Overcoming dormancy and characterization of germination in Cerrado ‘algodãozinho’ seeds treated with dimethyl sulphoxide

Batista, Priscila Ferreira; Costa, Ana Letícia Rodrigues; Megguer, Clarice Aparecida; Lima, Júlien da Silva; Sales, Juliana de Fátima

doi:10.1016/j.sajb.2014.02.004

Cited by 5 publications

(8 citation statements)

References 15 publications

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“…Gavilan et al 2014). Roots and barks from C. regium plants are used in popular medicine to treat female reproductive system infections, intestinal inflammation, rheumatoid arthritis, ulcers, leucorrhoea, cancer and gastritis (TRESVENZOL et al, 2006;SOUZA & FELFILLI, 2006;MOREIRA & GUARIM-NETO, 2009;SÓLON et al, 2009;MELO et al, 2011;BATISTA et al, 2014). Phytochemical analyses of C. regium extract indicated the presence of essential oils, flavonoids, saponins, triacylbenzenes, tannins, phenol derivatives, steroidal triterpenes and gallic acid derivatives, providing support to justify its use as popular phytotherapic (BRUM et al, 1997;SÓLON et al, 2012).…”

Section: Biologymentioning

confidence: 99%

“…Several plants from Cerrado are known as source of medical compounds and food interests (CARAMORI et al, 2004). Cochlospermum regium (Schrank) Pilger, popularly known as 'algodãozinho' or 'algodãozinho do cerrado', is a medicinal species belonging to Bixaceae family and is commonly found in Cerrado (SÓLON et al, 2009;BATISTA et al, RESUMO: As raízes de Cochlospermum regium são usadas na medicina popular, pois seu extrato apresenta diversas moléculas fitoquímicas, algumas com atividade antimicrobiana, que, consequentemente, expõe esta espécie ao risco de erosão genética. Assim, o objetivo deste estudo foi elaborar um protocolo de multiplicação in vitro para explantes de C. regium usando esterilização química do meio de cultura.…”

Section: Introductionunclassified

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Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

et al. 2018

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Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method.

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Section: Biologymentioning

confidence: 99%

Section: Introductionunclassified

Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

et al. 2018

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“…ex Schrank) Pilg., conhecido popularmente por "algodãozinho-do-cerrado, algodoeiro-do-campo ou pacote", pertence à Família Bixaceae, subordinada à ordem Malvales, com 12 espécies de ocorrência pantropical (LLERAS, 2015;DUARTE et al, 2014). Esta espécie apresenta disposição heterogênea em áreas do domínio Cerrado brasileiro, sendo encontrado em campo sujo, Cerrado sentido restrito, em beiras de estradas e em áreas rurais antropomorfizadas, sendo considerada por Batista et al (2014), e Coelho et al (2008 uma espécie pioneira. O extrativismo e a destruição dos habitats de Cerrado colocaram C. regium na lista de espécies vegetais em risco de extinção.…”

Section: Introductionunclassified

“…O rizoma e as cascas de C. regium são amplamente estudadas (MAI et al, 2016;BATISTA et al, 2014;CUNHA-LAURA et al, 2013;MARCELINO SANTOS NETO, 2010;ANDRADE et al, 2008;TOLEDO et al, 2000), entretanto, os órgãos aéreos talos, folhas, flores, frutos e sementes apresentam escassos trabalhos fitoquímicos (DOS SANTOS et al, 2012), e inexistem trabalhos para o extrato floral e capítulos florais. Sendo necessário que estudos sejam realizados para este órgão de reprodução, avaliando quais são os constituintes químicos fitoterápicos presentes.…”

Section: Introductionunclassified

Estudo fitoquímico, bioativo, fotoprotetor e físico-químico do extrato floral de Cochlospermum regium (Schrank.) Pilg. – Bixaceae (algodãozinho do cerrado)

Filho

Santos

Castro

2021

Revista Arquivos Científicos (IMMES)

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“…Cochlospermum regium (Schrank) Pilger is a shrub of the family Bixaceae Kunth, popularly known as “algodãozinho-do-campo” or “algodãozinho-do-cerrado” [ 12 ]. According to sellers of medicinal herbs, C. regium roots and xylopods are among the most sought-after medicinal plants and are indicated for the therapeutic treatment of uterine, ovarian, and prostate inflammation and infection [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Control of Uropathogenic Microorganisms with the Ethanolic Extract from the Leaves of Cochlospermum regium (Schrank) Pilger

Leme

Rodrigues

Almeida-Apolonio

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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The roots of Cochlospermum regium, popularly known as “algodãozinho-do-cerrado,” are used for the treatment of genitourinary infections. However, the removal of their subterranean structures results in the death of the plant, and the use of the leaves becomes a viable alternative. Therefore, the antimicrobial activity of Cochlospermum regium leaf's ethanolic extract and its action on the biofilm formation of microorganisms associated with urinary infection were evaluated. The total phenolic compounds, flavoids, and tannins were quantified using the reagents Folin-Ciocalteu, aluminum chloride, and vanillin, respectively. The antimicrobial activity was evaluated by the broth microdilution method and the effect of the extract in the biofilm treatment was measured by the drop plate method. Cytotoxicity was evaluated by the method based on the reduction of MTS and the mutagenicity by the Ames test. The ethanolic extract of C. regium leaves presented 87.4 mg/EQ of flavonoids, 167.2 mg/EAG of total phenolic compounds, and 21.7 mg/ECA of condensed tannins. It presented reduction of the biofilm formation for E. coli and C. tropicalis and antimicrobial action of 1 mg/mL and 0.5 mg/mL, respectively. The extract showed no cytotoxicity and mutagenicity at the concentrations tested. This study demonstrated that C. regium leaves are a viable option for the treatment of genitourinary infections and for the species preservation.

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Overcoming dormancy and characterization of germination in Cerrado ‘algodãozinho’ seeds treated with dimethyl sulphoxide

Cited by 5 publications

References 15 publications

Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

Estudo fitoquímico, bioativo, fotoprotetor e físico-químico do extrato floral de Cochlospermum regium (Schrank.) Pilg. – Bixaceae (algodãozinho do cerrado)

In Vitro Control of Uropathogenic Microorganisms with the Ethanolic Extract from the Leaves of Cochlospermum regium (Schrank) Pilger

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