Oxidative Modifications Switch Modulatory Activities of Urinary Proteins From Inhibiting to Promoting Calcium Oxalate Crystallization, Growth, and Aggregation

Abstract

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Calcineurin B inhibits calcium oxalate crystallization, growth and aggregation via its high calcium-affinity property

“…Crystallization assay was employed to validate the effects of CNB on COM crystallization using lysozyme as the negative control. The results demonstrated that total urinary proteins (TUPs) from healthy subjects, that are known to strongly inhibit COM stone formation [17] , markedly reduced COM crystal size, number and abundance comparing with blank and negative controls ( Fig. 2 ).…”

“…TUPs were prepared from healthy subjects as described previously [17] . The study protocol met the standard guidelines (Declaration of Helsinki, Belmont Report, and ICH Good Clinical Practice) and was approved by Siriraj Institutional Review Board (approval no.…”

Application of metabolomics in urolithiasis: the discovery and usage of succinate

“…The results from crystallization assay indicated that succinic, salicyluric acid and gentisic acid could decrease the size and number of the crystals, which was consistent with previous studies of inhibitor for COM crystallization. 43 , 44 During crystallization, succinic, salicyluric acid and gentisic acid can shift COM towards COD crystallization, which may confer a therapeutic effect as COD has lower affinity to epithelial cell membrane molecules and may thereby reduce intratubular retention. 11 , 45 , 46 …”

Caffeine causes cell cycle arrest at G0/G1 and increases of ubiquitinated proteins, ATP and mitochondrial membrane potential in renal cells

“…Protein concentrations were measured using Bio-Rad protein assay (Bio-Rad; Milano, Italy) based on Bradford’s method. An equal amount (30 µg) of total proteins from each sample was subjected to in-solution tryptic digestion as described previously [18] , [19] . The digested peptides were then analyzed by nanoLC-ESI-LTQ-Orbitrap MS/MS as previously reported [20] , [21] .…”