Oxidative Damage and Enhanced Erythrophagocytosis in Canine Erythrocytes Infected with Babesia gibsoni.

Murase, Toshiyuki; Ueda, Takeo; Yamato, Osamu; Tajima, Motoshi; Maede, Yoshimitsu

doi:10.1292/jvms.58.259

Cited by 55 publications

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“…In infectious diseases, the activation of macrophages and production of reactive oxygen species (ROS) for phagocytizing the agent are shown as important causes of increased oxidative stress (18). In addition, it has been found that, in dogs with babesiosis, reactive species produced by the agent as well as the direct breakdown of erythrocytes also contribute to oxidative stress and anemia (4,23). Oxidative stress in dogs with leishmaniasis has also been associated with chronic renal failure and uremia caused by the disease (1).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of oxidative status in dogs with anemia

Gültekin

Voyvoda

2017

Medycyna Weterynaryjna

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SummaryThe aim of the present study was to evaluate the relationship between the oxidative status and the severity and type of anemia in dogs. A total of 70 dogs of various breeds, ages and of both sexes were enrolled in the study. Fifty dogs with anemia were classified according to the severity of anemia as mildly (n = 18), moderately (n = 18) or severely (n = 14) anemic on the basis of the hematocrit (HCT) value. Anemia in the same dogs was also classified according to the type as regenerative (n = 26) or non-regenerative (n = 24) on the basis of the absolute reticulocyte count. Twenty dogs were used as healthy control. Total oxidant status (TOS), total antioxidant capacity (TAC) and malondialdehyde (MDA) values in plasma as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities in erythrocyte hemolysate were measured to evaluate the oxidative status. The plasma TOS value was increased in all anemic dogs, irrespective of the severity of anemia, whereas a significant increase in the TAC value and a significant decrease in erythrocyte GSH-Px activity were observed in mild and moderate anemia. Plasma TOS and TAC values were higher in dogs with both types of anemia when compared to the healthy group. A significant increase in plasma MDA concentration and a significant decrease in GSH-Px activity were observed in the non-regenerative anemia group. There were moderate negative correlations between HCT and TOS values in the mild anemia group and between HCT and erythrocyte GSH-Px activity in the regenerative anemia group. In conclusion, oxidative stress develops in dogs with anemia, and it is largely independent of the severity and type of anemia. These results suggest that further studies with different etiologies may also be useful for evaluating the efficacy of antioxidants administered at different doses and in different combinations to treat anemia in dogs.

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Section: Resultsmentioning

confidence: 99%

Evaluation of oxidative status in dogs with anemia

Gültekin

Voyvoda

2017

Medycyna Weterynaryjna

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“…An increased level of MDA, an end product of lipid peroxidation, has been reported in B. gibsoni infection (Murase et al 1996;Chaudhuri et al 2008), in Canine Distemper infection (Karadeniz et al 2008), in dogs with coccidiosis (Kizil and Yuce 2009) and in canine visceral leishmaniasis (Heidarpour et al 2012). The present findings are in agreement with these reports.…”

Section: Discussionmentioning

confidence: 98%

Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus

Kayar¹,

Dokuzeylül²,

Kandemır³

et al. 2015

Vet. Med. - Czech

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Feline coronavirus (FCoV) is a highly contagious virus that is ubiquitous in multicat environments and may induce oxidative stress. This virus commonly causes an asymptomatic infection, which can persist in certain individuals. Sporadically and unpredictably, FCoV infection leads to feline infectious peritonitis (FIP), a highly fatal systemic immune-mediated disease. There are no data in the veterinary literature relating to oxidative stress in FCoV. Antioxidant capacity (TAC) can be attributed to single components in the defence systems against free radicals. The measurement of the total oxidant status (TOS) accurately reflects the oxidative status of blood plasma or serum. Nitric oxide (NO) acts as a free radical and contributes to host defences against oxidation. Malondialdehyde (MDA) is a reliable and commonly used marker of overall lipid peroxidation levels and the presence of oxidative stress. This study aimed to determine levels of oxidative stress markers, serum TAC, total oxidant capacity (TOC), NO and serum MDA in 24 cats seropositive for FCoVs and 15 cats seronegative for FCoVs. Significantly higher serum TOC, NO and MDA levels were found in seropositive animals (P < 0.001, P < 0.05 and P < 0.001, respectively) than in seronegative animals. In contrast, serum TAC levels were found to be significantly lower in seropositive cats compared with seronegative cats (P < 0.001). The results of the present study suggest that FCoV seropositivity is associated with oxidative stress and decreased antioxidant status.

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“…This early change was hypothesized to be caused by hemodilution, splenomegaly and sequestration in the spleen (Maegraith et al, 1957;Schetters et al, 1997aSchetters et al, , 1998Schetters et al, , 2009). This mechanism may include autoimmune haemolysis (Reyers et al, 1998), reduced red cell deformability (Dondrop et al, 1999) and increased oxidative damage (Murase et al, 1995;Morita et al, 1996;Otsuka et al, 2001;Rezaei and Dalir-Naghadeh, 2006). A possible role of the highly reactive oxygen free radicals in the pathogenesis of parasitic infections has been an active area of research over the past ten years (Biswas et al, 1997;Erel et al, 1997;Oliveira and Cechini, 2002).…”

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confidence: 99%

Malondialdehyde levels in serum of dogs infected with Babesia canis

Crnogaj¹,

Petlevski²,

Mrljak³

et al. 2010

Vet. Med.

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Babesiosis is a common tick born disease of dogs in tropical and subtropical regions of the world caused by different species of Babesia. The aim of the present study was to confirm the presence of oxidative stress by examining serum malondialdehyde (MDA), an end product of lipid peroxidation, in 35 dogs naturally infected with Babesia canis (B. canis). MDA was examined in 14 healthy dogs as well. Blood smears were prepared from peripheral blood and they showed the presence of B. canis in infected dogs. B. canis was confirmed using the PCR (Polymerase chain reaction) method. On the basis of clinical and laboratory data the 35 infected dogs were clinically classified into two groups, complicated (seven dogs) and uncomplicated (28 dogs). The noted complications were renal dysfunction (5/7), hepatic dysfunction (3/7), muscular involvement (2/7) and ARDS (1/7). Levels of blood urea nitrogen concentration (BUN), creatinin, total bilirubin, alanin aminotranspherase (ALT), alkaline phosphatase (AP), and gammaglutamil aminotranspherase (GGT) were significantly increased in dogs with complicated versus uncomplicated babesiosis. Furthermore the uncomplicated group of dogs was, depending on the severity of anaemia, classified as suffering from severe, moderate or mild disease. Levels of serum MDA were significantly higher in sick dogs (36.90 μmol/l ± 13.95) than healthy animals (8.13 μmol/l ± 1.78). There was no significant statistical difference in serum MDA levels between dogs with complicated (38.48 μmol/l ± 12.11) and uncomplicated babesiosis (36.50 μmol/l ± 14.55). Comparison of the groups based on the severity of anaemia showed that there was no significant statistical difference in serum MDA levels between them. The study demonstrated the involvement of oxidative damage in dogs naturally infected with B. canis.

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Oxidative Damage and Enhanced Erythrophagocytosis in Canine Erythrocytes Infected with Babesia gibsoni.

Cited by 55 publications

References 0 publications

Evaluation of oxidative status in dogs with anemia

Evaluation of oxidative status in dogs with anemia

Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus

Malondialdehyde levels in serum of dogs infected with Babesia canis

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