Camelina, a Swiss knife for plant lipid biotechnology

Faure, Jean-Denis; Tepfer, Mark

doi:10.1051/ocl/2016023

Cited by 31 publications

(31 citation statements)

References 77 publications

(81 reference statements)

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“…In particular, it provides a very efficient method for selecting an ideotype (oil profile), minimizing negative effects (growth trade-off), by selecting the nature of the alleles and their most efficient genetic combinations. In the case of ancient crops that have undergone little breeding improvement such as Camelina, CRISPR-Cas9 genome editing provides major genetic leverage to improve its growing agronomical and biotechnological potential (Faure and Tepfer, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa

Morineau

Bellec

Tellier

et al. 2017

Plant Biotechnology Journal

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233

166

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In many plant species, gene dosage is an important cause of phenotype variation. Engineering gene dosage, particularly in polyploid genomes, would provide an efficient tool for plant breeding. The hexaploid oilseed crop Camelina sativa, which has three closely related expressed subgenomes, is an ideal species for investigation of the possibility of creating a large collection of combinatorial mutants. Selective, targeted mutagenesis of the three delta‐12‐desaturase (FAD2) genes was achieved by CRISPR‐Cas9 gene editing, leading to reduced levels of polyunsaturated fatty acids and increased accumulation of oleic acid in the oil. Analysis of mutations over four generations demonstrated the presence of a large variety of heritable mutations in the three isologous CsFAD2 genes. The different combinations of single, double and triple mutants in the T3 generation were isolated, and the complete loss‐of‐function mutants revealed the importance of delta‐12‐desaturation for Camelina development. Combinatorial association of different alleles for the three FAD2 loci provided a large diversity of Camelina lines with various lipid profiles, ranging from 10% to 62% oleic acid accumulation in the oil. The different allelic combinations allowed an unbiased analysis of gene dosage and function in this hexaploid species, but also provided a unique source of genetic variability for plant breeding.

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Section: Discussionmentioning

confidence: 99%

“…The ratio of 18 : 1/ (18 : 0 + 18 : 1 + 18 : 2 + 18 : 3)*100 was calculated to define the oleic acid index (OAI). Oil was extracted by a home-made centrifugation-based micropress device that can efficiently separate oil fraction from residual seed cake (Faure and Tepfer, 2016).…”

Section: Lipid Analysismentioning

confidence: 99%

Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa

Morineau

Bellec

Tellier

et al. 2017

Plant Biotechnology Journal

Self Cite

233

166

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show abstract

“…It is also a low-input, relatively stress-tolerant plant with a fast lifecycle (3 months from seed to seed) and is amenable to genetic transformation by the floral dip method. The hexaploid genome of Camelina has been sequenced and typically encodes three copies of each of the corresponding Arabidopsis genes, with which it shows an extremely high degree (.90%) of sequence similarity (Gehringer et al, 2006;Lu and Kang, 2008;Nguyen et al, 2013;Kagale et al, 2014;Faure and Tepfer, 2016).…”

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confidence: 99%

The Role of Gβ Protein in Controlling Cell Expansion via Potential Interaction with Lipid Metabolic Pathways

2019

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Heterotrimeric G-proteins influence almost all aspects of plant growth, development, and responses to biotic and abiotic stresses in plants, likely via their interaction with specific effectors. However, the identity of such effectors and their mechanism of action are mostly unknown. While investigating the roles of different G-protein subunits in modulating the oil content in Camelina (Camelina sativa), an oil seed crop, we uncovered a role of Gb proteins in controlling anisotropic cell expansion. Knockdown of Gb genes causes reduced longitudinal and enhanced transverse expansion, resulting in altered cell, tissue, and organ shapes in transgenic plants during vegetative and reproductive development. These plants also exhibited substantial changes in their fatty acid and phospholipid profiles, which possibly leads to the increased oil content of the transgenic seeds. This increase is potentially caused by the direct interaction of Gb proteins with a specific patatin-like phospholipase, pPLAIIId. Camelina plants with suppressed Gb expression exhibit higher lipase activity, and show phenotypes similar to plants overexpressing pPLAIIId, suggesting that the Gb proteins are negative regulators of pPLAIIId. These results reveal interactions between the G-protein-mediated and lipid signaling/metabolic pathways, where specific phospholipases may act as effectors that control key developmental and environmental responses of plants.

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“…short lifecycle, remarkable adaptation to a wide range of climatic conditions, etc. ), its relatively low oil yield (compared to other oilseed plants) represents a real limitation to it using in chemical and/or pharmaceutical industries (Faure and Tepfer 2015). Improving oil yield is therefore a priority for the development of this crop for large-scale use.…”

Section: Introductionmentioning

confidence: 99%

Targeting transgene to seed resulted in high rate of morphological abnormalities of Camelina transformants

Šutá

Matušíková

Blehová

2019

Nova Biotechnologica Et Chimica

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False flax (Camelina sativa L.) is currently under-exploited but highly promising oilseed crop. Combining Camelina's attractive agronomic traits with its unprecedented ease for genetic engineering makes it an ideal plant chassis for biotechnology applications, in particular synthetic biology strategies. For targeted expression of transgene particularly to seeds requires identification and application of seed specific promoters. In the present study two cultivars of Camelina, namely Zuzana and Smilowska, were used for transformation at early flowering stage using the floral dip method. The plants were inoculated with Agrobacterium bearing a construct for expression of red fluorescent protein (RFP) under the control of the seed specific cruciferin promoter CRUC from Arabidopsis. Transgenic seeds and plants were identified on the basis of red fluorescence (RFP) and kanamycin resistance. Relatively high transformation efficiency of 8 % was achieved particularly for the cultivar Zuzana. However, many of regenerants exerted developmental deformations such as lack of shoot apical meristem, deformed or absent cotyledons, etc. Furthermore, the activity of the CRUC promoter was still active also in true leaves rendering this promoter as inappropriate for seed targeting of the transgene. Nevertheless, genetic transformation remains a tool for direct modulation of pathways for oil synthesis in oilseed crops.

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Camelina, a Swiss knife for plant lipid biotechnology

Cited by 31 publications

References 77 publications

Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa

Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa

The Role of Gβ Protein in Controlling Cell Expansion via Potential Interaction with Lipid Metabolic Pathways

Targeting transgene to seed resulted in high rate of morphological abnormalities of Camelina transformants

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