Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas

Shigeishi, Hideo; Fujimoto, Shinichi; Hiraoka, Misato; Ono, Shigehiro; Taki, Masayuki; Ohta, Kouji; Higashikawa, Koichiro; Kamata, Nobuyuki

doi:10.3892/ijo_00000286

Cited by 37 publications

(32 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TPX2 was initially reported to be a Xenopus microtubule-associated protein (27). TPX2 is a cell cycle protein and serves a key role in the stability of the mitotic spindle (18).…”

Section: Discussionmentioning

confidence: 99%

TPX2 in human clear cell renal carcinoma: Expression, function and prognostic significance

et al. 2016

View full text Add to dashboard Cite

Abstract. Targeting protein for Xenopus kinesin-like protein 2 (TPX2) is a microtubule-associated protein. TPX2 is considered to be an important gene in tumorigenesis; however, the particular function of TPX2 in the development of human renal cell carcinoma (RCC) is unknown. In the present study, the expression, function and prognostic significance of TPX2 in human RCC was analyzed. A total of 286 tissue samples from patients with RCC who had undergone nephrectomies were utilized. Subsequently, the expression of TPX2 protein was investigated using immunohistochemistry and western blotting, and TPX2 mRNA expression was examined using reverse transcription-quantitative polymerase chain reaction. To establish the effect of TPX2 on the proliferation and invasion of the RCC cells, TPX2 expression was increased by stable transfection with a TPX2 vector and TPX2 expression was decreased using small interfering RNA. Proliferation of the RCC cells was analyzed using a WST-1 assay and an animal xenograft model with BALB/c nude mice, whilst invasion of the RCC cells was examined using a Matrigel-coated invasion chamber. It was demonstrated that TPX2 expression was significantly higher in the RCC tissues compared with normal kidney tissues (P<0.05). Furthermore, TPX2 expression was associated with tumor size, histological grade and tumor stage (P<0.05), and was observed to markedly increase the proliferation and invasion of the RCC cells. It may be concluded that the expression of TPX2 is significantly upregulated in RCC tissue, subsequently increasing the proliferative and invasive ability of RCC cells. Therefore, the protein may serve as a therapeutic target and independent prognostic factor in the treatment of human RCC.

show abstract

“…TPX2 was initially reported to be a Xenopus microtubule-associated protein (27). TPX2 is a cell cycle protein and serves a key role in the stability of the mitotic spindle (18).…”

Section: Discussionmentioning

confidence: 99%

TPX2 in human clear cell renal carcinoma: Expression, function and prognostic significance

et al. 2016

View full text Add to dashboard Cite

show abstract

“…TPX2 was found to be upregulated in squamous cell carcinoma of the lung with the expression correlating to tumor grade, stage and nodal status (4). The high-expression of TPX2 has been demonstrated in other cancers such as ovarian cancer (10) breast cancer (5) pancreatic cancer (11), meningioma (12), astrocytoma (13), mantle cell lymphoma (14), hepatocellular carcinoma (15), oral squamous cellcarcinomas (16), and giant-cell tumor of the bone (17). The high-expression of TPX2 has predictive value for the biological behavior of the disease, and can be used as a prognostic indicator.…”

Section: Discussionmentioning

confidence: 99%

The TPX2 gene is a promising diagnostic and therapeutic target for cervical cancer

et al. 2012

View full text Add to dashboard Cite

Abstract. The target protein for Xklp2 (TPX2), a microtubuleassociated protein, can be used to evaluate more precisely the proliferative behavior of tumor cells. The abnormal expression of TPX2 in various types of malignant tumors has been reported, but less is known for cervical cancer. We studied the relationship between TPX2 expression and the biological behavior of cervical cancer. Immunohistochemistry and RT-PCR were used to detect the expression of TPX2 in cervical cancer tissues. The inhibitory effect of TPX2-siRNA on the growth of HeLa human cervical carcinoma cells was studied in vitro. TPX2 expression was found to be significantly higher in cervical carcinoma compared to normal cervical tissues and CIN. The expression of TPX2 in cervical cancer was correlated with histological grading, FIGO staging and lymph node metastasis. TPX2 RNAi in HeLa cervical cancer cells caused S-phase cell cycle arrest, induced apoptosis and inhibited cell proliferation and invasion. In conclusion, TPX2 shows potential to be used as a new marker for cervical cancer diagnosis and therapy.

show abstract

“…Recently, we identified that RHAMM was overexpressed in oral squamous cell carcinomas. 22 The overexpression of RHAMM has also been observed in other types of cancers. [23][24][25] In oral squamous cell carcinomas, RHAMM expression was correlated with the expression of TPX2 (targeting protein for X. laevis kinesinlike protein 2), encoding a protein which is required for the formation of microtubules at kinetochores in mammalian cells.…”

mentioning

confidence: 99%

RHAMM/ERK interaction induces proliferative activities of cementifying fibroma cells through a mechanism based on the CD44–EGFR

Hatano

Shigeishi

Kudo

et al. 2011

Laboratory Investigation

View full text Add to dashboard Cite

We have previously established immortalized cells (HCF) from cementifying fibroma of the jaw bone. Here, we found that the receptor for hyaluronan (HA)-mediated motility (RHAMM) and epiregulin, a ligand for the epidermal growth factor receptor (EGFR), were highly expressed in HCF cells in comparison with osteoblasts by conducting a microarray analysis. The cell growth of HCF cells was significantly decreased by the knockdown of RHAMM using small interfering RNA (siRNA). RHAMM was associated with extracellular signal-regulated kinase (ERK) and essential for ERK phosphorylation. HCF cells had characteristic growth mechanisms in which epiregulin functions in an extracellular autocrine loop. Interestingly, exogenous HA induced the phosphorylation of EGFR, which was mainly dependent on CD44. The results raise the novel idea that the EGFR may activate Raf-MEK-ERK signaling in response to the binding of HA to CD44. Moreover, RHAMM was able to associate with TPX2 in the nucleus and was required for HA-induced activation of the Aurora A kinase. The results suggest that RHAMM has a predominant role in the cell cycle in HCF. Here, we report the new machinery by which RHAMM/ERK interaction induces the proliferative activity of cementifying fibroma cells via a specific signaling pathway through the CD44-EGFR axis. Benign fibro-osseous diseases in the oral and maxillofacial regions can be divided into three categories: fibrous dysplasia, osseous dysplasia, and cementifying (ossifying) fibroma.

show abstract

Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas

Cited by 37 publications

References 19 publications

TPX2 in human clear cell renal carcinoma: Expression, function and prognostic significance

TPX2 in human clear cell renal carcinoma: Expression, function and prognostic significance

The TPX2 gene is a promising diagnostic and therapeutic target for cervical cancer

RHAMM/ERK interaction induces proliferative activities of cementifying fibroma cells through a mechanism based on the CD44–EGFR

Contact Info

Product

Resources

About