Overexpression of EcDREB2A transcription factor from finger millet in tobacco enhances tolerance to heat stress through ROS scavenging

Singh, Sonam; Chopperla, Ramakrishna; Shingote, Prashant Raghunath; Chhapekar, Sushil Satish; Deshmukh, Rupesh; Khan, Suphiya; Padaria, Jasdeep Chatrath; Sharma, Tilak Raj; Solanke, Amolkumar U.

doi:10.1016/j.jbiotec.2021.06.013

Cited by 23 publications

(3 citation statements)

References 81 publications

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“…Here, 2691 DEGs were identified, including 1809 upregulated and 882 downregulated. Further analysis demonstrated that most of the DEGs induced by heat stress were related to the HSF–HSP pathway, ROS scavenging enzymes, plant hormone signaling transduction, and TFs, including bZIP, MYB, NAC, WRKY, and bHLH, which have been reported in previous studies [ 25 , 27 , 47 , 48 ]. This study is the first report to investigate the transcriptomic profiles of C. tangshen in response to heat stress.…”

Section: Discussionsupporting

confidence: 67%

Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Jiang

Wassie

Zhou

et al. 2023

Life

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Codonopsis tangshen Oliv (C. tangshen) is a valuable traditional Chinese medicinal herb with tremendous health benefits. However, the growth and development of C. tangshen are seriously affected by high temperatures. Therefore, understanding the molecular responses of C. tangshen to high-temperature stress is imperative to improve its thermotolerance. Here, RNA-Seq analysis was performed to investigate the genome-wide transcriptional changes in C. tangshen in response to short-term heat stress. Heat stress significantly damages membrane stability and chlorophyll biosynthesis in C. tangshen, as evidenced by pronounced malonaldehyde (MDA), electrolyte leakage (EL), and reduced chlorophyll content. Transcriptome analysis showed that 2691 differentially expressed genes (DEGs) were identified, including 1809 upregulated and 882 downregulated. Functional annotations revealed that the DEGs were mainly related to heat shock proteins (HSPs), ROS-scavenging enzymes, calcium-dependent protein kinases (CDPK), HSP-HSP network, hormone signaling transduction pathway, and transcription factors such as bHLHs, bZIPs, MYBs, WRKYs, and NACs. These heat-responsive candidate genes and TFs could significantly regulate heat stress tolerance in C. tangshen. Overall, this study could provide new insights for understanding the underlying molecular mechanisms of thermotolerance in C. tangshen.

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Section: Discussionsupporting

confidence: 67%

Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Jiang

Wassie

Zhou

et al. 2023

Life

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“…Although DREB transcription factors were first reported to be involved in plant response to drought and cold stresses, some research indicated that DREBs and HSFs could interact with each other in response to extreme heat ( Grover et al., 2013 ). Recently Singh et al. (2021) showed that overexpression of EcDREB2A in tobacco improves heat stress tolerance through increasing ROS scavenging capacity in transgenic plants.…”

Section: Manipulation Of Single Genes In Plants Via ...mentioning

confidence: 99%

Genetic manipulation for abiotic stress resistance traits in crops

2022

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Abiotic stresses are major limiting factors that pose severe threats to agricultural production. Conventional breeding has significantly improved crop productivity in the last century, but traditional breeding has reached its maximum capacity due to the multigenic nature of abiotic stresses. Alternatively, biotechnological approaches could provide new opportunities for producing crops that can adapt to the fast-changing environment and still produce high yields under severe environmental stress conditions. Many stress-related genes have been identified and manipulated to generate stress-tolerant plants in the past decades, which could lead to further increase in food production in most countries of the world. This review focuses on the recent progress in using transgenic technology and gene editing technology to improve abiotic stress tolerance in plants, and highlights the potential of using genetic engineering to secure food and fiber supply in a world with an increasing population yet decreasing land and water availability for food production and fast-changing climate that will be largely hostile to agriculture.

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“…ZmWRKY106 , ERF74 and MYB44 activated antioxidant enzymes, and play an important role in scavenging ROS under heat stress on maize, Arabidopsis and Xanthoceras sorbifolium [ 64 , 69 , 110 ]. Singh et al reported that under heat stress, EcDREB2A overexpression also resulted in increased antioxidant enzymes activity with decreased ROS content in tobacco [ 111 ]. Heat shock transcription factors (HSFs) regulate the expression levels of heat shock proteins and play an important role in plant high temperature stress.…”

Section: The Function Of Transcription Factors In Ros Metabolism Unde...mentioning

confidence: 99%

Review of the Mechanisms by Which Transcription Factors and Exogenous Substances Regulate ROS Metabolism under Abiotic Stress

Liu

Gong

et al. 2022

Antioxidants

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Reactive oxygen species (ROS) are signaling molecules that regulate many biological processes in plants. However, excess ROS induced by biotic and abiotic stresses can destroy biological macromolecules and cause oxidative damage to plants. As the global environment continues to deteriorate, plants inevitably experience abiotic stress. Therefore, in-depth exploration of ROS metabolism and an improved understanding of its regulatory mechanisms are of great importance for regulating cultivated plant growth and developing cultivars that are resilient to abiotic stresses. This review presents current research on the generation and scavenging of ROS in plants and summarizes recent progress in elucidating transcription factor-mediated regulation of ROS metabolism. Most importantly, the effects of applying exogenous substances on ROS metabolism and the potential regulatory mechanisms at play under abiotic stress are summarized. Given the important role of ROS in plants and other organisms, our findings provide insights for optimizing cultivation patterns and for improving plant stress tolerance and growth regulation.

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Overexpression of EcDREB2A transcription factor from finger millet in tobacco enhances tolerance to heat stress through ROS scavenging

Cited by 23 publications

References 81 publications

Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Genetic manipulation for abiotic stress resistance traits in crops

Review of the Mechanisms by Which Transcription Factors and Exogenous Substances Regulate ROS Metabolism under Abiotic Stress

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