Oxidative stress: potential of distinct peroxide determination systems

Abstract

When reactive oxygen species attack biological structures, peroxides, which are short-lived oxidative intermediates, are generated. We evaluated the potential of two different, commercially available peroxide activity assays (Pox-Act and d-ROMS) to see whether the results were associated with the clinical condition of subjects who were participating in a routine health care program. Furthermore, we determined the total antioxidant status (TAS) and the titer of autoantibodies against oxidized low-density lipoprotein (oLAb) to verify the hydroperoxide measurements. Subjects with medical conditions (hereafter referred to as patients) had significantly increased serum peroxide levels compared to healthy subjects. The d-ROMS kit indicated that 86% of subjects had an increased level of total peroxides. Although the assays had a significant correlation (p<0.001), 34% of the subjects had an increased total peroxide concentration in the Pox-Act assay that was clearly associated with clinical symptoms. Furthermore, the sensitivity of the Pox-Act assay was 35 times higher than that of the d-ROMS kit. In subjects with medical conditions, there was a trend toward a decreased TAS and a slightly increased oLAb titer in comparison to healthy subjects, but this was not statistically significant. The Pox-Act assay seems to be a valuable tool for the determination of total peroxides, while the results from the d-ROMS kit should be considered with caution.

Reactive Oxygen Metabolites: A Link between Oxidative Stress and Inflammation in Patients on Hemodialysis

“…Moreover, it is not affected by the significant changes of serum antioxidants and mainly those of uric acid concentration during dialysis, as applies to other TAC oxidative markers such as TAC and FRAP [5,22] . On the other hand, in a previous study the sensitivity of d-ROMs has been found lower in comparison with that of Pox-Act assay, which evaluates the concentration of total peroxides via a peroxidase reaction [23] . However, the diverse clinical profile of patients participating in the above study and the lack of evidence about a direct correlation of Pox-Act with other markers of oxidative stress, made the selection of d-ROMs preferable for our report, without excluding a prospective complementary use of the former test.…”

Persistent Oxidative Stress after Myocardial Infarction Treated by Percutaneous Coronary Intervention

“…This possibility seems credible because an almost identical pattern of progression of levels of sICAM-1 and total peroxides (r = 0.92) has been observed in our patients indicating a strong link between the two parameters. The determination of serum peroxides might therefore be simple and useful method for the estimation of the persistence of systemic oxidative stress during convalescence following severe acute disorders in cardiovascular systems, as also suggested by Lindschinger et al (2004).…”

Antioxidant Supplementation Attenuates Oxidative Stress in Patients Undergoing Coronary Artery Bypass Graft Surgery

“…The test system was based on a peroxide/peroxidase reaction using 3,5,3´,5´-tetramethylbenzidine (TMB) as substrate. Reference values were reported to be less than 200 µM (Lindschinger et al 2004). Peroxide levels were evaluated as arbitrary units with a CV of 3.73% (intra-assay variance) and 5.51% (inter-assay variance), and expressed as percentage of baseline.…”