Oxygen-Generating Nanofiber Cell Scaffolds with Antimicrobial Properties

Wang, Junping; Zhu, Yi‐Zhou; Bawa, Harinder K.; Ng, Geoffrey; Wu, Yong; Libera, Matthew; Mei, Henny C. van der; Busscher, Henk J.; Yu, Xiaojun

doi:10.1021/am100862h

Cited by 93 publications

(78 citation statements)

References 38 publications

(40 reference statements)

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“…Unfortunately, this strategy has a number of shortcomings including the possibility of systemic side-effects from the locally applied reactive chemicals or enzymes [13], local pH changes, and heating via exothermic reactions. Specific biomaterial strategies have been developed to blunt these potentially negative effects [9, 12, 14, 15], or avoid their need altogether including work using methacrylamide chitosan modified with perfluorocarbons (MACF) [16, 17]. …”

Section: Introductionmentioning

confidence: 99%

Fluorinated methacrylamide chitosan hydrogels enhance collagen synthesis in wound healing through increased oxygen availability

et al. 2016

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In this study, methacrylamide chitosan modified with perfluorocarbon chains (MACF) is used as the base material to construct hydrogel dressings for treating dermal wounds. MACF hydrogels saturated with oxygen (+ O2) are examined for their ability to deliver and sustain oxygen, degrade in a biological environment, and promote wound healing in an animal model. The emerging technique of metabolomics is used to understand how MACF + O2 hydrogel dressings improve wound healing. Results indicate that MACF treatment facilitates oxygen transport rate that is two orders of magnitude greater than base MAC hydrogels. MACF hydrogel dressings are next tested in an in vivo splinted rat excisional wound healing model. Histological analysis reveals that MACF + O2 dressings improve re-epithelialization (p < 0.0001) and synthesis of collagen over controls (p < 0.01). Analysis of endogenous metabolites in the wounds using global metabolomics demonstrates that MACF + O2 dressings promotes a regenerative metabolic process directed toward hydroxyproline and collagen synthesis, with confirmation of metabolite levels within this pathway. The results of this study confirm that increased oxygen delivery through the application of MACF + O2 hydrogels enhances wound healing and metabolomics analyses provides a powerful tool to assess wound healing physiology.

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Section: Introductionmentioning

confidence: 99%

Fluorinated methacrylamide chitosan hydrogels enhance collagen synthesis in wound healing through increased oxygen availability

et al. 2016

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“…1,2 Electrospun fibers mimic the extracellular matrix 3 and can be used as scaffolds for tissue engineering, [4][5][6] wound dressing, 7 drug release carriers, 8 or biosensors. 9 Antimicrobial activity can be introduced by doping with silver ions/nanoparticles, 10,11 peroxides, 12 photosensitizers, 13,14 or other chemical compounds. 15 Such materials can be suitable alternatives to antibiotics due to the increasing resistance of microorganisms, rendering conventional treatment less effective.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial nanofiber materials activated by light

Jesenská

Plíštil

Kubát

et al. 2011

J Biomedical Materials Res

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Electrospun polymeric nanofiber materials doped with 5,10,15,20-tetraphenylporphyrin (TPP) photosensitizer were prepared from four different polymers and were characterized with microscopic methods, steady-state, and time-resolved fluorescence and absorption spectroscopy. The polymers used included polyurethane Larithane™ (PUR), polystyrene (PS), polycaprolactone (PCL), and polyamide 6 (PA6). The antibacterial activity of all nanofiber materials against E. coli was activated by visible light and it was dependent on oxygen permeability/diffusion coefficients and the diameter of the polymeric nanofibers. This activity is based on oxidation ability of singlet oxygen O₂(¹Δ(g)) that is generated upon irradiation. All tested nanofiber materials exhibited prolonged antibacterial properties, even in the dark after long-duration irradiation. The post-irradiation effect was explained by the photogeneration of H₂O₂, which provided the material with long-lasting antibacterial properties.

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“…However, there are literature studies reporting the application of biomaterials loaded with peroxide containing compounds which, in most cases, utilize a catalyst to convert the peroxide to oxygen. These studies show that the H 2 O 2 containing materials when used in combination with the peroxide decomposition catalyst are biocompatible and non-toxic to cells and tissues (Camci-Unal et al, 2013;Harrison et al, 2007;Li et al, 2012;Oh et al, 2009;Wang et al, 2010). Fig.…”

Section: Shelf-life Of H 2 O 2 -H 2 O Loaded Pmma Microcapsulesmentioning

confidence: 75%

Hydrogen peroxide filled poly(methyl methacrylate) microcapsules: Potential oxygen delivery materials

Mallepally

Parrish

Hugh

et al. 2014

International Journal of Pharmaceutics

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This paper describes the synthesis of H₂O₂-H₂O filled poly(methyl methacrylate) (PMMA) microcapsules as potential candidates for controlled O₂ delivery. The microcapsules are prepared by a water-in-oil solvent emulsion and evaporation method. The results of this study describe the effect of process parameters on the characteristics of the microcapsules and on their in vitro performance. The size of the microcapsules, as determined from scanning electron microscopy, ranges from ∼5 to 30 μm and the size distribution is narrow. The microcapsules exhibit an internal morphology with entrapped H₂O₂-H₂O droplets randomly distributed in the PMMA continuous phase. In vitro release studies of 4.5 wt% H₂O₂-loaded microcapsules show that ∼70% of the H₂O₂ releases in 24h. This corresponds to a total O₂ production of ∼12 cc/gram of dry microcapsules. Shelf-life studies show that the microcapsules retain ∼84 wt% of the initially loaded H₂O₂ after nine months storage at 2-8 °C, which is an attractive feature for clinical applications.

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Oxygen-Generating Nanofiber Cell Scaffolds with Antimicrobial Properties

Cited by 93 publications

References 38 publications

Fluorinated methacrylamide chitosan hydrogels enhance collagen synthesis in wound healing through increased oxygen availability

Fluorinated methacrylamide chitosan hydrogels enhance collagen synthesis in wound healing through increased oxygen availability

Antibacterial nanofiber materials activated by light

Hydrogen peroxide filled poly(methyl methacrylate) microcapsules: Potential oxygen delivery materials

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