Oxidative stress, vitamin A and vitamin E behaviour in patients submitted to conservative surgery for complicated Crohn's disease

Lipid peroxidation markers in Crohn’s disease: the associations and diagnostic value

“…Consistently, it also tended to negatively correlate with catalase, an enzyme involved in hydrogen peroxide neutralization, the activity of which is diminished in CD (5,6) . In accordance with results of Sampietro et al (15) , reporting a direct MDA/TBARS association with severity of infl ammation, we found that MDA/TBARS to positively correlated with IL-6.…”

“…Although the process of lipid peroxidation seems relevant for the disease development, data on its markers in CD remain scanty and their diagnostic potential has not been evaluated before. Corroborating fi ndings of others (5,15,16) , we found the levels of MDA/TBARS to be increased in CD patients, more pronouncedly in active than inactive disease. Its levels directly corresponded with SOD1, the activity of which has previously been found up-regulated in erythrocytes (6) and leukocytes (5) of CD patients.…”

“…Such explanation is supported by results of others (15) , who also induced lipid peroxidation with cuprum sulfate but conducted the reaction for 8 h. They found no differences in TBARS following incubation between CD patients and healthy controls, although baseline TBARS have been signifi cantly elevated. As a result of prolonged incubation, the amount of peroxidation substrates in plasma, mostly polyunsaturated lipids (15) , defi ned PP evaluation in our study. Taking into account that the levels of polyunsaturated lipids have been shown to be depleted in active CD (17) , lower peroxidation potential can be expected.…”

“…Consequently, the process was still in its propagation phase. This initial step, "lag-time" representing plasma resistance to peroxidation conferred by antioxidants (15) , was a dominating factor in their evaluation of plasma susceptibility to peroxidation. Sampietro et al (15) showed signifi cantly shortened lag-time in CD patients resulting in earlier rapid increase of peroxidation products.…”

“…This initial step, "lag-time" representing plasma resistance to peroxidation conferred by antioxidants (15) , was a dominating factor in their evaluation of plasma susceptibility to peroxidation. Sampietro et al (15) showed signifi cantly shortened lag-time in CD patients resulting in earlier rapid increase of peroxidation products. This allowed Maor et al (16) to demonstrate higher plasma peroxidation potential in CD patients than controls.…”