Tissue Engineering a Small Diameter Vessel Substitute: Engineering Constructs with Select Biomaterials and Cells

McBane, Joanne E.; Sharifpoor, Soroor; Labow, Rosalind S.; Ruel, Marc; Suuronen, Erik J.; Santerre, J. Paul

doi:10.2174/157016112799959378

Cited by 27 publications

(26 citation statements)

References 162 publications

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“…As drawbacks, these scaffolds impair VSMC infiltration, making the re-cellularization process hard and time-dispersive [19]. Moreover, decellularized biological tissues do not overcome the availability issue, while synthetic scaffolds offer favorable advantages, as higher reproducibility and both controlled microstructure [24] and degradation rate [25,26]. On one hand, porous scaffolds able to promote VSMCs colonization from the external side towards the lumen are required [27,28].…”

Section: Introductionmentioning

confidence: 99%

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Tresoldi

Pacheco

Formenti

et al. 2019

Materials Science and Engineering: C

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Aiming to perfuse porous tubular scaffolds for vascular tissue engineering (VTE) with controlled flow rate, prevention of leakage through the scaffold lumen is required. A gel coating made of 8% w/v alginate and 6% w/v gelatin functionalized with fibronectin was produced using a custom-made bioreactor-based method. Different volumetric proportions of alginate and gelatin were tested (50/50, 70/30, and 90/10). Gel swelling and stability, and rheological, and uniaxial tensile tests reveal superior resistance to the aggressive biochemical microenvironment, and their ability to withstand physiological deformations (~10%) and wall shear stresses (5-20 dyne/cm 2). These are prerequisites to maintain the physiologic phenotypes of vascular smooth muscle cells and endothelial cells (ECs), mimicking blood vessels microenvironment. Gels can induce ECs proliferation and colonization, especially in the presence of fibronectin and higher percentages of gelatin. The custom-designed bioreactor enables the development of reproducible and homogeneous tubular gel coating. The permeability tests show the effectiveness of tubular scaffolds coated with 70/30 alginate/gelatin gel to occlude wadding pores, and therefore prevent leakages. The synthesized double-layered tubular scaffolds coated with alginate/gelatin gel and fibronectin represent both promising substrate for ECs and effective leakproof scaffolds, when subjected to pulsatile perfusion, for VTE applications.

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Section: Introductionmentioning

confidence: 99%

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Tresoldi

Pacheco

Formenti

et al. 2019

Materials Science and Engineering: C

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“…When seeded on electrospun scaffolds these same cells displayed poor surface coverage that would severely diminish an engineered tissue’s barrier function. This would be particularly detrimental for engineered blood vessels where platelet aggregation on exposed biomaterial surfaces results in thrombosis and loss of patency [34–36]. Because both scaffolds had similar cell adhesion, this lack of coverage suggests that cells have migratedinto the scaffold which is acceptable for 2D tissue formation.…”

Section: Discussionmentioning

confidence: 99%

“…Pore casting looks to overcome these problems by replacing PDMS with non-deformable, solvent-friendly silicon to produce thin scaffolds with minimum topography and user-defined pore size. These scaffolds may be particularly attractive for the development of small-diameter vascular tissue engineering which has been stymied by thrombogenicity, patency, and bursting [34–41]. …”

Section: Introductionmentioning

confidence: 99%

A novel porous scaffold fabrication technique for epithelial and endothelial tissue engineering

McHugh

Tao

Saint‐Geniez

2013

J Mater Sci: Mater Med

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Porous scaffolds have the ability to minimize transport barriers for both two- (2D) and three-dimensional tissue engineering. However, current porous scaffolds may be non-ideal for 2D tissues such as epithelium due to inherent fabrication-based characteristics. While 2D tissues require porosity to support molecular transport, pores must be small enough to prevent cell migration into the scaffold in order to avoid non-epithelial tissue architecture and compromised function. Though electrospun meshes are the most popular porous scaffolds used today, their heterogeneous pore size and intense topography may be poorly-suited for epithelium. Porous scaffolds produced using other methods have similar unavoidable limitations, frequently involving insufficient pore resolution and control, which make them incompatible with 2D tissues. In addition, many of these techniques require an entirely new round of process development in order to change material or pore size. Herein we describe “pore casting,” a fabrication method that produces flat scaffolds with deterministic pore shape, size, and location that can be easily altered to accommodate new materials or pore dimensions. As proof-of-concept, pore-cast poly(ε-caprolactone) (PCL) scaffolds were fabricated and compared to electrospun PCL in vitro using canine kidney epithelium, human colon epithelium, and human umbilical vein endothelium. All cell types demonstrated improved morphology and function on pore-cast scaffolds, likely due to reduced topography and universally small pore size. These results suggest that pore casting is an attractive option for creating 2D tissue engineering scaffolds, especially when the application may benefit from well-controlled pore size or architecture.

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“…Although autologous veins or arteries are currently the preferred option, the availability of suitable native vessels is frequently in limited supply or restricted dimensions due to pre-existing vascular diseases or disease progression, 1 thus there is an increasing demand for synthetic vascular grafts accompanied by climbing incidences of coronary and peripheral artery diseases. 2 Unfortunately, owing to acute thrombosis and intimal hyperplasia, graft patency of synthetic conduits in small-diameter situations is disappointing so far, 3 therefore, how to improve the long-term patency rate of small-diameter artificial blood vessels remains a major challenge and opportunity for vascular tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency

Wang¹,

Mo²,

Jiang³

et al. 2013

IJN

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Abstract:The poor patency rate following small-diameter vascular grafting remains a major hurdle for the widespread clinical application of artificial blood vessels to date. Our previous studies found that electrospun poly(L-lactide-co-epsilon-caprolactone) (P[LLA-CL]) nanofibers facilitated the attachment and growth of endothelial cells (EC), and heparin incorporated into P(LLA-CL) nanofibers was able to release in a controlled manner. Hence, we hypothesized that heparin-bonded P(LLA-CL) vascular scaffolds with autologous EC pre-endothelialization could significantly promote the graft patency rate. To construct a small-diameter vascular scaffold, the inner layer was fabricated by heparin-bonded P(LLA-CL) nanofibers through coaxial electrospinning, while the outer layer was woven by pure P(LLA-CL) nanofibers. Except dynamic compliance (5.4 ± 1.7 versus 12.8 ± 2.4 × 10 −4 /mmHg, P , 0.05), maximal tensile strength, burst pressure, and suture retention of the composite, scaffolds were comparable to those of canine femoral arteries. In vitro studies indicated that the scaffolds can continuously release heparin for at least 12 weeks and obtain desirable endothelialization through dynamic incubation, which was confirmed by EC viability and proliferation assay and scanning electronic microscopy. Furthermore, in vivo studies demonstrated that pre-endothelialization by autologous ECs provided a better effect on graft patency rate in comparison with heparin loading, and the united application of pre-endothelialization and heparin loading markedly promoted the 24 weeks patency rate of P(LLA-CL) scaffolds (88.9% versus 12.5% in the control group, P , 0.05) in the canine femoral artery replacement model. These results suggest that heparin-bonded P(LLA-CL) scaffolds have similar biomechanical properties to those of native arteries and possess a multiporous and biocompatible surface to achieve satisfactory endothelialization in vitro. Heparin-bonded P(LLA-CL) scaffolds with autologous EC pre-endothelialization have the potential to be substitutes for natural small-diameter vessels in planned vascular bypass surgery.

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Tissue Engineering a Small Diameter Vessel Substitute: Engineering Constructs with Select Biomaterials and Cells

Cited by 27 publications

References 162 publications

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

A novel porous scaffold fabrication technique for epithelial and endothelial tissue engineering

Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency

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