The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion

Dubiel, Malgorzata; Coninck, Tibo De; Osterne, Vinicius Jose Da Silva; Verbeke, Isabel; Damme, Daniël Van; Smagghe, Guy; Damme, Els J.M. Van

doi:10.3390/ijms21051659

Cited by 17 publications

(13 citation statements)

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“…The GUS and GFP localization assays in transgenic A. thaliana plants provided additional information about the possible function, in plants, of the AhN4L-1 protein. As previously reported for wheat germ agglutinin (WGA) (Mishkind et al, 1982;Dubiel et al, 2020a), the highly localized accumulation of both GUS and GFP signals in root tips was in concordance with the demonstrated defensive function of agglutinin-containing proteins against soil fungal pathogens (Mirelman et al, 1975). This localization also agreed with the anti-fungal effect against F. oxysporum, a notorious soil-borne pathogen of several plant crops, observed in overexpressing AhN4L-1 A. thaliana plants.…”

Section: Discussionsupporting

confidence: 89%

“…This outcome was also incompatible with the robust induction of this gene in grain amaranth plants subjected to diverse abiotic stresses, with the reported transcript accumulation of several cucumber ATT protein genes in response to cold, drought, salt, and exogenous ABA treatment (Dang et al, 2017) and with the drought-responsive nature of a Natterin-4 gene in maize (Jin et al, 2019). It was also contrary to the response and stress-triggered re-localization into stress granules of an ABA-inducible A. thaliana Euonymus europaeus-related lectin, ArathEULS3, homologous to the E. europaeus agglutinin (Fouquaert et al, 2009), also activated by drought, salt, and osmotic stresses (Van Hove et al, 2015;Dubiel et al, 2020a) and to the tolerance to salt stress observed in A. thaliana transgenic plants overexpressing a soybean agglutinin presumed to interact with the core histones H2A, H2B, and H4 (Van Holle et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Functional Characterization of an Amaranth Natterin-4-Like-1 Gene in Arabidopsis thaliana

Cabrales-Orona

Martínez‐Gallardo

Délano‐Frier

2022

Front. Sustain. Food Syst.

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The functional characterization of an Amaranthus hypochondriacus Natterin-4-Like-1 gene (AhN4L-1) coding for an unknown function protein characterized by the presence of an aerolysin-like pore-forming domain in addition to two amaranthin-like agglutinin domains is herewith described. Natterin and nattering-like proteins have been amply described in the animal kingdom. However, the role of nattering-like proteins in plants is practically unknown. The results described in this study, obtained from gene expression data in grain amaranth and from AhN4L-1-overexpressing Arabidopsis thaliana plants indicated that this gene was strongly induced by several biotic and abiotic conditions in grain amaranth, whereas data obtained from the overexpressing Arabidopsis plants further supported the defensive function of this gene, mostly against bacterial and fungal plant pathogens. GUS and GFP AhN4L-1 localization in roots tips, leaf stomata, stamens and pistils also suggested a defensive function in these organs, although its participation in flowering processes, such as self-incompatibility and abscission, is also possible. However, contrary to expectations, the overexpression of this gene negatively affected the vegetative and reproductive growth of the transgenic plants, which also showed no increased tolerance to salinity and water-deficit stress. The latter despite the maintenance of significantly higher chlorophyll levels and photosynthetic parameters under intense salinity stress. These results are discussed in the context of the physiological roles known to be played by related lectins and AB proteins in plants.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Functional Characterization of an Amaranth Natterin-4-Like-1 Gene in Arabidopsis thaliana

Cabrales-Orona

Martínez‐Gallardo

Délano‐Frier

2022

Front. Sustain. Food Syst.

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“…To approach this problem, we made use of the observation that stress granules (SGs) are formed at elevated temperatures in different plant tissues, e.g . roots, leaves and hypocotyls (Chodasiewicz et al, 2020; Dubiel et al, 2020; Hamada et al, 2018; Kosmacz et al, 2019; Modliszewski et al, 2018). It was previously shown that these SGs in Arabidopsis seedlings contain the cell cycle regulator CYCLIN-DEPENDENT KINASE A;1 (CDKA;1)(Kosmacz et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Heat stress reveals the existence of a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana

Jager-Braet

Krause

Buchholz³

et al. 2021

Preprint

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Plant growth and fertility strongly depend on environmental conditions such as temperature. Remarkably, temperature also influences meiotic recombination and thus, the current climate change will affect the genetic make-up of plants. To further understand temperature effects on meiosis, we have followed male meiocytes of Arabidopsis thaliana by live cell imaging under three different temperature regimes, at 21C and at heat shock conditions of 30C and 34C as well as after an acclimatization phase of one week at 30C. This work led to a cytological framework of meiotic progression at elevated temperature. We found that an increase to 30C, sped up meiotic progression with specific phases being more amenable to heat than others. An acclimatization phase often moderated this effect. A sudden increase to 34C promoted a faster progression of meiosis in early prophase compared to 21C. However, the phase in which cross-overs maturate was found to be delayed at 34C. Interestingly, mutants involved in the recombination pathway did not show the extension of this phase at 34C demonstrating that the delay is recombination dependent. Further analysis revealed the involvement of the ATM kinase in this prolongation indicating the existence of a specialized variant of the pachytene checkpoint in plants.

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“…The HSP70 and SnRK1 kinase have been described as stress-dependent TSN2-interactors ( Gutierrez-Beltran et al, 2020 ). Lectin ArathEULS3 interactome analysis in A. thaliana revealed the presence of translational elongation factors, ribosomal, RNA-binding, and HSPs ( Dubiel et al, 2020 ).…”

Section: Sg-associated Proteinsmentioning

confidence: 99%

Plant Stress Granules: Trends and Beyond

et al. 2021

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Stress granules (SGs) are dynamic membrane-less condensates transiently assembled through liquid–liquid phase separation (LLPS) in response to stress. SGs display a biphasic architecture constituted of core and shell phases. The core is a conserved SG fraction fundamental for its assembly and consists primarily of proteins with intrinsically disordered regions and RNA-binding domains, along with translational-related proteins. The shell fraction contains specific SG components that differ among species, cell type, and developmental stage and might include metabolic enzymes, receptors, transcription factors, untranslated mRNAs, and small molecules. SGs assembly positively correlates with stalled translation associated with stress responses playing a pivotal role during the adaptive cellular response, post-stress recovery, signaling, and metabolic rewire. After stress, SG disassembly releases mRNA and proteins to the cytoplasm to reactivate translation and reassume cell growth and development. However, under severe stress conditions or aberrant cellular behavior, SG dynamics are severely disturbed, affecting cellular homeostasis and leading to cell death in the most critical cases. The majority of research on SGs has focused on yeast and mammals as model organism. Nevertheless, the study of plant SGs has attracted attention in the last few years. Genetics studies and adapted techniques from other non-plant models, such as affinity capture coupled with multi-omics analyses, have enriched our understanding of SG composition in plants. Despite these efforts, the investigation of plant SGs is still an emerging field in plant biology research. In this review, we compile and discuss the accumulated progress of plant SGs regarding their composition, organization, dynamics, regulation, and their relation to other cytoplasmic foci. Lastly, we will explore the possible connections among the most exciting findings of SGs from mammalian, yeast, and plants, which might help provide a complete view of the biology of plant SGs in the future.

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The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion

Cited by 17 publications

References 68 publications

Functional Characterization of an Amaranth Natterin-4-Like-1 Gene in Arabidopsis thaliana

Functional Characterization of an Amaranth Natterin-4-Like-1 Gene in Arabidopsis thaliana

Heat stress reveals the existence of a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana

Plant Stress Granules: Trends and Beyond

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