Ovarian follicle cell enhancers from the Drosophila yolk protein genes: different segments of one enhancer have different cell-type specificities that interact to give normal expression.

Logan, Susan K.; Wensink, Pieter C.

doi:10.1101/gad.4.4.613

Cited by 47 publications

(37 citation statements)

References 44 publications

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“…A similar, albeit simpler, arrangement of genes and enhancers in Drosophila has been found for a pair of divergently transcribed neighboring genes, ypl and yp2, which encode yolk proteins (Logan et al 1989;Logan and Wensink 1990). Transcription of this pair of genes is mediated by three enhancers, two interacting ones specific for certain lineages of ovarian follicle cells and the other for the fat bodies of adult females.…”

Section: Cold Spring Harbor Laboratory Pressmentioning

confidence: 66%

Cis-regulation of achaete and scute: shared enhancer-like elements drive their coexpression in proneural clusters of the imaginal discs.

Gómez-Skármeta¹,

Rodrı́guez²,

Martı́nez³

et al. 1995

Genes Dev.

166

105

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The pattern of bristles and other sensory organs on the adult cuticle of Drosophila is prefigured in the imaginal discs by the pattern of expression of the proneural achaete (ac) and scute (sc) genes, two members of the ac-sc complex (AS-C). These genes are simultaneously expressed by groups of cells (the proneural clusters) located at constant positions in discs. Their products {transcription factors of the basic-helix-loop-helix family) allow cells to become sensory organ mother cells (SMCs), a fate normally realized by only one or a few cells per cluster. Here we show that the highly complex pattern of proneural clusters is constructed piecemeal, by the action on ac and sc of site-specific, enhancer-like elements distributed along most of the AS-C (-90 kb). Fragments of AS-C DNA containing these enhancers drive reporter lacZ genes in only one or a few proneural clusters. This expression is independent of the ac and sc endogenous genes, indicating that the enhancers respond to local combinations of factors (prepattern). We show further that the cross-activation between ac and sc, discovered by means of transgenes containing either ac or sc promoter fragments linked to lacZ and thought to explain the almost identical patterns of ac and sc expression, does not occur detectably between the endogenous ac and sc genes in most proneural clusters. Our data indicate that coexpression is accomplished by activation of both ac and sc by the same set of position-specific enhancers.

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Section: Cold Spring Harbor Laboratory Pressmentioning

confidence: 66%

Cis-regulation of achaete and scute: shared enhancer-like elements drive their coexpression in proneural clusters of the imaginal discs.

Gómez-Skármeta¹,

Rodrı́guez²,

Martı́nez³

et al. 1995

Genes Dev.

166

105

View full text Add to dashboard Cite

show abstract

“…cases, the genes react similarly to enhancers located between them, even when, for yp-l/yp-2, the intergenic region includes multiple enhancers with different specificities (30,31).…”

Section: Resultsmentioning

confidence: 99%

A transcriptional switch between the Pig-1 and Sgs-4 genes of Drosophila melanogaster.

Mougneau¹,

Seggern²,

Fowler³

et al. 1993

Mol. Cell. Biol.

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Pig-l and Sgs-4 are a pair of closely linked and divergently transcribed Drosophila melanogaster genes, which are both expressed in larval salivary glands but at different times during development. While Sgs-4 is expressed at high levels only at the end of the third instar, Pig-i exhibits a major peak of expression during late second and early third instar. Thus, Pig-] expression declines as Sgs-4 expression is induced. In this paper, we show that three adjacent elements located within the short region between these genes can account for the switch from Pig-i to Sgs-4 expression. A 170-bp segment acts as an enhancer to direct Sgs-4 expression in late-third-instar salivary glands. A 64-bp sequence located just upstream from the enhancer can modify its temporal specificity so that it works throughout the third instar. Expression induced at mid-third instar by a combination of these two elements can be repressed by a negative regulatory sequence located still further upstream. We present evidence suggesting that the changing interactions between these regulatory elements and the Sgs-4 and Pig-i promoters lead to the correct pattern of expression of the two genes.

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“…Cis-regulatory elements were determined in the promoter regions, which are necessary for expression in ovarian follicular cells and in the fat body (Logan and Wensink, 1990;An and Wensink, 1995). Almost all studies have focused on the yp1/yp2 gene pair whose members are divergently transcribed from a common promoter region.…”

Section: Yolk Protein Gene Transcription Factorsmentioning

confidence: 99%

Vitellogenesis and Post-Vitellogenic Maturation of the Insect Ovarian Follicle

Swevers

2005

Comprehensive Molecular Insect Science

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Ovarian follicle cell enhancers from the Drosophila yolk protein genes: different segments of one enhancer have different cell-type specificities that interact to give normal expression.

Cited by 47 publications

References 44 publications

Cis-regulation of achaete and scute: shared enhancer-like elements drive their coexpression in proneural clusters of the imaginal discs.

Cis-regulation of achaete and scute: shared enhancer-like elements drive their coexpression in proneural clusters of the imaginal discs.

A transcriptional switch between the Pig-1 and Sgs-4 genes of Drosophila melanogaster.

Vitellogenesis and Post-Vitellogenic Maturation of the Insect Ovarian Follicle

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