Ovine panel reactive antibody assay of HLA responsivity to allograft bioengineered vascular scaffolds

Kreuger, Paula; Lukoff, Howard; Robinson, Elliot; Jones, Alyce Linthurst; Crouch, Katrina; Wolfinbarger, Lloyd; Hopkins, Richard A.

doi:10.1016/j.jtcvs.2004.06.017

Cited by 24 publications

(17 citation statements)

References 19 publications

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“…One of the major advantages of decellularized matrices is their reduced immunogenicity, which has been reported in heart valve, 10 blood vessel, 26,27 cardiac defect, 28 and trachea. 29 The decellularized tissues have demonstrated reduced levels of HLA panelreactive antibodies when compared with those in fresh or cryopreserved tissue.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells

Ozeki

Narita

Kagami

et al. 2006

J Biomedical Materials Res

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Recently, decellularized tissue has been reported to have the potential to regenerate a variety of tissues. However, the optimal protocol for a decellularized esophagus has not been studied. Here, we investigated the effect of different decellularization protocols on the histology and biocompatibility of decellularized esophagi in view of future applications to tissue engineering. The esophageal mucosal epithelium (EP) from 4-week-old Wistar rats was enzymatically dissociated and cultured with growth-arrested feeder cells. Two methods for decellularization using deoxycholic acid (DEOX) or Triton X-100 (TRITON) were compared on esophagi from adult Wistar rats. Those treated with DEOX showed superior mechanical properties, maintenance of extracellular matrix, and lower DNA content than those treated with TRITON. To evaluate the biocompatibility of the scaffold, cultured (passage 3) esophageal epithelial cells were seeded inside the decellularized esophagus and cultured for 7 days. The cells seeded onto the decellularized esophagus were examined histologically and immunocytochemically. Esophageal epithelial cells were stratified into three to four cellular layers in vitro inside the decellularized esophagus, to show polarity. The results from immunocytochemistry indicated that the seeded epithelial cells expressed characteristic marker proteins for native esophageal EP. Decellularized esophagus showed suitable compatibility as a scaffold material for esophageal tissue engineering.

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Section: Discussionmentioning

confidence: 99%

“…Several recent investigations have focused on methods to avoid immunogenic reactions as well as possible infection from decellularized matrices. 26,28,33 The newly developed protocols for decellularization may help to avoid such problems and serve to create an adequate decellularized esophagus.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells

Ozeki

Narita

Kagami

et al. 2006

J Biomedical Materials Res

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“…Human or "processed" xenograft scaffolds are almost certainly more proinflammatory when cells are disrupted and cellular debris, cytokines, and other inflammatory moieties are not thoroughly removed from the matrix. 17 Such concerns are supported by the studies of Rieder et al 16 They demonstrated that the lowest level of stimulation was with thoroughly "decellularized" human tissues. In fact, the decellularized porcine leaflets were far more attractive (stimulated macrophage response) than the extracts of human native pulmonary cusps that had not been decellularized.…”

Section: See P 2792mentioning

confidence: 96%

“…16 This has been previously suggested by in vitro and in vivo animal studies and human assays. [17][18][19] The unfortunate clinical experiment with the implantation of decellularized xenograft heart valves (Synergraft) before the completion of sufficient preregulatory studies only emphasized the risk of proceeding down pathways absent full understanding of the potential for ECM provocation of the immune as well as the innate nonspecific inflammatory responses. 20 In fact, a "decellularized" xenograft scaffold may actually be more inflammatory than present versions of cryopreserved homografts (ie, with retained donor cells).…”

Section: See P 2792mentioning

confidence: 99%

Tissue Engineering of Heart Valves

Hopkins

2005

Circulation

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“…Decellularization removes the host cellular antigens, reducing the inflammatory response and minimizing immune-mediated tissue rejection [66], leaving a tubular arrangement of extracellular matrix (ECM) [67]. It is the premise that ECM proteins can integrate into the surrounding tissue [68] that makes this approach attractive.…”

Section: Decellularized Scaffoldsmentioning

confidence: 99%

Tissue Engineering a Small Diameter Vessel Substitute: Engineering Constructs with Select Biomaterials and Cells

McBane

Sharifpoor²,

Labow³

et al. 2012

CVP

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Cardiovascular disease (CVD) is a leading cause of death and hospitalization worldwide. The need for small caliber vessels ( < 6mm) to treat CVD patients has grown; however the availability of autologous vessels in cardiac and peripheral bypass candidates is limited. The search for an alternative vessel source is widespread with both natural and synthetic tissue engineered materials being investigated as scaffolds. Despite decades of exhaustive studies with decellularized extracellular matrices (ECM) and synthetic graft materials, the field remains in search of a commercially viable biomaterial construct substitute. While the previous materials have been assessed by evaluating their compatibility with fibroblasts, smooth muscle cells and endothelial cells, current materials are being conceived based on their interactions with stem cells, progenitor cells and monocytes, as the latter may hold the key to repair and regeneration. The graft's ability to recruit and maintain these cells has become a major research focus. The successful tissue engineering of a small caliber vessel graft requires the use of optimal material chemistry and biological function to promote cell recruitment into the graft while maintaining each functional phenotype during vessel tissue maturation. The discussion of these significant research challenges constitutes the focus of this review.

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Ovine panel reactive antibody assay of HLA responsivity to allograft bioengineered vascular scaffolds

Abstract: These studies with an ovine modified panel reactive antibody assay confirmed minimal immune allosensitization to transplanted decellularized tissue patches. Qualifying criteria for putative tissue-engineered scaffolds should include minimal recipient panel reactive antibody response.

Cited by 24 publications

References 19 publications

Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells

Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells

Tissue Engineering of Heart Valves

Tissue Engineering a Small Diameter Vessel Substitute: Engineering Constructs with Select Biomaterials and Cells

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