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Soil volumetric water content ( V W C ) is a vital parameter to understand several ecohydrological and environmental processes. Its cost-effective measurement can potentially drive various technological tools to promote data-driven sustainable agriculture through supplemental irrigation solutions, the lack of which has contributed to severe agricultural distress, particularly for smallholder farmers. The cost of commercially available V W C sensors varies over four orders of magnitude. A laboratory study characterizing and testing sensors from this wide range of cost categories, which is a prerequisite to explore their applicability for irrigation management, has not been conducted. Within this context, two low-cost capacitive sensors—SMEC300 and SM100—manufactured by Spectrum Technologies Inc. (Aurora, IL, USA), and two very low-cost resistive sensors—the Soil Hygrometer Detection Module Soil Moisture Sensor (YL100) by Electronicfans and the Generic Soil Moisture Sensor Module (YL69) by KitsGuru—were tested for performance in laboratory conditions. Each sensor was calibrated in different repacked soils, and tested to evaluate accuracy, precision and sensitivity to variations in temperature and salinity. The capacitive sensors were additionally tested for their performance in liquids of known dielectric constants, and a comparative analysis of the calibration equations developed in-house and provided by the manufacturer was carried out. The value for money of the sensors is reflected in their precision performance, i.e., the precision performance largely follows sensor costs. The other aspects of sensor performance do not necessarily follow sensor costs. The low-cost capacitive sensors were more accurate than manufacturer specifications, and could match the performance of the secondary standard sensor, after soil specific calibration. SMEC300 is accurate ( M A E , R M S E , and R A E of 2.12%, 2.88% and 0.28 respectively), precise, and performed well considering its price as well as multi-purpose sensing capabilities. The less-expensive SM100 sensor had a better accuracy ( M A E , R M S E , and R A E of 1.67%, 2.36% and 0.21 respectively) but poorer precision than the SMEC300. However, it was established as a robust, field ready, low-cost sensor due to its more consistent performance in soils (particularly the field soil) and superior performance in fluids. Both the capacitive sensors responded reasonably to variations in temperature and salinity conditions. Though the resistive sensors were less accurate and precise compared to the capacitive sensors, they performed well considering their cost category. The YL100 was more accurate ( M A E , R M S E , and R A E of 3.51%, 5.21% and 0.37 respectively) than YL69 ( M A E , R M S E , and R A E of 4.13%, 5.54%, and 0.41, respectively). However, YL69 outperformed YL100 in terms of precision, and response to temperature and salinity variations, to emerge as a more robust resistive sensor. These very low-cost sensors may be used in combination with more accurate sensors to better characterize the spatiotemporal variability of field scale soil moisture. The laboratory characterization conducted in this study is a prerequisite to estimate the effect of low- and very low-cost sensor measurements on the efficiency of soil moisture based irrigation scheduling systems.

Phytoremediation potential of six halophytic species i.e. Suaeda nudiflora, Suaeda fruticosa, Portulaca oleracea, Atriplex lentiformis, Parkinsonia aculeata and Xanthium strumarium was assessed under screen house conditions. Plants were raised at 8.0, 12.0, 16.0, and 20.0 dSm(-1) of chloride-dominated salinity. The control plants were irrigated with canal water. Sampling was done at vegetative stage (60-75 DAS). About 95 percent seed germination occurred up to 12 dSm(-1) and thereafter declined slightly. Mean plant height and dry weight plant(-1) were significantly decreased from 48.71 to 32.44 cm and from 1.73 to 0.61g plant(-1) respectively upon salinization. Na(+)/K(+) ratio (0.87 to 2.72), Na(+)/ Ca(2+) + Mg(2+) (0.48 to 1.54) and Cl(-)/SO4(2-) (0.94 to 5.04) ratio showed increasing trend. Salinity susceptibility index was found minimum in Suaeda fruticosa (0.72) and maximum in Parkinsonia aculeata (1.17). Total ionic content also declined and magnitude of decline varied from 8.51 to 18.91% at 8 dSm(-1) and 1.85 to 7.12% at 20 dSm(-1) of salinity. On the basis of phytoremediation potential Suaeda fruticosa (1170.02 mg plant(-1)), Atriplex lentiformis (777.87 mg plant(-1)) were the best salt hyperaccumulator plants whereas Xanthium strumarium (349.61 mg plant(-1)) and Parkinsonia aculeata (310.59 mg plant(-1)) were the least hyperaccumulator plants.

Root traits, such as depth and root biomass, have been identified as the most promising plant traits in chickpea for terminal drought tolerance. With this objective, five contrasting genotypes of chickpea, viz. ICCV-4958, H-208, HC-5, RSG-931 and CSJ-379, having wide adaptability to drought prone areas at national level were assessed for various root characteristics under two environments, i.e. irrigated and rain-fed. The sampling was done at full bloom stage and there were significant differences in the rooting depth among the genotypes both under irrigated and rain-fed conditions. The chickpea roots penetrated to a minimum depth of 92 cm in CSJ-379 and maximum of 122 cm in ICCV-4958 under rain-fed conditions. The rooting depth remained higher under rain-fed than irrigated environment. Under irrigated conditions, the chickpea roots were able to grow to a maximum depth of 99 and 97 cm in HC-5 and ICCV-4958, respectively. Among the genotypes, biomass per plant of the root was higher in ICCV-4958 (6.7 g) and HC-5 (5.6 g) under rain-fed conditions. Similar observations were recorded for root/shoot ratio, dry weights of stem, leaf, nodules and total dry weight per plant. The moisture stress increased the biomass partitioning towards the roots. The water potential (ψ w ), osmotic potential (ψ s ) and relative water content (RWC %) of leaf were -0.98 MPa, -1.82 MPa and 60%, respectively, in the genotype HC-5, and -1.02 MPa, -1.72 MPa and 64%, respectively, in ICCV-4958 under rain-fed conditions. The rates of photosynthesis, and transpiration, values of the stomatal conductance and photochemical efficiency/quantum yield as indicated by F v /F m ratio were in the range of 6.7 to 10.6 (μmol m −2 s −1 ), 1.27 to 2.38 (mmol m −2 s −1 ), 0.23 to 0.48 (mol m −2 s −1 ) and 0.457 to 0.584, respectively, under rain-fed conditions. Genotypes HC-5 and ICCV-4958 also maintained higher photosynthetic and transpiration rates and F v /F m ratio than others. The maximum F v /F m values in these genotypes were correlated with the higher photosynthetic rate and dry matter yield per plant. Relative stress injury (RSI %) values in HC-5 and ICCV-4958 noticed were 25.3% and 23.7%, respectively. The results of this study indicate that under rain-fed conditions, genotypes ICCV-4958 and HC-5 had higher dry weight of stem, leaves, roots, nodules and total dry weight per plant, rooting depth, root/shoot ratio, photosynthetic and transpiration rates, photochemical efficiency and better plant water status but lower stomatal conductance than other genotypes. These traits are directly associated with maximum seed yield per plant, i.e. 15.6 g and 14.7 g per plant, respectively, in these genotypes. Therefore, both the genotypes in future can be used in crop improvement programme of chickpea breeding for drought tolerance.

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Sclerotinia stem rot is one of the utmost important disease of mustard, causing considerable losses in seed yield and oil quality. The study of the genetic and proteomic basis of resistance to this disease is imperative for its effective utilization in developing resistant cultivars. Therefore, the genetic pattern of Sclerotinia stem rot resistance in Indian mustard was studied using six generations (P1, P2, F1, F2, BC1P1, and BC1P2) developed from the crossing of one resistant (RH 1222-28) and two susceptible (EC 766300 and EC 766123) genotypes. Genetic analysis revealed that resistance was governed by duplicate epistasis. Comparative proteome analysis of resistant and susceptible genotypes indicated that peptidyl-prolyl cis-trans isomerase (A0A078IDN6 PPIase) showed high expression in resistant genotype at the early infection stage while its expression was delayed in susceptible genotypes. This study provides important insight to mustard breeders for designing effective breeding programs to develop resistant cultivars against this devastating disease.