Arabidopsis Lectin EULS3 Is Involved in ABA Signaling in Roots

Abstract

The Arabidopsis thaliana lectin ArathEULS3 is upregulated in particular stress conditions and upon abscisic acid (ABA) treatment. ABA is a plant hormone important for plant growth and stress responses. During stress ABA is perceived by PYR/PYL/RCAR receptors, inhibiting protein phosphatases PP2Cs thereby enabling SNRK2s kinases to start downstream phosphorylation cascades and signaling. PYL9, one of the ABA receptors was identified as an interacting partner for ArathEULS3. Promoter::GUS activity studies revealed the expression of ArathEULS3 in the central root cylinder and the cells flanking young lateral root primordia, and showed enhanced expression in root tips after ABA treatment. Transcript levels for ArathEULS3 increased after exposure to ABA and osmotic treatments. ArathEULS3 CRISPR KO mutants served as a tool to expand the knowledge on the role of ArathEULS3 in plant development. KO lines revealed a longer root system compared to WT plants, and showed reduced sensitivity to ABA, salt, and osmotic conditions. Additionally it was noted that the KO mutants had more emerged lateral roots when grown in high osmotic conditions. Together these data suggest that ArathEULS3 may be an important player in ABA responses in roots.

Overexpression of F-Box Nictaba Promotes Defense and Anthocyanin Accumulation in Arabidopsis thaliana After Pseudomonas syringae Infection

“…In the T3 generation, five homozygous knockout lines were obtained: KO1 (1 bp deletion), KO2 (1 bp insertion), KO3 (1bp deletion), KO4 (1 bp deletion), and KO5 (1 bp insertion) ( Supplementary Figure 2 ). More experimental details have been described by Dubiel et al ( 2020 ).…”

Functional Characterization of an Amaranth Natterin-4-Like-1 Gene in Arabidopsis thaliana

“…It is possible that the negative effects on plants development observed in AhN4L-1 OE transgenic A. thaliana plants were the result of fitness costs often associated with plants manipulated to have enhanced disease resistance (Bowling et al, 1994;Mauch et al, 2001) and/or due to a yet to be determined toxic effect caused by the undue accumulation of the AhN4L-1 protein. Another possible explanation of the marked root growth inhibition produced by AhN4L-1 overexpression could invoke the effects caused by a knock-out mutation of the ArathEULS3 lectin in Arabidopsis plants, which produced significantly longer primary roots, presumably as a result of reduced ABA sensitivity (Dubiel et al, 2020b). This similarity suggests that the development-related effects produced by the over expression of AhN4L-1 in Arabidopsis could have been partially due to an association with ABA signaling.…”

Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions

“…Measurement of root growth dynamics and root growth under stress conditions was conducted as described (Dubiel et al, 2020 ). In short, seeds of A. thaliana Col-0 and mutant plants were sterilized, stratified for 3 days, and grown on half-strength MS medium.…”