Assessment of DNA damage in individual hamster embryos by comet assay

Takahashi, Masashi; Saka, N.; Takahashi, Hitomi; Kanai, Yoshikatsu; Schultz, Richard M.; Okano, Akira

doi:10.1002/(sici)1098-2795(199909)54:1<1::aid-mrd1>3.0.co;2-0

Cited by 66 publications

(41 citation statements)

References 45 publications

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“…With the rapidly spreading use of time-lapse observations, especially on human embryos in fertility clinics, it is important to investigate, whether light exposure for image recording affects or even compromises the embryo's developmental competence. Different measures based on studies of animal model embryos have already been implemented to minimize light exposure of visible [15] or longer wavelength light [2,15,16], and in combination with sensitive camera systems it is possible to obtain high quality images for evaluation without disadvantages on embryonic development and quality [17]. Microscopy typically works with visible light (380-700 nm), where wavelengths <500-550 nm are regarded as harmful to the development and quality of mammalian embryos [2,15].…”

Section: Introductionmentioning

confidence: 99%

“…In mammalian cells, the function of fibroblast cells was disrupted when the total dose of blue light was increased to 10 kJ/m 2 [18]. However, most studies only use light intensity to quantify illumination effect on embryos [3,15,16]. Light at longer wavelength carries lower energy and red light is recommended as a safe illumination source for embryo observation systems [15] and is now routinely applied in timelapse incubation systems [11,12,[19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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Effect of red light on the development and quality of mammalian embryos

Pedersen

Liu

et al. 2014

J Assist Reprod Genet

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Purpose To assess irradiance and total energy dose from different microscopes during the in-vitro embryonic developmental cycle in mouse and pig and to evaluate its effect on embryonic development and quality in pig. Method Spectral scalar irradiance (380-1050 nm) was measured by a fiber-optic microsensor in the focal plane of a dissection microscope, an inverted microscope and a timelapse incubation system. Furthermore, the effect of three different red light levels was tested in the time-lapse system on mouse zygotes for 5 days, and on porcine zona-intact and zona-free parthenogenetically activated (PA) embryos for 6 days. Results The time-lapse system used red light centered at 625 nm and with a lower irradiance level as compared to the white light irradiance levels on the dissection and inverted microscopes, which included more energetic radiation <550 nm. Even after 1000 times higher total energy dose of red light exposure in the time-lapse system, no significant difference was found neither in blastocyst development of mouse zygotes nor in blastocyst rates and total cell number of blastocysts of porcine PA embryos. Conclusions Our results indicate that red light (625 nm, 0.34 W/ m 2 ) used in the time-lapse incubation system does not decrease the development and quality of blastocysts in both mouse zygotes and porcine PA embryos (both zona-intact and zona-free).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of red light on the development and quality of mammalian embryos

Pedersen

Liu

et al. 2014

J Assist Reprod Genet

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“…On the contrary, in vitro produced embryos as well as oocytes are prone to various environmental factors during manipulations in laboratories [1]. The adverse effects of pH fluctuations [2,3], high and low laboratory temperature [4], change in carbon dioxide and O 2 level [5,6] and light [7,8] have been investigated to some extent. Among the environmental factors, light is known to exert some harmful effects on developmental competence of mammalian oocytes and embryos [9].…”

Section: Introductionmentioning

confidence: 99%

“…Experiments on preimplantation rabbit embryos have shown that after exposure of embryos to 1,600 lx visible light for 8 h the development of day-1 embryos had decreased. In addition, development of hamster 1-cell embryos has also been impaired following exposure to visible light in a timedependant manner [8]. In contrast, mouse oocytes exposed to 4,000 lx visible light for 1 to 4 h had both normal fertilization and speed of cleavage [13].…”

Section: Introductionmentioning

confidence: 99%

Effect of embryonic fibroblast cell co-culture on development of mouse embryos following exposure to visible light

Nematollahi‐Mahani

Pahang

Moshkdanian

et al. 2009

J Assist Reprod Genet

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“…Similarly, heat stress-induced maternal hyperthermia enhances ROS production in the oviduct (Ozawa et al 2004, Matsuzuka et al 2005a, 2005b and lipid peroxidation in the liver (Matsuzuka et al 2005b). ROS are strongly reactive with cellular molecules and can cause serious dysfunction, such as enzyme inactivation (Halliwell & Gutteridge 1984), mitochondrial abnormalities (Kowaltowski & Vercesi 1999) and DNA fragmentation (Lopes et al 1998, Takahashi et al 1999.…”

Section: Introductionmentioning

confidence: 99%

DL- -Tocopherol acetate mitigates maternal hyperthermia-induced pre-implantation embryonic death accompanied by a reduction of physiological oxidative stress in mice

Sakamoto

Ozawa²,

Yokotani-Tomita³

et al. 2008

Reproduction

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Maternal hyperthermia induces pre-implantation embryo death, which is accompanied by enhanced physiological oxidative stress. We evaluated whether the administration of DL-a-tocopherol acetate (TA) to hyperthermic mothers mitigated pre-implantation embryo death. Mice were exposed to heat stress (35 8C, 60% relative humidity) for 12 h or not heated (25 8C) on the day of mating. Twelve hours before the beginning of temperature treatment, TA was injected intraperitoneally at a dose of 1 g/kg body weight. After the treatment, zygotes were recovered and the developmental abilities and intracellular glutathione (GSH) levels were evaluated. Another set of mice, with or without TA treatment, was exposed to heat stress for 12, 24 and 36 h, and the urinary levels of the oxidative stress marker 8-hydroxy-2 0 -deoxyguanosine (8-OHdG) were measured. Heat stress significantly decreased the blastocyst development rate and the GSH content in zygotes, as compared with the non-heat-stressed embryos, while TA administration significantly mitigated the deleterious effects of heat stress with regard to both parameters. Moreover, although the urinary levels of 8-OHdG gradually increased according to the duration of heat exposure, with or without TA administration, the levels were lower in the TA-administered group than in the placeboinjected mice. These results suggest that heat stress enhances physiological oxidative stress, and that TA administration alleviates the hyperthermia-induced death of pre-implantation embryos by reducing physiological oxidative stress.

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Assessment of DNA damage in individual hamster embryos by comet assay

Cited by 66 publications

References 45 publications

Effect of red light on the development and quality of mammalian embryos

Effect of red light on the development and quality of mammalian embryos

Effect of embryonic fibroblast cell co-culture on development of mouse embryos following exposure to visible light

DL- -Tocopherol acetate mitigates maternal hyperthermia-induced pre-implantation embryonic death accompanied by a reduction of physiological oxidative stress in mice

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