Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Jiang, Xiaogang; Wassie, Misganaw; Zhou, Wei; Wang, Hua; You, Jinwen; Ma, Guangjing; Zhang, Meide

doi:10.3390/life13010168

Cited by 3 publications

(2 citation statements)

References 79 publications

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“…Similarly, our results demonstrated that chlorophyll (Chl a, Chl b) content declined after continuous cropping. One study showed the level of antioxidant enzymes SOD and the stress marker malonaldehyde (MDA) in the cells reflected the degree of oxidative damage [ 16 , 19 ]. In the present study, LZ significantly reduced the SOD activity and enhanced the MDA content, suggesting that the reduced SOD activity causing oxidative damage was indicated by increased malonaldehyde (MDA) contents.…”

Section: Discussionmentioning

confidence: 99%

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TMT-Based Proteomic Analysis of Continuous Cropping Response in Codonopsis tangshen Oliv.

Jiang

Zhou

Wang

et al. 2023

Life

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The growth and development of Codonopsis tangshen, an important herb used in Chinese traditional medicine, have been seriously affected by continuous cropping obstacles. Therefore, understanding the molecular responses of C. tangshen to continuous cropping is imperative to improve its resistance to continuous cropping obstacles. Here, physiological and biochemical results showed that the levels of chlorophyll and malonaldehyde (MDA) were higher in the continuous cropping (LZ) group compared with those of the non-continuous cropping (FLZ) group, while superoxide dismutase (SOD) content was lower in the LZ group than in the FLZ group. Tandem mass tag (TMT)-based proteomic analysis was performed to investigate the response mechanism to continuous cropping obstacles in C. tangshen. A total of 70 differentially expressed proteins (DEPs) were significantly involved in relevant pathways, including photosynthesis, oxidative phosphorylation, ribosome activity, and secondary metabolites. The results suggest that these DEPs in C. tangshen might play a critical role in response to continuous cropping. These findings could provide scientific basis for improving C. tangshen’s resistance to continuous cropping obstacles.

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Section: Discussionmentioning

confidence: 99%

“…The primers used in this study are listed in Table S1 . The 2 −ΔΔCT method was used to determine the relative expression of genes [ 16 ]. One-way ANOVA was applied to analyze the statistical differences between different groups.…”

Section: Methodsmentioning

confidence: 99%

TMT-Based Proteomic Analysis of Continuous Cropping Response in Codonopsis tangshen Oliv.

Jiang

Zhou

Wang

et al. 2023

Life

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Combined transcriptome and metabolome analyses reveal the effects of selenium on the growth and quality of Lilium lancifolium

Jiang,

Zhou,

et al. 2024

Front. Plant Sci.

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Lilium lancifolium Thunb (L. lancifolium) is an important medicinal and edible plant with outstanding functionality for selenium (Se) biofortification. However, the molecular response of L. lancifolium to exogenous Se has not been fully elucidated. In this study, the effects of different levels of Se on L. lancifolium growth and quality were explored by transcriptome, metabolome and biochemical analyses. The results showed that the total Se and organic Se content in L. lancifolium bulbs increased with increasing Se dosage (0–8.0 mmol/L). Moreover, Se stimulated the growth of L. lancifolium at low level (2.0 mmol/L) but showed an inhibitory effect at high levels (≥4.0 mmol/L). Metabolomic and biochemical analyses revealed that the bulb weight and the content of amino acid, soluble sugar, and soluble protein were significantly increased in the 2.0 mmol/L Se treatment compared with those in the control (0 mmol/L Se). Transcriptome and metabolome analyses revealed that the significant upregulation of the GPD1, GPAT and ADPRM genes promoted glycerophospholipid accumulation. Additionally, the significantly upregulated glyA and downregulated asnB, nadB, thrA and SAT genes coordinate to the regulation of amino acid biosynthesis. The significantly upregulated SUS, bgl B, BAM, and SGA1 genes were involved in soluble sugar accumulation under Se treatment. In summary, this study identified the optimal Se concentration (2.0 mmol/L), which significantly improved the growth and nutritional quality of L. lancifolium and contributed to understanding the combined effects of Se treatment on the expression of genes and the accumulation of metabolites in L. lancifolium bulbs.

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Research Progress on Heat Stress Response Mechanism and Control Measures in Medicinal Plants

Zhu,

Bao,

Yang

et al. 2024

IJMS

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Medicinal plants play a pivotal role in traditional medicine and modern pharmacology due to their various bioactive compounds. However, heat stress caused by climate change will seriously affect the survival and quality of medicinal plants. In this review, we update our understanding of the research progress on medicinal plants’ response mechanisms and control measures under heat stress over the last decade. This includes physiological changes, molecular mechanisms, and technical means to improve the heat tolerance of medicinal plants under heat stress. It provides a reference for cultivating heat-resistant varieties of medicinal plants and the rational utilization of control measures to improve the heat resistance of medicinal plants.

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Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen

Cited by 3 publications

References 79 publications

TMT-Based Proteomic Analysis of Continuous Cropping Response in Codonopsis tangshen Oliv.

TMT-Based Proteomic Analysis of Continuous Cropping Response in Codonopsis tangshen Oliv.

Combined transcriptome and metabolome analyses reveal the effects of selenium on the growth and quality of Lilium lancifolium

Research Progress on Heat Stress Response Mechanism and Control Measures in Medicinal Plants

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