Overexpression of Arabidopsis WRI1 enhanced seed mass and storage oil content in Camelina sativa

An, Dahee; Suh, Mi Chung

doi:10.1007/s11816-015-0351-x

Cited by 79 publications

(58 citation statements)

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“…This is in accordance with the study where B. napus WRI1 was overexpressed in Arabidopsis , leading to seed oil content increases of 10–40% (Liu et al, 2010) and the study by An and Suh (2015) mentioned above.…”

Section: Discussionsupporting

confidence: 92%

“…Our results showed that overexpression of the AtWRI1, AtHb2 , and BvHb2 gene could increase the oil content up to 29.9, 20.2, and 25.9%, respectively in field cress. A similar increase has been demonstrated in camelina, where a seed-specific overexpression of AtWRI1 yielded a 14% increase in total seed oil content (An and Suh, 2015). An even higher increase has been shown in maize where ZmWRI1 -overexpression generated an average increase in seed oil content of 30.6% (Shen et al, 2010).…”

Section: Discussionsupporting

confidence: 73%

“…Several attempts to alter the oil content of various crops have been made, both by upregulation of the key enzymes in the oil biosynthesis (Jako et al, 2001; Lardizabal et al, 2008; Liu et al, 2015) and regulations of some important transcription factors involved in the oil biosynthesis (Liu et al, 2010; Shen et al, 2010; An and Suh, 2015). For instance, overexpression of DGAT in Arabidopsis yielded seed oil content increases of between 9 and 12% on a dry weight basis (Jako et al, 2001), while the seed oil increases ranging between 10 and 40% was generated by BnWRI1- overexpression in Arabidopsis (Liu et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Seed weight increases have been reported in transgenic Arabidopsis lines expressing BnWRI1 (Liu et al, 2010), and in AtWRI1- expressing lines of camelina (An and Suh, 2015). In this study, significant increases in seed weight were also observed in two of the third generation field cress lines overexpressing AtWRI1 .…”

Section: Discussionmentioning

confidence: 99%

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Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre

et al. 2017

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The wild species field cress (Lepidium campestre), belonging to the Brassicaceae family, has potential to be developed into a novel oilseed- and catch crop, however, the species needs to be further improved regarding some important agronomic traits. One of them is its low oil content which needs to be increased. As far as we know there is no study aiming at increasing the oil content that has been reported in this species. In order to investigate the possibility to increase the seed oil content in field cress, we have tried to introduce the Arabidopsis WRINKLED1 (AtWRI1) or hemoglobin (Hb) genes from either Arabidopsis thaliana (AtHb2) or Beta vulgaris (BvHb2) into field cress with the seed specific expression. The hypothesis was that the oil content would be increased by overexpressing these target genes. The results showed that the oil content was indeed increased by up to 29.9, 20.2, and 25.9% in the transgenic lines expressing AtWRI1, AtHb2, and BvHb2, respectively. The seed oil composition of the transgenic lines did not significantly deviate from the seed oil composition of the wild type plants. Our results indicate that genetic modification can be used in this wild species for its fast domestication into a future economically viable oilseed and catch crop.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre

et al. 2017

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“…WRINKLED1 (WRI1) was shown to be an essential transcription factor for TAG synthesis in many species, and its overexpression led to 10-30% increase in seed oil content in arabidopsis, rapeseed and even maize (Cernac and Benning, 2004;Baud et al, 2007;Liu et al, 2010;Pouvreau et al, 2011;Wu et al, 2014). Overexpression of arabidopsis AtWRI1 in camelina seeds led to an increase in seed weight and seed size, and as expected, to 8-31% increase in seed oil content (An and Suh, 2015). Although camelina already has three copies of endogenous WRI1, it is remarkable that significant yield gain could be achieved by overexpression of an orthologous gene.…”

Section: Improving Camelina Oil Yieldmentioning

confidence: 52%

Camelina, a Swiss knife for plant lipid biotechnology

Faure

Tepfer

2016

OCL

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-Camelina has emerged in the last decade as a multipurpose crop plant particularly suitable for engineering new lipids for diverse uses, including feed, biofuel and green chemistry. The rebirth of this ancient crop was based on several intrinsic favorable characteristics: robust agronomic qualities, attractive oil profile, genetic proximity with the model plant arabidopsis, ease of genetic transformation by floral dip. The need to increase both the production and diversity of plant oils, while improving the sustainability of agricultural systems, has been the driving forces behind the ever-increasing investment in camelina research. Worldwide interest in engineering camelina has led to the development of a remarkable pipeline that allows the rapid production and phenotyping of new lines; it includes specific tools, such as databases, collections of natural accessions, methods of genetic transformation and lipid analysis. Implementation of numerous metabolic pathways in camelina for the production of novel lipids has highlighted the potential as well as the versatility of this new "old" oilseed crop that is well on the way to becoming an ideal plant chassis for lipid synthetic biology.Keywords: Lipid metabolism / oilseed / omega-3 / biofuels Résumé -La cameline, véritable couteau-suisse pour la biotechnologie végétale des lipides. La caméline a émergé durant la dernière décennie comme une espèce agronomique polyvalente particulièrement adaptée à l'ingé-nierie des lipides pour divers usages comme les biocarburants, l'alimentation ou la chimie verte. La renaissance de cette espèce cultivée ancienne est fondée sur plusieurs caractéristiques : robustesse agronomique, profil en huile attractif, proximité génétique avec la plante modèle arabidopsis et très grande facilité de transformation génétique par infiltration des fleurs. Le besoin d'augmenter la production d'huile végétale tout en diversifiant la nature des lipides produits, associé à l'amélioration de la durabilité des systèmes de production, ont été les déterminants qui ont poussé le développement de la recherche autour de la caméline. L'intérêt de par le monde pour l'ingénierie des lipides chez la caméline a ainsi permis la mise en place d'un ensemble d'outils incluant des bases de données, des collections d'accessions naturelles, du matériel spécifique pour la transformation et le phénotypage, ainsi que des méthodes pour la production et la caractérisation de nouvelles variétés ou de lignées. Les nombreux exemples de mise en place de nouvelles voies métaboliques chez la caméline pour la production de lipides spécifiques illustrent le potentiel important de cette nouvelle « ancienne » espèce oleogineuse qui est en passe de devenir un chassis végétal idéal pour la biologie synthétique des lipides. Mots clés :Métabolisme lipidique / oléagineux / oméga-3 / biocarburants

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The Survey of Genetic Engineering Approaches for Oil/Fatty Acid Content Improvement in Oilseed Crops

Bhati

Riyazuddin

Pathak

et al. 2021

Genome Engineering for Crop Improvement

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Overexpression of Arabidopsis WRI1 enhanced seed mass and storage oil content in Camelina sativa

Cited by 79 publications

References 64 publications

Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre

Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre

Camelina, a Swiss knife for plant lipid biotechnology

The Survey of Genetic Engineering Approaches for Oil/Fatty Acid Content Improvement in Oilseed Crops

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