Oxidative DNA Damage Estimated by Plasma 8‐hydroxydeoxyguanosine (8‐OHdG): Influence of 4, 4'‐methylenebis (2‐chloroaniline) Exposure and Smoking

Sunlight Exposure–Mediated DNA Damage in Young Adults

“…We carried out binary logistic regression analysis with creatinine-adjusted urinary 8-OHdG level (>8 mg/g creatinine) as the dependent variable and sunlight exposure time per day (hours per day), ratio of sunlight-exposed skin area to the whole body, sunlight intensity (mW/cm 2 ), L*-value (lightness) in the skin, and regular use of full-spectrum (i.e., UVA and UVB) sunscreen as independent variables (Table 1). Models were adjusted for age, sex, smoking, drinking, and BMI because correlations between urinary 8-OHdG levels and these factors have been reported (18,(21)(22)(23). We used the SPSS (version 18) software package (SPSS Japan …”

Rapid and simple one-step membrane extraction for the determination of 8-hydroxy-2′-deoxyguanosine in human plasma by a combination of on-line solid phase extraction and LC–MS/MS

“…8-OHdG has been associated with several diseases, such as aging [3], cancer neurodegenerative diseases [1], diabetes [4], Alzheimer's disease [5], cardiomyopathy [6] and cardiovascular or infectious diseases [7]. Recently, quantification of 8-OHdG has been suggested as a reliable indicator of oxidative stress in biological fluids including plasma [8], serum [9], urine [10][11][12], saliva [13], and in tissue [14][15][16]. It can be regarded as a sensitive and stable biomarker of DNA oxidative damage to estimate the toxicity of internal and external factors.…”

“…When the concentration is quite low, a large sample volume and endogenous components (salts, amine, fatty acid and triglycerides, etc.) are needed [8,24]. Therefore, we developed a new one-step membrane extraction for a sample preparation method and combined it with on-line SPE-LC-MS/MS, which was rapid, automated, a small sample volume with a decreased matrix effect that provided accurate, precise measurements of 8-OHdG in human plasma samples.…”

Effect ofCYP3A4genetic polymorphisms on the genotoxicity of 4,4′-methylene-bis(2-chloroaniline)-exposed workers

“…These workers are protected with long gloves and a respirator at work. We divided the study population into an exposure group (urine total MBOCA ≥20 μg/g creatinine) and a control group (urine total MBOCA <20 μg/g creatinine) by the total MBOCA concentration in urine 17. The exposure group consisted of 44 workers, and the control group consisted of 47 workers.…”