seed responses to modifications in the photothermal environment below a crop canopy (e.g. wheat crop) should allow us to improve weed management strategies by manipulating crop canopy attributes. This could be achieved by modification of sowing date, crop density, spatial arrangement and genotype. For example, increasing the crop plant density would diminish the number of weeds emerging during the first phase of crop establishment. This strategy would be appropriate where weed seeds are predominantly located at the soil surface, typically found in a no-till cropping system.
Strong fluctuations are exhibited by populations of the perennial herb Ambrosia tenuifolia in the grasslands of the Salado basin (Province of Buenos Aires, Argentina), an area frequently enduring prolonged floods. Flooding causes the death of most dicotyledon plants of the community, A. tenuifolia among them, opening numerous gaps of various sizes. After the recession of the flood the density of A. tenuifolia seedlings was higher in flooded than in non-flooded plots and it was larger in wider gaps. Canopy removal in non-flooded plots increased field seedling emergence of A. tenuifolia up to the levels found in flooded plots. Responses of the seeds in the soil to gap-associated environmental factors such as light quality and temperature regime were studied both in the field and under controlled were studied both in the field and under controlled conditions. Seedling emergence was significantly enhanced when the red:far-red ratio of natural light reaching the soil surface under the canopy of nonflooded plots was increased by means of copper sulfate filters. The influence of light quality and temperature on germination of the soil seed population was also tested using grassland soil monoliths or mesocosms, transported from the field to the laboratory, in which the canopy was clipped and the soil exposed to either red or far-red light and kept at constant or fluctuating temperatures. Significant seedling emergence was observed only when the soil samples were exposed to red light and incubated at alternating temperatures. No emergence was recorded in samples exposed to far-red light or incubated at a constant 25°C. Seeds stored dry in the laboratory were also stimulated to germinate by red light and alternating temperatures but only after dormancy was sufficiently decreased by low temperature stratification or by low temperature under immersion. The results are consistent with the hypothesis that primary dormancy of A. tenuifolia seeds is decreased by low temperatures in winter even if the seeds are submerged as happens when floods occur. The decrease in dormancy makes the seeds prone to be stimulated to germinate by the Pfr form of phytochrome in combination with alternating temperatures. These conditions are likely to be met in the gaps opened by the flood-caused death of dicotyledon plants.
Summary 1.We studied the differences in root strength of species with contrasting root structural types (the grass Paspalum dilatatum and the dicot Lotus glaber ), and their relationship with tolerance to simulated cattle trampling under flooding conditions. 2. Root strength was analysed through measurement of the pressure required to cause root collapse. The responses of aerenchyma and plant mass to flooding and trampling were studied. 3. Root aerenchyma increased from 28·0 to 40·2% in P. dilatatum and from 12·9 to 19·7% in L. glaber under flooding conditions. The increase in aerenchyma did not affect root strength in the relatively trampling-resistant roots of P. dilatatum : roots cracked at >380 kPa in all treatments. In contrast, roots of L. glaber were weaker, cracking at 260 kPa; flooded roots with air spaces irregularly dispersed in the cortex cracked at 115 kPa. 4. Trampling, flooding or their combination did not affect the biomass of P. dilatatum . Conversely, the isolated effects of either trampling or flooding both decreased biomass accumulation in L. glaber . The combination of both treatments killed all Lotus plants . 5. In conclusion, root strength was positively associated with soil trampling tolerance. The effect of aerenchyma tissue generation on root strength varies among root structural types. Aerenchyma tissue increases root weakness in the less stable structural type of the dicot species, but had no effect on the strength of the grass.
Natural flooding is one of the major factors affecting vegetation dynamics in many regions of the world. The Flooding Pampa Grasslands (Argentina) are frequently exposed to flooding events of diverse intensity and duration, some of which Leontodon taraxacoides, an exotic dicot. frequent in these grasslands, seems to survive. Its responses to four different water depths (0, 1, 7 and 13 cm) were studied. The results indicate that plants in conditions of total submergence (depth of 13 cm) did not survive. In less severe flood conditions, increases in the leaf insertion angle resulted in the maintenance of a large proportion of the total leaf area above the water. Differences in leaf length and a decrease in the width and the proportion of lobes per leaf were also found under partial submergence conditions (depth of 7 cm). Root and leaf aerenchyma, present in unflooded plants, showed a significant increase in flood conditions. In spite of the anatomical and morphological responses, total biomass and leaf area were severely affected by water depth. Control plants allocated more biomass to reproductive organs, while partly submerged plants allocated more to leaves and less to reproductive organs. Mature L. taraxacoides plants presented a wide range of plastic adjustment as a survival strategy in soil anaerobiosis, and appear to be able to survive short spring floods in a vegetative state ; in contrast, they might not tolerate total submergence conditions imposed by more intense and long-lasting floods.
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