Hsp transcript induction is correlated with physiological changes under drought stress in Indian mustard

Aneja, Bharti; Yadav, N. R.; Kumar, Neeraj; Yadav, Renu

doi:10.1007/s12298-015-0305-3

Cited by 19 publications

(11 citation statements)

References 55 publications

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“…Increased expression of NADH ubiquinone oxidoreductase and induction of P450 genes were involved in resistance to pyriproxyfen insecticide in Bemisia tabaci [ 123 ]. Induction of heat shock proteins is associated with drought and oxidative stress in Brassica juncea [ 124 ] and A . thaliana [ 125 ], respectively.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq transcriptome analysis of Amaranthus palmeri with differential tolerance to glufosinate herbicide

Salas-Perez

Saski²,

Noorai³

et al. 2018

PLoS ONE

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Amaranthus palmeri (Amaranthaceae) is a noxious weed in several agroecosystems and in some cases seriously threatens the sustainability of crop production in North America. Glyphosate-resistant Amaranthus species are widespread, prompting the use of alternatives to glyphosate such as glufosinate, in conjunction with glufosinate-resistant crop cultivars, to help control glyphosate-resistant weeds. An experiment was conducted to analyze the transcriptome of A. palmeri plants that survived exposure to 0.55 kg ha-1 glufosinate. Since there was no record of glufosinate use at the collection site, survival of plants within the population are likely due to genetic expression that pre-dates selection; in the formal parlance of weed science this is described as natural tolerance. Leaf tissues from glufosinate-treated and non-treated seedlings were harvested 24 h after treatment (HAT) for RNA-Seq analysis. Global gene expression was measured using Illumina DNA sequence reads from non-treated and treated surviving (presumably tolerant, T) and susceptible (S) plants. The same plants were used to determine the mechanisms conferring differential tolerance to glufosinate. The S plants accumulated twice as much ammonia as did the T plants, 24 HAT. The relative copy number of the glufosinate target gene GS2 did not differ between T and S plants, with 1 to 3 GS2 copies in both biotypes. A reference cDNA transcriptome consisting of 72,780 contigs was assembled, with 65,282 sequences putatively annotated. Sequences of GS2 from the transcriptome assembly did not have polymorphisms unique to the tolerant plants. Five hundred sixty-seven genes were differentially expressed between treated T and S plants. Of the upregulated genes in treated T plants, 210 were more highly induced than were the upregulated genes in the treated S plants. Glufosinate-tolerant plants had greater induction of ABC transporter, glutathione S-transferase (GST), NAC transcription factor, nitronate monooxygenase (NMO), chitin elicitor receptor kinase (CERK1), heat shock protein 83, ethylene transcription factor, heat stress transcription factor, NADH-ubiquinone oxidoreductase, ABA 8’-hydroxylase, and cytochrome P450 genes (CYP72A, CYP94A1). Seven candidate genes were selected for validation using quantitative real time-PCR. While GST was upregulated in treated tolerant plants in at least one population, CYP72A219 was consistently highly expressed in all treated tolerant biotypes. These genes are candidates for contributing tolerance to glufosinate. Taken together, these results show that differential induction of stress-protection genes in a population can enable some individuals to survive herbicide application. Elevated expression of detoxification-related genes can get fixed in a population with sustained selection pressure, leading to evolution of resistance. Alternatively, sustained selection pressure could select for mutation(s) in the GS2 gene with the same consequence.

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Section: Discussionmentioning

confidence: 99%

RNA-Seq transcriptome analysis of Amaranthus palmeri with differential tolerance to glufosinate herbicide

Salas-Perez

Saski²,

Noorai³

et al. 2018

PLoS ONE

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“…vannamei , the HSPs were detected as the main expressed genes among the major immune factors, and two HSP70 family genes (HSPA8 and HSPA4) were discovered as the hub regulation genes in the PPI networks. The key role of HSP70s had been proved involving in response to various stressful stimuli in previous studies [124–125]. The results of Duan et al (2018) showed that the expression of HSP70 increased to the highest level by exposing the L .…”

Section: Discussionmentioning

confidence: 98%

Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress

Huang

Cheng

et al. 2018

PLoS ONE

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Soil salinization erodes the farmlands and poses a serious threat to human life, reuse of the saline-alkali lands as cultivated resources becomes increasingly prominent. Pacific white shrimp (Litopenaeus vannamei) is an important farmed aquatic species for the development and utilization of the saline-alkali areas. However, little is known about the adaptation mechanism of this species in terms of high-pH stress. In the present study, a transcriptome analysis on the gill tissues of L. vannamei in response to high-pH stress (pH 9.3 ± 0.1) was conducted. After analyzing, the cyclic nucleotide gated channel-Ca2+ (CNGC-Ca2+) and patched 1 (Ptc1) were detected as the majority annotated components in the cAMP signaling pathway (KO04024), indicating that the CNGC-Ca2+ and Ptc1 might be the candidate components for transducing and maintaining the high-pH stress signals, respectively. The immunoglobulin superfamily (IgSF), heat shock protein (HSP), glutathione s-transferase (GST), prophenoloxidase/phenoloxidase (proPO/PO), superoxide dismutase (SOD), anti-lipopolysaccharide factor (ALF) and lipoprotein were discovered as the major transcribed immune factors in response to high-pH stress. To further detect hub regulation-genes, protein-protein interaction (PPI) networks were constructed; the genes/proteins “Polymerase (RNA) II (DNA directed) polypeptide A” (POLR2A), “Histone acetyltransferase p300” (EP300) and “Heat shock 70kDa protein 8” (HSPA8) were suggested as the top three hub regulation-genes in response to acute high-pH stress; the genes/proteins “Heat shock 70kDa protein 4” (HSPA4), “FBJ murine osteosarcoma viral oncogene homolog” (FOS) and “Nucleoporin 54kDa” (NUP54) were proposed as the top three hub regulation-genes involved in adapting endurance high-pH stress; the protein-interactions of “EP300-HSPA8” and “HSPA4-NUP54” were detected as the most important biological interactions in response to the high-pH stress; and the HSP70 family genes might play essential roles in the adaptation of the high-pH stress environment in L. vannamei. These findings provide the first insight into the molecular and immune basis of L. vannamei in terms of high-pH environments, and the construction of a PPI network might improve our understanding in revealing the hub regulation-genes in response to abiotic stress in shrimp species and might be beneficial for further studies.

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“…The transcript levels of bHLH122 were strongly induced by drought, and up-regulation of bHLH122 substantially increased cellular ABA levels, which demonstrated that bHLH122 functions as a positive regulator of water-deficit conditions (Liu et al, 2014). Heat shock proteins (HSPs) are induced by drought and correspond to enhanced tolerance (Aneja et al, 2015). Previous studies found that overexpression of HSP17.6A in Arabidopsis resulted in gain of tolerance in these plants during water-deficit conditions, possibly through protein stabilization (Sun et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought Stress

Liu

et al. 2021

Front. Plant Sci.

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SMALL AUXIN UP-REGULATED RNAs (SAURs) are recognized as auxin-responsive genes involved in the regulation of abiotic stress adaptive growth. Among the growth-limiting factors, water-deficit condition significantly affects plant growth and development. The putative function of SAUR family member AtSAUR32 has the potential to diminish the negative impact of drought stress, but the exact function and mode of action remain unclear in Arabidopsis. In the current study, AtSAUR32 gene was cloned and functionally analyzed. AtSAUR32 localized to the plasma membrane and nucleus was dominantly expressed in roots and highly induced by abscisic acid and drought treatment at certain time points. The stomatal closure and seed germination of saur32 were less sensitive to ABA relative to AtSAUR32-overexpressed line (OE32-5) and wild type (WT). Moreover, the saur32 mutant under drought stress showed increased ion leakage while quantum yield of photosystem II (ΦPSII) and endogenous ABA accumulation were reduced, along with the expression pattern of ABA/stress-responsive genes compared with WT and the OE32-5 transgenic line. Additionally, yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that AtSAUR32 interacted with clade-A PP2C proteins (AtHAI1 and AtAIP1) to regulate ABA sensitivity in Arabidopsis. Taken together, these results indicate that AtSAUR32 plays an important role in drought stress adaptation via mediating ABA signal transduction.

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Hsp transcript induction is correlated with physiological changes under drought stress in Indian mustard

Cited by 19 publications

References 55 publications

RNA-Seq transcriptome analysis of Amaranthus palmeri with differential tolerance to glufosinate herbicide

RNA-Seq transcriptome analysis of Amaranthus palmeri with differential tolerance to glufosinate herbicide

Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress

The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought Stress

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