Rapid and simple one-step membrane extraction for the determination of 8-hydroxy-2′-deoxyguanosine in human plasma by a combination of on-line solid phase extraction and LC–MS/MS

Wang, Chien-Jen; Yang, Ning-Hsiang; Chang, Chia-Chi; Liou, Saou‐Hsing; Lee, Hui‐Ling

doi:10.1016/j.jchromb.2011.09.038

Cited by 34 publications

(18 citation statements)

References 30 publications

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“…Some authors point that the levels of 8-oxodG in serum samples profiled by ELISA and by HPLC differ substantially [93, 94]. In our opinion, most likely explanation of these discrepancies is the fact that, in serum, 8-oxodG circulates both as free nucleoside and as part of oxidized cfDNA fragments.…”

Section: Extracellular Dna Is Enriched With the Oxidized Genomic Dnamentioning

confidence: 91%

“…In our opinion, most likely explanation of these discrepancies is the fact that, in serum, 8-oxodG circulates both as free nucleoside and as part of oxidized cfDNA fragments. ELISA quantifies total concentrations of 8-oxodG that, in healthy donors, is around 0.3–5.9 ng/mL of plasma/serum [95–99] while HPLC-based techniques detect only free 8-oxodG that is present in plasma/serum samples at concentrations of 0.013–0.022 ng/mL [93, 94, 100–103]. One may calculate the minimal background content of 8-oxodG embedded in cfDNA fragments by subtracting bound 8-oxodG concentrations from total 8-oxodG concentrations (0.3–0.022 ≈ 0.28 ng/mL).…”

Section: Extracellular Dna Is Enriched With the Oxidized Genomic Dnamentioning

confidence: 99%

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Oxidized Extracellular DNA as a Stress Signal in Human Cells

Ermakov

Konkova

Kostyuk

et al. 2013

Oxidative Medicine and Cellular Longevity

106

113

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The term “cell-free DNA” (cfDNA) was recently coined for DNA fragments from plasma/serum, while DNA present in in vitro cell culture media is known as extracellular DNA (ecDNA). Under oxidative stress conditions, the levels of oxidative modification of cellular DNA and the rate of cell death increase. Dying cells release their damaged DNA, thus, contributing oxidized DNA fragments to the pool of cfDNA/ecDNA. Oxidized cell-free DNA could serve as a stress signal that promotes irradiation-induced bystander effect. Evidence points to TLR9 as a possible candidate for oxidized DNA sensor. An exposure to oxidized ecDNA stimulates a synthesis of reactive oxygen species (ROS) that evokes an adaptive response that includes transposition of the homologous loci within the nucleus, polymerization and the formation of the stress fibers of the actin, as well as activation of the ribosomal gene expression, and nuclear translocation of NF-E2 related factor-2 (NRF2) that, in turn, mediates induction of phase II detoxifying and antioxidant enzymes. In conclusion, the oxidized DNA is a stress signal released in response to oxidative stress in the cultured cells and, possibly, in the human body; in particular, it might contribute to systemic abscopal effects of localized irradiation treatments.

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Section: Extracellular Dna Is Enriched With the Oxidized Genomic Dnamentioning

confidence: 91%

Section: Extracellular Dna Is Enriched With the Oxidized Genomic Dnamentioning

confidence: 99%

Oxidized Extracellular DNA as a Stress Signal in Human Cells

Ermakov

Konkova

Kostyuk

et al. 2013

Oxidative Medicine and Cellular Longevity

106

113

View full text Add to dashboard Cite

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“…Urine samples were immediately frozen for later solid phase extraction and LC-MS analysis for 8-OHdG. [21] Blood samples were centrifuged; and plasma aliquots were stored at −30 °C before analysis for sCD62P, CRP, and VWF using ELISA methods (R&D Systems for CRP and sCD62P; RayBiotech for VWF). Breath sample collection and analyses, as well as spirometry and PWA measurements, were conducted using standard procedures.…”

Section: Methodsmentioning

confidence: 99%

Combined use of an electrostatic precipitator and a high-efficiency particulate air filter in building ventilation systems: Effects on cardiorespiratory health indicators in healthy adults

Day

Xiang

et al. 2018

Indoor Air

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High-efficiency particulate air (HEPA) filtration in combination with an electrostatic precipitator (ESP) can be a cost-effective approach to reducing indoor particulate exposure, but ESPs produce ozone. The health effect of combined ESP-HEPA filtration has not been examined. We conducted an intervention study in 89 volunteers. At baseline, the air-handling units of offices and residences for all subjects were comprised of coarse, ESP, and HEPA filtration. During the 5-week long intervention, the subjects were split into 2 groups, 1 with just the ESP removed and the other with both the ESP and HEPA removed. Each subject was measured for cardiopulmonary risk indicators once at baseline, twice during the intervention, and once 2 weeks after baseline conditions were restored. Measured indoor and outdoor PM and ozone concentrations, coupled with time-activity data, were used to calculate exposures. Removal of HEPA filters increased 24-hour mean PM exposure by 38 (95% CI: 31, 45) μg/m . Removal of ESPs decreased 24-hour mean ozone exposure by 2.2 (2.0, 2.5) ppb. No biomarkers were significantly associated with HEPA filter removal. In contrast, ESP removal was associated with a -16.1% (-21.5%, -10.4%) change in plasma-soluble P-selectin and a -3.0% (-5.1%, -0.8%) change in systolic blood pressure, suggesting reduced cardiovascular risks.

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“…Therefore, the 8-OHdG levels are considered to be a valid marker of oxidative DNA damage. In addition, new techniques have been introduced into this field as gas chromatography with mass spectrometry [17,25], electrospray ionization mass spectrometry (MS) [26], liquid chromatography with mass spectrometry [27][28][29][30] and capillary electrophoresis [31][32][33][34][35][36]. From the direct ones, high performance liquid chromatography (HPLC) with electrochemical detection (ED) [23,24] belongs to the most commonly used method for determination of 8-OHdG.…”

Section: Determination Of 8-ohdgmentioning

confidence: 99%

Electrochemical Study of DNA Damaged by Oxidation Stress

Zítka¹,

Křížková²,

Skaličková³

et al. 2013

Combinatorial Chemistry & High Throughput Screening

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Many compounds can interact with DNA leading to changes of DNA structure as point mutation and bases excision, which could trigger some metabolic failures, which leads to the changes in DNA structure resulting in cancer. Oxidation of nucleic acid bases belongs to the one of the mostly occurred type of DNA damaging leading to the above mentioned phenomena. The investigation of processes of DNA oxidation damage is topical and electrochemical methods include a versatile and sensitive tool for these purposes. 8-hydroxydeoxyguanosine (8-OHdG) is the most widely accepted marker of DNA damage. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generate several other products within the double helix besides mentioned oxidation products of nucleic acid bases. The basic electrochemical behaviour of nucleic acids bases on various types of carbon electrodes is reviewed. Further, we address our attention on description of oxidation mechanisms and on detection of the most important products of nucleic bases oxidation. The miniaturization of detector coupled with some microfluidic devices is suggested and discussed. The main aim of this review is to report the advantages and features of the electrochemical detection of guanine oxidation product as 8-OHdG and other similarly produced molecules as markers for DNA damage.

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Rapid and simple one-step membrane extraction for the determination of 8-hydroxy-2′-deoxyguanosine in human plasma by a combination of on-line solid phase extraction and LC–MS/MS

Cited by 34 publications

References 30 publications

Oxidized Extracellular DNA as a Stress Signal in Human Cells

Oxidized Extracellular DNA as a Stress Signal in Human Cells

Combined use of an electrostatic precipitator and a high-efficiency particulate air filter in building ventilation systems: Effects on cardiorespiratory health indicators in healthy adults

Electrochemical Study of DNA Damaged by Oxidation Stress

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