Overexpression of SmMYC2 Increases the Production of Phenolic Acids in Salvia miltiorrhiza

Yang, Na; Zhou, Wenping; Su, Jie; Wang, Xiaofan; Li, Lin; Wang, Liru; Cao, Xiaoyan; Wang, Zhezhi

doi:10.3389/fpls.2017.01804

Cited by 80 publications

(56 citation statements)

References 50 publications

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“…We have reported that overexpression of SmMYC2 strongly increases the production of RA and Sal B, and those transcript levels of SmTAT1 and SmPAL1 are dramatically improved in SmMYC2 -OE lines ( Yang et al, 2017 ). However, the molecular mechanism had not yet been characterized.…”

Section: Resultsmentioning

confidence: 99%

“…To evaluate whether the OE box had been integrated into the transgenic plant genome, we amplified the CaMV35S promoter from isolated genomic DNA, using previously published protocols ( Yang et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…The biosynthetic pathway of Sal B consists of a phenylalanine-derived pathway and tyrosine-derived pathway ( Di et al, 2013 ; Ma et al, 2013 ; Wang et al, 2015 ). In view of the economic value and clinical demand for this active ingredient, biological approaches have been taken to augment its synthesis, including the engineering of genes in the biosynthetic pathway and ectopic expression of transcription factors (TFs) ( Zhang et al, 2010 , 2014 ; Wang et al, 2013 ; Zhou et al, 2016 ; Yang et al, 2017 ). For example, AtPAP1 from Arabidopsis thaliana is a transcriptional activator of phenolic acid biosynthesis in S. miltiorrhiza ( Zhang et al, 2010 , 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, the functions of those genes have not been characterized. We previously reported that overexpression of SmMYC2 increases the production of phenolic acids in S. miltiorrhiza ( Yang et al, 2017 ). Further investigation showed that constitutive expression of that gene significantly up-regulates transcript levels of SmbHLH37 ( Su et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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SmbHLH37 Functions Antagonistically With SmMYC2 in Regulating Jasmonate-Mediated Biosynthesis of Phenolic Acids in Salvia miltiorrhiza

Niu

et al. 2018

Front. Plant Sci.

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Jasmonates (JAs) are integral to various defense responses and induce biosynthesis of many secondary metabolites. MYC2, a basic helix-loop-helix (bHLH) transcription factor (TF), acts as a transcriptional activator of JA signaling. MYC2 is repressed by the JASMONATE ZIM-domain (JAZ) proteins in the absence of JA, but de-repressed by the protein complex SCFCOI1 on perception of JA. We previously reported that overexpression of SmMYC2 promotes the production of salvianolic acid B (Sal B) in Salvia miltiorrhiza. However, the responsible molecular mechanism is unclear. Here, we showed that SmMYC2 binds to and activates the promoters of its target genes SmTAT1, SmPAL1, and SmCYP98A14 to activate Sal B accumulations. SmbHLH37, a novel bHLH gene significantly up-regulated by constitutive expression of SmMYC2, was isolated from S. miltiorrhiza for detailed functional characterization. SmbHLH37 forms a homodimer and interacts with SmJAZ3/8. Overexpression of SmbHLH37 substantially decreased yields of Sal B. SmbHLH37 binds to the promoters of its target genes SmTAT1 and SmPAL1 and blocks their expression to suppress the pathway for Sal B biosynthesis. These results indicate that SmbHLH37 negatively regulates JA signaling and functions antagonistically with SmMYC2 in regulating Sal B biosynthesis in S. miltiorrhiza.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SmbHLH37 Functions Antagonistically With SmMYC2 in Regulating Jasmonate-Mediated Biosynthesis of Phenolic Acids in Salvia miltiorrhiza

Niu

et al. 2018

Front. Plant Sci.

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“…The transcriptome database revealed 21 genes homologous to SmPAL, SmTAT, SmC4H, and SmRAS which were upregulated. The most essential of them (SmPAL) in the phenylpropanoid pathway was up-regulated 367.1-fold [22].…”

Section: Manipulation Of Secondary Metabolism Through Metabolic Enginmentioning

confidence: 99%

Green (cell) factories for advanced production of plant secondary metabolites

Marchev

Yordanova

Georgiev

2020

Critical Reviews in Biotechnology

113

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For centuries plants have been intensively utilized as reliable sources of food, flavoring, agrochemical and pharmaceutical ingredients. However, plant natural habitats are being rapidly lost due to climate change and agriculture. Plant biotechnology offers a sustainable method for the bioproduction of plant secondary metabolites using plant in vitro systems. The unique structural features of plant-derived secondary metabolites, such as their safety profile, multi-target spectrum and "metabolite likeness," have led to the establishment of many plant-derived drugs, comprising approximately a quarter of all drugs approved by the Food and Drug Administration and/or European Medicinal Agency. However, there are still many challenges to overcome to enhance the production of these metabolites from plant in vitro systems and establish a sustainable large-scale biotechnological process. These challenges are due to the peculiarities of plant cell metabolism, the complexity of plant secondary metabolite pathways, and the correct selection of bioreactor systems and bioprocess optimization. In this review, we present an integrated overview of the possible avenues for enhancing the biosynthesis of high-value marketable molecules produced by plant in vitro systems. These include metabolic engineering and CRISPR/Cas9 technology for the regulation of plant metabolism through overexpression/repression of single or multiple structural genes or transcriptional factors. The use of NMR-based metabolomics for monitoring metabolite concentrations and additionally as a tool to study the dynamics of plant cell metabolism and nutritional management is discussed here. Different types of bioreactor systems, their modification and optimal process parameters for the labor industrial-scale production of plant secondary metabolites are specified.

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Salvia miltiorrhiza: An Economically and Academically Important Medicinal Plant

2019

Compendium of Plant Genomes

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Overexpression of SmMYC2 Increases the Production of Phenolic Acids in Salvia miltiorrhiza

Cited by 80 publications

References 50 publications

SmbHLH37 Functions Antagonistically With SmMYC2 in Regulating Jasmonate-Mediated Biosynthesis of Phenolic Acids in Salvia miltiorrhiza

SmbHLH37 Functions Antagonistically With SmMYC2 in Regulating Jasmonate-Mediated Biosynthesis of Phenolic Acids in Salvia miltiorrhiza

Green (cell) factories for advanced production of plant secondary metabolites

Salvia miltiorrhiza: An Economically and Academically Important Medicinal Plant

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