Overlap of epitopes recognized by anti-carbonic anhydrase I IgG in patients with malignancy-related aplastic anemia-like syndrome and in patients with aplastic anemia

Jankovičová, Barbora; Škultéty, Ľudovít; Dubrovčáková, Mária; Stangel, Martin; Bı́lková, Zuzana; Lakota, Ján

doi:10.1016/j.imlet.2013.07.006

Cited by 9 publications

(20 citation statements)

References 17 publications

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“…The tryptic peptides were analyzed by automated nanoflow reverse-phase chromatography using the nanoAcquity UPLC system coupled to a Q-TOF Premier (Waters, USA) as described earlier (Skultety et al, 2011 ; Jankovicova et al, 2013 ; Flores-Ramirez et al, 2014 ). Peptides were injected onto a reverse-phase column (Waters nanoAcquity UPLC column BEH 130 C18, 75 μm × 150 mm, 1.7 μm particle size).…”

Section: Methodsmentioning

confidence: 99%

Partially resistant Cucurbita pepo showed late onset of the Zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar

et al. 2015

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Zucchini yellow mosaic virus (ZYMV) is an emerging viral pathogen in cucurbit-growing areas wordwide. Infection causes significant yield losses in several species of the family Cucurbitaceae. To identify proteins potentially involved with resistance toward infection by the severe ZYMV-H isolate, two Cucurbita pepo cultivars (Zelena susceptible and Jaguar partially resistant) were analyzed using a two-dimensional gel electrophoresis-based proteomic approach. Initial symptoms on leaves (clearing veins) developed 6–7 days post-inoculation (dpi) in the susceptible C. pepo cv. Zelena. In contrast, similar symptoms appeared on the leaves of partially resistant C. pepo cv. Jaguar only after 15 dpi. This finding was confirmed by immune-blot analysis which showed higher levels of viral proteins at 6 dpi in the susceptible cultivar. Leaf proteome analyses revealed 28 and 31 spots differentially abundant between cultivars at 6 and 15 dpi, respectively. The variance early in infection can be attributed to a rapid activation of proteins involved with redox homeostasis in the partially resistant cultivar. Changes in the proteome of the susceptible cultivar are related to the cytoskeleton and photosynthesis.

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Section: Methodsmentioning

confidence: 99%

Partially resistant Cucurbita pepo showed late onset of the Zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar

et al. 2015

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“…Repeated autoantibody findings point to the involvement of the humoral immunity in the induction and/or maintenance of autoimmunity in IAA. Previously, IAA-associated autoantibodies have been detected against kinectin [ 21 , 22 ], diazepam-binding inhibitor-related protein 1 (DRS-1) [ 23 ], carbonic anhydrase 1 (CA-1) [ 24 , 25 ], heterogeneous nuclear ribonucleoprotein (hnRNP) K [ 26 ], chloride intracellular channel 1 (CLIC1), heat shock binding protein (HSBP11), ribosomal protein S27 (RPS27) [ 27 ], moesin [ 28 ] and postmeiotic segregation increased 1 (PMS-1) [ 22 ]. Most of these antibodies are not specific for IAA, but are also detected in other autoimmune, BMF or inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

Anti-COX-2 autoantibody is a novel biomarker of immune aplastic anemia

et al. 2022

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In immune aplastic anemia (IAA), severe pancytopenia results from the immune-mediated destruction of hematopoietic stem cells. Several autoantibodies have been reported, but no clinically applicable autoantibody tests are available for IAA. We screened autoantibodies using a microarray containing >9000 proteins and validated the findings in a large international cohort of IAA patients (n = 405) and controls (n = 815). We identified a novel autoantibody that binds to the C-terminal end of cyclooxygenase 2 (COX-2, aCOX-2 Ab). In total, 37% of all adult IAA patients tested positive for aCOX-2 Ab, while only 1.7% of the controls were aCOX-2 Ab positive. Sporadic non-IAA aCOX-2 Ab positive cases were observed among patients with related bone marrow failure diseases, multiple sclerosis, and type I diabetes, whereas no aCOX-2 Ab seropositivity was detected in the healthy controls, in patients with non-autoinflammatory diseases or rheumatoid arthritis. In IAA, anti-COX-2 Ab positivity correlated with age and the HLA-DRB1*15:01 genotype. 83% of the >40 years old IAA patients with HLA-DRB1*15:01 were anti-COX-2 Ab positive, indicating an excellent sensitivity in this group. aCOX-2 Ab positive IAA patients also presented lower platelet counts. Our results suggest that aCOX-2 Ab defines a distinct subgroup of IAA and may serve as a valuable disease biomarker.

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“…5 After the epitope mapping, the anti-CA I antibodies in this group of patients (bona fide AA) recognized the same linear candidates CA I epitopes-DGLAV, NVGHS, SLKPI and SSEQL. 6 Antibodies against CA I have been presented in some autoimmune diseases, for example, systemic lupus erythematosus, Sjögren's syndrome, autoimmune/idiopathic chronic pancreatitis, connective tissue diseases and other rheumatic diseases (reviewed previously in Ref. [6]).…”

Section: Autoimmune Phenomena and Spontaneous Tumour Regression The Role Of Carbonic Anhydrase I Dear Editormentioning

confidence: 99%

Autoimmune phenomena and spontaneous tumour regression. The role of carbonic anhydrase I

Minichová

Škultéty

Lakota

2021

J Cellular Molecular Medi

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LETTER TO THE EDITOR relapsed/progressed after HDC and ASCT and achieved spontaneous tumour regression (ie without any further treatment). The epitope 'alignment' in these cases is not only surprising, but it is also challenging. Will we go further, prepare monoclonal therapeutic antibodies, and possibly will be able to treat a wide variety of malignant tumours? ACK N OWLED G EM ENTSThis work was supported by the grant APVV 18-0340 and Foundation for Cell Transplantation.

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Overlap of epitopes recognized by anti-carbonic anhydrase I IgG in patients with malignancy-related aplastic anemia-like syndrome and in patients with aplastic anemia

Cited by 9 publications

References 17 publications

Partially resistant Cucurbita pepo showed late onset of the Zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar

Partially resistant Cucurbita pepo showed late onset of the Zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar

Anti-COX-2 autoantibody is a novel biomarker of immune aplastic anemia

Autoimmune phenomena and spontaneous tumour regression. The role of carbonic anhydrase I

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