The Effects of L-Lysine-α-oxidase Enzyme and Trichoderma harzianum Rifai Culture Liquid on the Formation of Biofilms by Uropathogenic Multiresistant E. coli

Abstract

In this study, the data of the antagonistic action of the enzyme L-lysine-α-oxidase in relation to clinical isolates of multiresistant uropathogenic Escherichia coli isolated from patients aged 2 to 17 years of various genders with an established diagnosis of infectious urethritis and/or cystitis are presented. According to the results obtained, the top priority drugs for the treatment of infectious urethritis and/or cystitis are FO (Fosfomycin, 200 µg/disc), IMP (Imipenem, 10 mg/disc), and CIP (Ciprofloxacin, 30 µg/disc). It was found that out of 70 clinical isolates, only 36 of them formed biofilms using the plate method, which is equal to 51.4% of the total number of isolates studied. Despite polyresistance, clinical isolates of E. coli have moderate sensitivity to both the homogeneous enzyme and the culture fluid of the producer. The introduction of an enzyme or culture liquid at an early stage of strain cultivation significantly inhibits the formation of biofilms (91–100%). When introduced at later stages of the experiment—24 h and 48 h—inhibition is less pronounced—20–36% and 5–22%, respectively.

The Effects of Angelica ternata Extract from Kyrgyzstan on the Formation of Candida albicans ATCC 10231 Biofilms

“…The plate was incubated for 15 min, washed again with PB solution, and dried for 15 min. After that, 150 µL of 96% ethanol was added to each well, then incubated for 15 min and the result was recorded on the microplate reader at a wavelength of 492 nm [25,43,44]. The average decrease was measured and used to calculate the biofilm inhibition percentage by ATE, where OD AS was the optical density average of C. albicans samples in the experiment and OD AC was the optical density average of isolates in control without ATE:…”

The Influence of L-Lysine-Alpha-Oxidase on the Biofilm Formation of Opportunistic Microorganisms Associated with Inflammatory Diseases of the Urinary Tract

“…A few studies have shown its antitumor [ 20 , 21 ] and antifungal [ 15 , 22 ] activities. Previously, our study demonstrated the effect of LO produced by the fungus Trichoderma harzianum Rifai on the process of biofilm formation in UPEC [ 23 ].…”