Overexpression of Small Heat Shock Protein LimHSP16.45 in Arabidopsis Enhances Tolerance to Abiotic Stresses

Mu, Cuicui; Zhang, Shijia; Yu, Guanzhong; Chen, Ni; Li, Xiaofeng; Liu, Heng

doi:10.1371/journal.pone.0082264

Cited by 90 publications

(64 citation statements)

References 43 publications

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“…However, only single alleles were tested, and no complementation experiments were performed. Further evidence for the stress-protective role of CI and CII sHSPs in plants has primarily involved constitutive expression or overexpression of a single sHSP in different plant species, including Arabidopsis, rice (Oryza sativa), maize (Zea mays), and carrot (Daucus carota), as well as others (Malik et al, 1999;Ahn and Zimmerman, 2006;Jiang et al, 2009;Sun et al, 2012;Zhou et al, 2012;Mu et al, 2013;Wang et al, 2015). A very restricted set of stress-resistant phenotypes has been reported for sHSP-overexpressing transgenic materials, and these studies have not provided mechanistic insight into sHSP function or distinguished differences between CI and CII sHSPs.…”

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confidence: 99%

Class I and II small heat-shock proteins protect protein translation factors during heat stress

et al. 2016

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The ubiquitous small heat shock proteins (sHSPs) are well documented to act in vitro as molecular chaperones to prevent the irreversible aggregation of heat-sensitive proteins. However, the in vivo activities of sHSPs remain unclear. To investigate the two most abundant classes of plant cytosolic sHSPs (class I [CI] and class II [CII]), RNA interference (RNAi) and overexpression lines were created in Arabidopsis (Arabidopsis thaliana) and shown to have reduced and enhanced tolerance, respectively, to extreme heat stress. Affinity purification of CI and CII sHSPs from heat-stressed seedlings recovered eukaryotic translation elongation factor (eEF) 1B (a-, b-, and g-subunits) and eukaryotic translation initiation factor 4A (three isoforms), although the association with CI sHSPs was stronger and additional proteins involved in translation were recovered with CI sHSPs. eEF1B subunits became partially insoluble during heat stress and, in the CI and CII RNAi lines, showed reduced recovery to the soluble cell fraction after heat stress, which was also dependent on HSP101. Furthermore, after heat stress, CI sHSPs showed increased retention in the insoluble fraction in the CII RNAi line and vice versa. Immunolocalization revealed that both CI and CII sHSPs were present in cytosolic foci, some of which colocalized with HSP101 and with eEF1Bg and eEF1Bb. Thus, CI and CII sHSPs have both unique and overlapping functions and act either directly or indirectly to protect specific translation factors in cytosolic stress granules.

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confidence: 99%

Class I and II small heat-shock proteins protect protein translation factors during heat stress

et al. 2016

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“…The transcript was present in shoots as well as roots, however, the transcript abundance was higher in shoots as compared to roots. Changes in sHSPs expression are similar to those in LEA proteins at the time of dehydration and crucial developmental stages in Arabidopsis plants transformed with LimHSP16.45 (Mu et al 2013). Its strong expression in the guard cells, indicated that LimHSP16.45 probably regulated stomatal movement during times of drought.…”

Section: Expression Of Hsp In Brassica Juncea Under Drought Stressmentioning

confidence: 73%

Hsp transcript induction is correlated with physiological changes under drought stress in Indian mustard

Aneja

Yadav

Kumar

et al. 2015

Physiol Mol Biol Plants

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Brassica juncea is an important oilseed crop and drought stress is major abiotic stress that limits its growth and productivity. RH0116 (drought tolerant) and RH8812 (drought sensitive) genotypes were undertaken to study some of the physiological parameters and hsp gene expression related to stress tolerance under drought stress conditions. Differential response in terms of seed germination, electrolyte leakage, RWC, osmotic potential was observed in the selected genotypes. In vitro seed germination studies using PEG stress treatments indicated reduced seed germination with increasing levels of stress treatment. Electrolyte leakage increased, whereas, relative water content and osmotic potential decreased in stressed seedlings. Expression of hsp gene was found to be upregulated during drought stress as the transcripts were present only in the stressed plants and disappeared upon rehydration. The drought tolerant variety showed higher transcript accumulation as compared to the sensitive variety. The study showed that drought induced changes in gene expression in two contrasting genotypes were consistent with the physiological response.

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“…HSPs accumulate in diverse plant cells in response to even a short exposure to high temperature, and are normally synthesized to maintain homeostasis of plant processes during these conditions. HSPs maintain functional conformation of other proteins and moonlight as chaperones in the assembly and transport of nascent proteins, normally as well as in response to abiotic stresses [118,[128][129][130][131]. Additionally, engineering crop plants has resulted in resistance against various abiotic stresses and industry has successfully generated drought resistant germplasm for farmers.…”

Section: Improving Abiotic Stresses Tolerance In Crop Cultivarsmentioning

confidence: 99%

Sustainable Agriculture—Enhancing Environmental Benefits, Food Nutritional Quality and Building Crop Resilience to Abiotic and Biotic Stresses

Roberts¹,

Mattoo²

2018

Agriculture

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Feeding nutrition-dense food to future world populations presents agriculture with enormous challenges as estimates indicate that crop production must as much as double. Crop production cannot be increased to meet this challenge simply by increasing land acreage or using past agricultural intensification methods. Food production doubled in the past through substantial use of synthetic fertilizer, pesticides, and irrigation, all at significant environmental cost. Future production of nutrition-dense food will require next-generation crop production systems with decreased reliance on synthetic fertilizer and pesticide. Here, we present three case studies detailing the development of cover crops and plant-beneficial microbes for sustainable, next-generation small grain, tomato, and oilseed rape production systems. Cover crops imparted weed and pathogen control and decreased soil erosion and loss of soil nitrogen, phosphorus and carbon, while plant-beneficial microbes provided disease control and phosphorus fertility. However, yield in these next-generation crop production systems at best approximated that associated with current production systems. We argue here that to substantially increase agricultural productivity, new crop germplasm needs to be developed with enhanced nutritional content and enhanced tolerance to abiotic and biotic stress. This will require using all available technologies, including intensified genetic engineering tools, in the next-generation cropping systems.

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Overexpression of Small Heat Shock Protein LimHSP16.45 in Arabidopsis Enhances Tolerance to Abiotic Stresses

Cited by 90 publications

References 43 publications

Class I and II small heat-shock proteins protect protein translation factors during heat stress

Class I and II small heat-shock proteins protect protein translation factors during heat stress

Hsp transcript induction is correlated with physiological changes under drought stress in Indian mustard

Sustainable Agriculture—Enhancing Environmental Benefits, Food Nutritional Quality and Building Crop Resilience to Abiotic and Biotic Stresses

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