Over-expression of ArathEULS3 confers ABA sensitivity and drought tolerance in Arabidopsis

Li, Dekuan; Wang, Xiaoyu; Yuan, Dezhi; Zhang, Liang; Jiang, Xin; Tao, Zhiwen; Liu, Ying; Wang, Jianmei; Li, Xufeng; Yang, Yi

doi:10.1007/s11240-014-0453-0

Cited by 19 publications

(22 citation statements)

References 57 publications

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“…Similar results were reported for other plant proteins such as the polyadenylated (poly(A)+) mRNA binding protein PAB-2 [7] and the VASCULAR PLANT ONE-ZINC FINGER (VOZ)1/and VOZ2 [36], which in normal conditions localize to the cytoplasm and the nucleus, and are translocated to stress granules upon stress. However, despite the fact that ArathEULS3 transcript levels are known to be upregulated in response to drought stress the ArathEULS3 protein was not identified in the proteome of heat/dark treated plants [10] nor was it identified when examining the changes in RNA-binding proteome under drought stress conditions [9,37]. This might be explained by the fact that the proteome of the stress granules might change depending on the nature and severity of the stress conditions as well as the developmental stage of the plant [1].…”

Section: Discussionmentioning

confidence: 99%

The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion

Dubiel

Coninck

Osterne

et al. 2020

IJMS

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Stress granules are cytoplasmic compartments, which serve as mRNA storage units during stress, therefore regulating translation. The Arabidopsis thaliana lectin ArathEULS3 has been widely described as a stress inducible gene. This study aimed to examine in detail the localization of ArathEULS3 lectin in normal and stressed cells. Colocalization experiments revealed that the nucleo-cytoplasmic lectin ArathEULS3 relocates to stress granules after stress. The ArathEULS3 sequence encodes a protein with a EUL lectin domain and an N-terminal domain with unknown structure and function. Bioinformatics analyses showed that the N-terminal domain sequence contains intrinsically disordered regions and likely does not exhibit a stable protein fold. Plasmolysis experiments indicated that ArathEULS3 also localizes to the apoplast, suggesting that this protein might follow an unconventional route for secretion. As part of our efforts we also investigated the interactome of ArathEULS3 and identified several putative interaction partners important for the protein translation process.

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Section: Discussionmentioning

confidence: 99%

The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion

Dubiel

Coninck

Osterne

et al. 2020

IJMS

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“…In addition, Arabidopsis plants overexpressing ArathEULS3 revealed a better tolerance to drought stress (Li et al 2014) and Pseudomonas syringae infection (Van Hove et al 2014). All these findings support the statement that EUL genes are actively involved in the signal cascades triggered by several abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

Evolutionary relationships and expression analysis of EUL domain proteins in rice (Oryza sativa)

Schutter

Tsaneva

Kulkarni

et al. 2017

Rice

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Background: Lectins, defined as 'Proteins that can recognize and bind specific carbohydrate structures', are widespread among all kingdoms of life and play an important role in various biological processes in the cell. Most plant lectins are involved in stress signaling and/or defense. The family of Euonymus-related lectins (EULs) represents a group of stress-related lectins composed of one or two EUL domains. The latter protein domain is unique in that it is ubiquitous in land plants, suggesting an important role for these proteins. Results: Despite the availability of multiple completely sequenced rice genomes, little is known on the occurrence of lectins in rice. We identified 329 putative lectin genes in the genome of Oryza sativa subsp. japonica belonging to nine out of 12 plant lectin families. In this paper, an in-depth molecular characterization of the EUL family of rice was performed. In addition, analyses of the promoter sequences and investigation of the transcript levels for these EUL genes enabled retrieval of important information related to the function and stress responsiveness of these lectins. Finally, a comparative analysis between rice cultivars and several monocot and dicot species revealed a high degree of sequence conservation within the EUL domain as well as in the domain organization of these lectins.

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“…Furthermore, Wang et al (2011) listed ArathEULS3 among the 67 most important ABA-induced genes, specifically in stomata. Arabidopsis overexpressing ArathEULS3 showed fewer disease symptoms after infection with P. syringae (Van Hove et al, 2015) and revealed increased tolerance to drought stress (Li et al, 2014). Additionally, the in-silico promoter analysis confirmed the presence of many cis-regulatory elements associated with drought/salt and ABA response in the promoter region upstream from the ArathEULS3 gene (Van Hove et al, 2014).…”

Section: Discussionmentioning

confidence: 59%

“…Plants defective in ABA biosynthesis are characterized by a larger root system and impaired inhibition of lateral root elongation. ArathEULS3 was reported to interact with the ABA receptor PYL9 (Figure 6), one of the most important players in ABA signaling (Li et al, 2014). Similar to ArathEULS3, the expression of PYL9 was most dominant in the vascular root tissues (Antoni et al, 2013).…”

Section: Discussionmentioning

confidence: 95%

Arabidopsis Lectin EULS3 Is Involved in ABA Signaling in Roots

et al. 2020

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The Arabidopsis thaliana lectin ArathEULS3 is upregulated in particular stress conditions and upon abscisic acid (ABA) treatment. ABA is a plant hormone important for plant growth and stress responses. During stress ABA is perceived by PYR/PYL/RCAR receptors, inhibiting protein phosphatases PP2Cs thereby enabling SNRK2s kinases to start downstream phosphorylation cascades and signaling. PYL9, one of the ABA receptors was identified as an interacting partner for ArathEULS3. Promoter::GUS activity studies revealed the expression of ArathEULS3 in the central root cylinder and the cells flanking young lateral root primordia, and showed enhanced expression in root tips after ABA treatment. Transcript levels for ArathEULS3 increased after exposure to ABA and osmotic treatments. ArathEULS3 CRISPR KO mutants served as a tool to expand the knowledge on the role of ArathEULS3 in plant development. KO lines revealed a longer root system compared to WT plants, and showed reduced sensitivity to ABA, salt, and osmotic conditions. Additionally it was noted that the KO mutants had more emerged lateral roots when grown in high osmotic conditions. Together these data suggest that ArathEULS3 may be an important player in ABA responses in roots.

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Over-expression of ArathEULS3 confers ABA sensitivity and drought tolerance in Arabidopsis

Cited by 19 publications

References 57 publications

The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion

The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion

Evolutionary relationships and expression analysis of EUL domain proteins in rice (Oryza sativa)

Arabidopsis Lectin EULS3 Is Involved in ABA Signaling in Roots

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