Oxidative stress on mouse embryo development in vitro

Goto, Yasuo; Noda, Yoichi; Narimoto, Kazutaka; Umaoka, Yoh; Mori, Takahide

doi:10.1016/0891-5849(92)90165-d

Cited by 87 publications

(49 citation statements)

References 43 publications

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“…Therefore, IGF-I may promote embryonic development by an anti-apoptotic effect instead of increasing HIF-1α expression. Embryos cultured in vitro under low (5 %) oxygen tension have been reported to show higher developmental rates than those cultured under 20 % oxygen in mice [43], cattle [44], and human [23]. These reports indicated that a high oxygen tension during in vitro culture was found to be toxic to mammalian embryos, probably due to the formation of reactive oxygen species (ROS) [45,46].…”

Section: Discussionmentioning

confidence: 99%

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

Yoon

Juhn

et al. 2012

J Assist Reprod Genet

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Purpose Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O 2 in both the absence and presence of IGF-I. Results The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O 2 exhibited significantly higher total cell numbers (83.4±18.1) and lower apoptotic index (3.7±1.5) than those cultured under 20 % O 2 (67.4±15.6) (6.9±3.5) (P<0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-apoptotic effect, instead of increasing HIF-1α protein expression.

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Section: Discussionmentioning

confidence: 99%

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

Yoon

Juhn

et al. 2012

J Assist Reprod Genet

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“…Before implantation, the oxygen partial pressure in the lumen of the uterus settles between 20 and 37 mm Hg, regardless of the species of mammals considered [53,54]. However, from in vitro studies, it appears that, inside the cells of the embryo, the oxygen partial pressure is 10 to 5 times lower than outside the cells, so that the induced rate of ROS production is lower than that expected from the external oxygen partial pressure [50]. At implantation, oxygen partial pressure at the level of the cytotrophoblasts is thus of the order of 14 mm Hg, which corresponds to a level of 2% of oxygen in the atmosphere, i.e.…”

Section: Oxygen Partial Pressure As a Factor Of Ros Production Duringmentioning

confidence: 98%

“…It has been reported that oxygen partial pressure displays positive effects on embryo development only within a narrow range and that an oxidative stress can occur, with possibly potential lethal effects, below (slowing down of ATP synthesis) or above this range (direct peroxidation of membranes due to an excess of oxygen and radical chain initiation) [37]. In vitro, deviation of the oxygen partial pressures from the normal can induce oxidative stress and end embryo-toxicity if antioxidant enzymes (AOE) or antioxidant components are not provided [28,50,51]. During in utero embryo development, embryopathies occur, apparently due to oxygen induced ROS production, but in less well characterized circumstances [28,50,52].…”

Section: Oxygen Partial Pressure As a Factor Of Ros Production Duringmentioning

confidence: 99%

“…In vitro, deviation of the oxygen partial pressures from the normal can induce oxidative stress and end embryo-toxicity if antioxidant enzymes (AOE) or antioxidant components are not provided [28,50,51]. During in utero embryo development, embryopathies occur, apparently due to oxygen induced ROS production, but in less well characterized circumstances [28,50,52].…”

Section: Oxygen Partial Pressure As a Factor Of Ros Production Duringmentioning

confidence: 99%

“…An extracellular SOD (EC-SOD) [93], a ubiquitous thioredoxin [12,20] and peroxiredoxin, an enzyme controlling ROS concentration at the level of the nuclear membrane [49], are also important in the same respect. The seven enzymes, the activities of which are dependent on mineral trace elements, are essential for the positive outcome of the reproduction both in monogastrics and in ruminants [29,49,50,71,[94][95][96].…”

Section: Defense Against Ros and The Outcome Of Gestationmentioning

confidence: 99%

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Gestation linked radical oxygen species fluxes and vitamins and trace mineral deficiencies in the ruminant

2006

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-In mammals, radical oxygen species (ROS) are essential factors of cell replication, differentiation and growth (oxidative signal), notably during gestation, but are also potentially damaging agents. In Women, ROS play a role in remodeling of uterine tissues, implantation of the embryo, settlement of the villi and development of blood vessels characteristic of gestation. The body stores of vitamins and minerals of gestating females are used to keep ROS fluxes at a level corresponding to oxidative signals and to prevent an imbalance between their production and scavenging (oxidative stress), which would be detrimental to the mother and fetus. There is some evidence that, although based on different regulatory mechanisms, most of the effects of ROS reported in humans also occur in pregnant ruminant females, some of which have been actually reported. Many vitamins and trace elements have dual effects in the organism of mammals: (a) they are involved in the control of metabolic pathways or/and gene expression, (b) but most of the time they also display ROS trapping activity or their deficiencies induce high rates of ROS production. Deficiencies induce different disorders of gestation and can be induced by different kinds of stress. An example is given, corresponding to the decreased contents of cobalt of forages, when exposed to sustained heavy rains, so that the supply of vitamins B12 to the organism of the ruminant that grazes them is reduced and failure of gestation is induced. Outdoor exposure of ruminants to adverse climatic conditions by itself can increase the vitamin and trace element requirements. Adaptation of production systems taking into account these interactions between gestation and sources of stress or change of the quality of feeding stuffs as well as further developments of knowledge in that field is necessary to promote sustainable agricultural practices.gestation / ovine / bovine / vitamins / trace elements / antioxidant enzymes / radical oxygen species / radical phenomena

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