The TPX2 gene is a promising diagnostic and therapeutic target for cervical cancer

Abstract

Abstract. The target protein for Xklp2 (TPX2), a microtubuleassociated protein, can be used to evaluate more precisely the proliferative behavior of tumor cells. The abnormal expression of TPX2 in various types of malignant tumors has been reported, but less is known for cervical cancer. We studied the relationship between TPX2 expression and the biological behavior of cervical cancer. Immunohistochemistry and RT-PCR were used to detect the expression of TPX2 in cervical cancer tissues. The inhibitory effect of TPX2-siRNA on the growth of HeLa human cervical carcinoma cells was studied in vitro. TPX2 expression was found to be significantly higher in cervical carcinoma compared to normal cervical tissues and CIN. The expression of TPX2 in cervical cancer was correlated with histological grading, FIGO staging and lymph node metastasis. TPX2 RNAi in HeLa cervical cancer cells caused S-phase cell cycle arrest, induced apoptosis and inhibited cell proliferation and invasion. In conclusion, TPX2 shows potential to be used as a new marker for cervical cancer diagnosis and therapy.

Interplay Between Spindle Architecture and Function

“…Given the critical role of the spindle, it is not surprising that many associated factors have been linked to diseases in which chromosome segregation is disrupted (Noatynska et al, 2012). For example, a significant percentage of MAPs that affect spindle assembly or function have altered expression levels at the mRNA or protein level in a variety of cancers (Bieche et al, 2011;Chang et al, 2012;Wang et al, 2010). Chromosome segregation errors in female meiosis also pose a significant problem and lead to the high incidence of human miscarriage and developmental diseases such as Down syndrome (trisomy 21; Hassold and Hunt, 2001).…”

Mitotic Stress and Chromosomal Instability in Cancer: The Case for TPX2

“…Specifically, TPX2 knockdown induces cell cycle arrest, apoptosis, and the inhibition of cell proliferation and invasion in cervical, prostate, hepatocellular, and pancreatic cancer cell lines. [83][84][85][86] siRNA-mediated knockdown of TPX2 inhibits the proliferation and growth of hepatocellular and pancreatic cancer cell xenografts transplanted into immunodeficient mice. 85,86 Furthermore, TPX2 downregulation has been shown to sensitize pancreatic cancer cells to paclitaxel treatment.…”

Targeting Protein for Xenopus Kinesin-like Protein 2 (TPX2) Regulates γ-Histone 2AX (γ-H2AX) Levels upon Ionizing Radiation

“…Moreover, TPX2 levels are altered in cancers associated with aberrant cellular responses to DNA damage and genomic instability (12)(13)(14)(15)(16)(17)(18)(19). These observations raise the question as to whether TPX2 is involved in the DNA damage response.…”

“…), and amplification of the TPX2 gene has been suggested to promote the progression of colorectal malignancies (12)(13)(14)(15)(16)(17)(18)(19). Conversely, TPX2 haploinsufficiency, leading to decreased levels of TPX2, significantly increases the propensity for the development of tumors in mice (20).…”