Overcoming Antibiotic Resistance in Microorganisms: Molecular Mechanisms

Gabibov, Alexander; Dontsova, Olga А.; Egorov, A. M.

doi:10.1134/s0006297920110012

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…The search for new antimicrobial agents remains a crucial and urgent task, which is largely due to the existence and endless emergence of resistant bacterial strains with various mechanisms of acquired resistance to nearly all clinically relevant antibiotics. These mechanisms include mutations in the drug target site, enzymatic modification or degradation of antibiotics, and active efflux through porins and other permeability barriers [ 1 , 2 , 3 ].…”

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confidence: 99%

“…Suppression of the growth of E. coli strains, with the deletion of the tolC gene and the harboring CHL acetyltransferase (cat) gene (E.coli ∆tolC-CAT) or B. subtilis CHL-resistant strains (B. subtilis pHT01-cat) and the harboring methyltransferase Cfr (cfr) gene (B. subtilis pHT01-cfr) by CAM-Cn-TPPs. The values of a minimal inhibitory concentration (MIC, µM) are shown1 . The MIC values were determined using the double-dilution method.…”

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confidence: 99%

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Triphenilphosphonium Analogs of Chloramphenicol as Dual-Acting Antimicrobial and Antiproliferating Agents

et al. 2021

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In the current work, in continuation of our recent research, we synthesized and studied new chimeric compounds, including the ribosome-targeting antibiotic chloramphenicol (CHL) and the membrane-penetrating cation triphenylphosphonium (TPP), which are linked by alkyl groups of different lengths. Using various biochemical assays, we showed that these CAM-Cn-TPP compounds bind to the bacterial ribosome, inhibit protein synthesis in vitro and in vivo in a way similar to that of the parent CHL, and significantly reduce membrane potential. Similar to CAM-C4-TPP, the mode of action of CAM-C10-TPP and CAM-C14-TPP in bacterial ribosomes differs from that of CHL. By simulating the dynamics of CAM-Cn-TPP complexes with bacterial ribosomes, we proposed a possible explanation for the specificity of the action of these analogs in the translation process. CAM-C10-TPP and CAM-C14-TPP more strongly inhibit the growth of the Gram-positive bacteria, as compared to CHL, and suppress some CHL-resistant bacterial strains. Thus, we have shown that TPP derivatives of CHL are dual-acting compounds targeting both the ribosomes and cellular membranes of bacteria. The TPP fragment of CAM-Cn-TPP compounds has an inhibitory effect on bacteria. Moreover, since the mitochondria of eukaryotic cells possess qualities similar to those of their prokaryotic ancestors, we demonstrate the possibility of targeting chemoresistant cancer cells with these compounds.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Triphenilphosphonium Analogs of Chloramphenicol as Dual-Acting Antimicrobial and Antiproliferating Agents

et al. 2021

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“…The formation of antibiotic resistance in bacteria is an adaptive survival mechanism that creates a global health problem [1][2][3][4][5][6]. The wide prevalence of E. coli and its ability to quickly acquire resistance to antibacterial drugs and disinfectants, including through the formation of biofilms and horizontal gene transfer, allow us to consider multidrug-resistant uropathogenic E. coli (UPEC) as a dangerous infectious agent that can cause urinary tract infections (UTIs), purulent infections, septicemia, and many other diseases [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

The Effects of L-Lysine-α-oxidase Enzyme and Trichoderma harzianum Rifai Culture Liquid on the Formation of Biofilms by Uropathogenic Multiresistant E. coli

et al. 2023

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In this study, the data of the antagonistic action of the enzyme L-lysine-α-oxidase in relation to clinical isolates of multiresistant uropathogenic Escherichia coli isolated from patients aged 2 to 17 years of various genders with an established diagnosis of infectious urethritis and/or cystitis are presented. According to the results obtained, the top priority drugs for the treatment of infectious urethritis and/or cystitis are FO (Fosfomycin, 200 µg/disc), IMP (Imipenem, 10 mg/disc), and CIP (Ciprofloxacin, 30 µg/disc). It was found that out of 70 clinical isolates, only 36 of them formed biofilms using the plate method, which is equal to 51.4% of the total number of isolates studied. Despite polyresistance, clinical isolates of E. coli have moderate sensitivity to both the homogeneous enzyme and the culture fluid of the producer. The introduction of an enzyme or culture liquid at an early stage of strain cultivation significantly inhibits the formation of biofilms (91–100%). When introduced at later stages of the experiment—24 h and 48 h—inhibition is less pronounced—20–36% and 5–22%, respectively.

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When the solution becomes the problem: a review on antimicrobial resistance in dairy cattle

Pires,

Pereira,

Fangueiro

et al. 2024

Future Microbiology

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Antibiotics' action, once a ‘magic bullet’, is now hindered by widespread microbial resistance, creating a global antimicrobial resistance (AMR) crisis. A primary driver of AMR is the selective pressure from antimicrobial use. Between 2000 and 2015, antibiotic consumption increased by 65%, reaching 34.8 billion tons, 73% of which was used in animals. In the dairy cattle sector, antibiotics are crucial for treating diseases like mastitis, posing risks to humans, animals and potentially leading to environmental contamination. To address AMR, strategies like selective dry cow therapy, alternative treatments (nanoparticles, phages) and waste management innovations are emerging. However, most solutions are in development, emphasizing the urgent need for further research to tackle AMR in dairy farms.

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Overcoming Antibiotic Resistance in Microorganisms: Molecular Mechanisms

Cited by 3 publications

References 13 publications

Triphenilphosphonium Analogs of Chloramphenicol as Dual-Acting Antimicrobial and Antiproliferating Agents

Triphenilphosphonium Analogs of Chloramphenicol as Dual-Acting Antimicrobial and Antiproliferating Agents

The Effects of L-Lysine-α-oxidase Enzyme and Trichoderma harzianum Rifai Culture Liquid on the Formation of Biofilms by Uropathogenic Multiresistant E. coli

When the solution becomes the problem: a review on antimicrobial resistance in dairy cattle

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