Oxidase Activity of Tissues Systemically Infected by Tobacco Mosaic Virus

Merrett, M. J.

doi:10.1111/j.1399-3054.1962.tb08051.x

Cited by 7 publications

(2 citation statements)

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“…Polyphenoloxidase activity increases when local lesion hosts are infected with viruses and this increased activity has been considered a primary cause of lesion formation (Solymosy, Farkas & Kiraly, 1959) and of the increased oxygen uptake of the tissue (Millerd & Scott, 1962); evidence against both these viewpoints was given by Merrett (1960). Also, healthy tissue from between lesions of inoculated leaves showed the same rate of respiration as healthy tissue (Table I), although van Kammen & Rrouwer (1964) have shown an increase in polyphenol oxidase activity in these tissues.…”

Section: Discussionmentioning

confidence: 99%

The respiration of leaves showing necrotic local lesions following infection by tobacco mosaic virus

Sunderland

Merrett

1965

Annals of Applied Biology

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At 2 5 ' C. the respiration rate of leaves of Nicotiana tabacum L. var. Xanthi infected with tobacco mosaic virus increased before the appearance of local lesions. The increase was directly proportional to lesion number at low, but not at higher, lesion densities. With temperatures at which the infected leaf showed necrotic lesions the difference in respiration rates between healthy and infected leaves was greater than at 35' C., at which temperature infected leaves do not develop necrotic lesions. The concentration of a,q-dinitrophenol (DNP) that gave maximum stimulation of the respiration of the healthy leaf was IO-~M, but even this respiration rate was less than that of the infected leaf showing local lesions. DNP had no effect on the respiration of the infected leaf showing a dense lesion cover. At 35' C., DNP increased the respiration rate of infected leaves and the maximum respiration rates for healthy and infected leaves in the presence of DNP were similar. MATERIALS A N D METHODS Plants of Nicotiana tabacum L. var. Xanthi were grown in a glasshouse at a mean temperature of 19' C. Fully expanded leaves were used in all experiments. Leaves of the same age on different plants were selected for controls and for inoculation with virus. The other leaves on the plant were not removed. The virus inoculum was the expressed sap of leaves of N . tabacum L. var. White Burley infected with TMV. The

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Section: Discussionmentioning

confidence: 99%

The respiration of leaves showing necrotic local lesions following infection by tobacco mosaic virus

Sunderland

Merrett

1965

Annals of Applied Biology

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“…Such decreased rate of respiration may correspond to either a completion of invasion or to the rising of accumulated virus to an appreciable fraction of the dry weight of leaves as indicated by OWEN (1956). It has been suggested by MERRETT (1962) that such decrease may be due to utilisation of nucleotides in virus synthesis and reduction of phosphate acceptors available for oxidative phosphorylation.…”

Section: Discussionmentioning

confidence: 98%