The Survey of Genetic Engineering Approaches for Oil/Fatty Acid Content Improvement in Oilseed Crops

Bhati, Kaushal Kumar; Riyazuddin, Riyazuddin; Pathak, Ashish; Singh, Anuradha

doi:10.1002/9781119672425.ch11

Cited by 4 publications

(5 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Soybean seeds contain approximately 40% protein and 20% oil ( Chaudhary et al., 2015 ; Li et al., 2015 ). Optimizing carbon flux towards the synthesis of fatty acids (FAs) and amino acids and improving seed quality has always been a major objective of soybean breeding programs ( Bhati et al., 2021 ). However, the negative correlations between seed protein content with seed oil content and seed yield have hindered progress ( Chung et al., 2003 ; Patil et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean

Wang

Zhang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Soybean [Glycine max (L.) Merri.] is one of the most valuable global crops. And vegetable soybean, as a special type of soybean, provides rich nutrition in people’s life. In order to investigate the gene expression networks and molecular regulatory mechanisms that regulate soybean seed oil and protein contents during seed development, we performed transcriptomic and metabolomic analyses of soybean seeds during development in two soybean varieties that differ in protein and oil contents. We identified a total of 41,036 genes and 392 metabolites, of which 12,712 DEGs and 315 DAMs were identified. Analysis of KEGG enrichment demonstrated that DEGs were primarily enriched in phenylpropanoid biosynthesis, glycerolipid metabolism, carbon metabolism, plant hormone signal transduction, linoleic acid metabolism, and the biosynthesis of amino acids and secondary metabolites. K-means analysis divided the DEGs into 12 distinct clusters. We identified candidate gene sets that regulate the biosynthesis of protein and oil in soybean seeds, and present potential regulatory patterns that high seed-protein varieties may be more sensitive to desiccation, show earlier photomorphogenesis and delayed leaf senescence, and thus accumulate higher protein contents than high-oil varieties.

show abstract

Section: Introductionmentioning

confidence: 99%

Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean

Wang

Zhang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…In this context, bioengineered cell factories of oilseed species to produce high oleic acid along with other valuable molecules can be an attractive, renewable, and sustainable manner alternative platform to plant cultivation (Bhati et al, 2021). GM approaches could also be directed to improve the efficiency of WT callus cultures as a potential source for plant oils (Chandran et al, 2020), since it was observed, for example, in Jatropha curcas, that they have reduced the percentage of stearic and linoleic acids (C18) in calli than plant seed oil (Costa et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The development of biotechnological strategies oriented toward green chemistry, according to the principles of Circular Economy, is considered an alternative to boost plant oil production without compromising the food supply, in the context of the "land for food or fuel" debate, notwithstanding consumer concerns about the use of genetically modified (GM) organisms, as well as commercial and regulatory issues related to their exploitation, which mainly consider food products. Biotechnological strategies to manipulate UFAs accumulation include overexpression of enzymes of the fatty acid (FA) biosynthesis pathway, perturbation of the regulation of related biosynthetic pathways, or block of competing pathways (Bhati et al, 2021). The success of these approaches relies on a thorough mining of the key genes that determine oleic acid levels for the selection of the appropriate targets.…”

Section: Introductionmentioning

confidence: 99%

Development of a High Oleic Cardoon Cell Culture Platform by SAD Overexpression and RNAi-Mediated FAD2.2 Silencing

et al. 2022

View full text Add to dashboard Cite

The development of effective tools for the sustainable supply of phyto-ingredients and natural substances with reduced environmental footprints can help mitigate the dramatic scenario of climate change. Plant cell cultures-based biorefineries can be a technological advancement to face this challenge and offer a potentially unlimited availability of natural substances, in a standardized composition and devoid of the seasonal variability of cultivated plants. Monounsaturated (MUFA) fatty acids are attracting considerable attention as supplements for biodegradable plastics, bio-additives for the cosmetic industry, and bio-lubricants. Cardoon (Cynara cardunculus L. var. altilis) callus cultures accumulate fatty acids and polyphenols and are therefore suitable for large-scale production of biochemicals and valuable compounds, as well as biofuel precursors. With the aim of boosting their potential uses, we designed a biotechnological approach to increase oleic acid content through Agrobacterium tumefaciens-mediated metabolic engineering. Bioinformatic data mining in the C. cardunculus transcriptome allowed the selection and molecular characterization of SAD (stearic acid desaturase) and FAD2.2 (fatty acid desaturase) genes, coding for key enzymes in oleic and linoleic acid formation, as targets for metabolic engineering. A total of 22 and 27 fast-growing independent CcSAD overexpressing (OE) and CcFAD2.2 RNAi knocked out (KO) transgenic lines were obtained. Further characterization of five independent transgenic lines for each construct demonstrated that, successfully, SAD overexpression increased linoleic acid content, e.g., to 42.5%, of the relative fatty acid content, in the CcSADOE6 line compared with 30.4% in the wild type (WT), whereas FAD2.2 silencing reduced linoleic acid in favor of the accumulation of its precursor, oleic acid, e.g., to almost 57% of the relative fatty acid content in the CcFAD2.2KO2 line with respect to 17.7% in the WT. Moreover, CcSADOE6 and CcFAD2.2KO2 were also characterized by a significant increase in total polyphenolic content up to about 4.7 and 4.1 mg/g DW as compared with 2.7 mg/g DW in the WT, mainly due to the accumulation of dicaffeoyl quinic and feruloyl quinic acids. These results pose the basis for the effective creation of an engineered cardoon cells-based biorefinery accumulating high levels of valuable compounds from primary and specialized metabolism to meet the industrial demand for renewable and sustainable sources of innovative bioproducts.

show abstract

“…In recent decades, researchers have devoted significant attention to enhancing the quality and quantity of seed oils for both food and non-food purposes, as well as exploring new oil sources (Subedi et al, 2020a and b;Bhati et al, 2021;Chen et al, 2021a;Sheri et al, 2021). The fatty acid composition of plant oils plays a crucial role in determining their quality and suitability for various applications, primarily by distinguishing between saturated and unsaturated FA.…”

Section: Genetic Engineering For Improved Oil Productionmentioning

confidence: 99%

Boosting plant oil yields: the role of genetic engineering in industrial applications

Hajinajaf,

Fayyazbakhsh,

Kamal Shahsavar

et al. 2024

Biofuel Res. J.

View full text Add to dashboard Cite

As climate change intensifies and the need to reduce human-caused emissions becomes more urgent, transitioning to a bio-based economy is essential. This paper explores the diverse industrial applications of plant oils as sustainable alternatives to petroleum-based products, including their use in food, polymers, lubricants, surfactants, pesticides, emollients, and biofuels. This review delves into biosynthetic pathways, detailing the key enzymes and processes involved in the synthesis of triacylglycerol. It thoroughly discusses how genetic and metabolic engineering can not only increase oil yields but also modify fatty acid compositions to better meet industrial requirements. By understanding genetics and utilizing advanced biotechnologies, the oil content and quality of plant sources can be significantly enhanced, aligning with both sustainability goals and industrial demands. This paper provides a comprehensive overview of the current uses and genetic engineering of plant oil production, proposing innovative strategies such as utilizing oils from biomass or cultivating non-edible oil crops. These approaches aim to establish a sustainable industrial system, reduce reliance on fossil fuels, and promote the growth of an environmentally responsible bio-based economy. Additionally, the review highlights future directions, examining the economic implications and environmental benefits of adopting plant oils across various sectors and positioning them as pivotal to achieving an eco-friendly, bio-based economy.

show abstract

The Survey of Genetic Engineering Approaches for Oil/Fatty Acid Content Improvement in Oilseed Crops

Cited by 4 publications

References 92 publications

Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean

Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean

Development of a High Oleic Cardoon Cell Culture Platform by SAD Overexpression and RNAi-Mediated FAD2.2 Silencing

Boosting plant oil yields: the role of genetic engineering in industrial applications

Contact Info

Product

Resources

About