Overexpression of OsHSP18.0-CI Enhances Resistance to Bacterial Leaf Streak in Rice

Ju, Yanhu; Tian, Hongjuan; Zhang, Ruihua; Zuo, Liping; Jin, Guixiu; Xu, Qian; Ding, Xinhua; Li, Xiangkui; Zhang, Chu

doi:10.1186/s12284-017-0153-6

Cited by 46 publications

(47 citation statements)

References 43 publications

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“…Compared with traditional NGS technologies, this technology accomplishes single molecule real-time (SMRT) isoform sequencing (Iso-Seq) with long read lengths, uniform coverage, and high accuracy, which renders PacBio RSII very effective at capturing the full catalogue of transcripts and constructs of a comprehensive transcriptome for species without genome sequence [25]. Furthermore, RNA-Seq based on the BGISEQ-500 platform has been applied to gene expression comparisons of different species, developmental stages, and stresses [26, 27]. Currently, to our knowledge, genome-wide transcriptomic analysis of the salinity-responsive genes has not been reported in alfalfa root tips, where is the primary site for the perception of hyperosmotic pressure and ion toxicity [28, 29].…”

Section: Introductionmentioning

confidence: 99%

Full-length transcript sequencing and comparative transcriptomic analysis to evaluate the contribution of osmotic and ionic stress components towards salinity tolerance in the roots of cultivated alfalfa (Medicago sativa L.)

et al. 2019

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BackgroundAlfalfa is the most extensively cultivated forage legume. Salinity is a major environmental factor that impacts on alfalfa’s productivity. However, little is known about the molecular mechanisms underlying alfalfa responses to salinity, especially the relative contribution of the two important components of osmotic and ionic stress.ResultsIn this study, we constructed the first full-length transcriptome database for alfalfa root tips under continuous NaCl and mannitol treatments for 1, 3, 6, 12, and 24 h (three biological replicates for each time points, including the control group) via PacBio Iso-Seq. This resulted in the identification of 52,787 full-length transcripts, with an average length of 2551 bp. Global transcriptional changes in the same 33 stressed samples were then analyzed via BGISEQ-500 RNA-Seq. Totals of 8861 NaCl-regulated and 8016 mannitol-regulated differentially expressed genes (DEGs) were identified. Metabolic analyses revealed that these DEGs overlapped or diverged in the cascades of molecular networks involved in signal perception, signal transduction, transcriptional regulation, and antioxidative defense. Notably, several well characterized signalling pathways, such as CDPK, MAPK, CIPK, and PYL-PP2C-SnRK2, were shown to be involved in osmotic stress, while the SOS core pathway was activated by ionic stress. Moreover, the physiological shifts of catalase and peroxidase activity, glutathione and proline content were in accordance with dynamic transcript profiles of the relevant genes, indicating that antioxidative defense system plays critical roles in response to salinity stress.ConclusionsOverall, our study provides evidence that the response to salinity stress in alfalfa includes both osmotic and ionic components. The key osmotic and ionic stress-related genes are candidates for future studies as potential targets to improve resistance to salinity stress via genetic engineering.Electronic supplementary materialThe online version of this article (10.1186/s12870-019-1630-4) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Full-length transcript sequencing and comparative transcriptomic analysis to evaluate the contribution of osmotic and ionic stress components towards salinity tolerance in the roots of cultivated alfalfa (Medicago sativa L.)

et al. 2019

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“…It has been observed that in maize and other plants, different HSPs express abundantly during pollen development ( Magnard et al , 1996 ; Young et al , 2001 ; Kumar et al , 2015 ). Furthermore, overexpression of sHSPs in plants enhance their tolerance to abiotic stresses ( Ahn and Zimmerman, 2006 ; Maimbo et al , 2007 ; Jiang et al , 2009 ; Chauhan et al , 2012 ), whereas plants with reduced expression of sHSP exhibit compromised tolerance to heat resistance ( Charng et al , 2006 ) and disease ( Maimbo et al , 2007 ; Ju et al , 2017 ).…”

Section: Introductionmentioning

confidence: 99%

A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis

Zhang

Gao

et al. 2018

Genet. Mol. Biol.

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We cloned and characterized the full-length coding sequence of a small heat shock (sHSP) gene, PfHSP17.2, from Primula forrestii leaves following heat stress treatment. Homology and phylogenetic analysis suggested that PfHSP17.2 is a cytosolic class II sHSP, which was further supported by the cytosolic localization of transient expression of PfHSP17.2 fused with green fluorescent protein reporter. Expression analysis showed that PfHSP17.2 was highly inducible by heat stress in almost all the vegetative and generative tissues and was expressed under salt, cold, and oxidative stress conditions as well. Moreover, the expression of PfHSP17.2 in P. forrestii was detected in certain developmental growth stages. Transgenic Arabidopsis thaliana constitutively expressing PfHSP17.2 displayed increased thermotolerance and higher resistance to salt and cold compared with wild type plants. It is suggested that PfHSP17.2 plays a key role in heat and other abiotic stresses.

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“…However, no MR gene against Xoc is identified in rice until now [2], only some cell wall-related genes are associated with rice resistance to Xoc. The overexpression of polygalacturonase-inhibiting protein 1/4 (OsPGIP1/4) and small heat shock protein (OsHsp18.0-Cl) genes enhance resistance to bacterial leaf streak in rice [34][35][36]. The polygalacturonase-inhibiting protein functions in inhibiting the activity of enzymes which secreted by bacteria to degrade cell wall component-pectic polysaccharides [34].…”

Section: Discussionmentioning

confidence: 99%

Different Cell Wall-Degradation Ability Leads to Tissue-Specificity between Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola

et al. 2020

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Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) lead to the devastating rice bacterial diseases and have a very close genetic relationship. There are tissue-specificity differences between Xoo and Xoc, i.e., Xoo only proliferating in xylem vessels and Xoc spreading in intercellular space of mesophyll cell. But there is little known about the determinants of tissue-specificity between Xoo and Xoc. Here we show that Xoc can spread in the intercellular spaces of mesophyll cells to form streak lesions. But Xoo is restricted to growth in the intercellular spaces of mesophyll cells on the inoculation sites. In vivo, Xoc largely breaks the surface and inner structures of cell wall in mesophyll cells in comparison with Xoo. In vitro, Xoc strongly damages the cellulose filter paper in comparison with Xoo. These results suggest that the stronger cell wall-degradation ability of Xoc than that of Xoo may be directly determining the tissue-specificity.

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Overexpression of OsHSP18.0-CI Enhances Resistance to Bacterial Leaf Streak in Rice

Cited by 46 publications

References 43 publications

Full-length transcript sequencing and comparative transcriptomic analysis to evaluate the contribution of osmotic and ionic stress components towards salinity tolerance in the roots of cultivated alfalfa (Medicago sativa L.)

Full-length transcript sequencing and comparative transcriptomic analysis to evaluate the contribution of osmotic and ionic stress components towards salinity tolerance in the roots of cultivated alfalfa (Medicago sativa L.)

A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis

Different Cell Wall-Degradation Ability Leads to Tissue-Specificity between Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola

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