Ovarian and uterine haemodynamics and their relation to steroid hormonal levels in postpartum Egyptian buffaloes

Abstract

The current study aimed to assess the ovarian and uterine haemodynamics in postpartum (pp) Egyptian buffaloes and their relation to steroid hormonal levels (progesterone and estrogen). Six multiparous buffalos with normal calving were examined by using trans-rectal Doppler sonography to assess the blood flow in ipsilateral and contralateral ovarian and uterine arteries. Doppler parameters viz. peak systolic velocity (PSV), blood flow volume (BFV), resistance (RI) and pulsatility index (PI) were measured at 1st till 6th postpartum weeks. In addition, diameters (mm) of the ovarian and uterine arteries and the vascularisation area (mm2) of ovary were determined. The blood samples were collected starting from 1st week post calving and every week thereafter following each ultrasound Doppler examination for assay of progesterone and estradiol. Results indicated that PI and RI increased consistently and significantly (P<0.05) till the 6th week post calving as compared to those recorded at 1st week in ipsilateral ovarian and uterine arteries. However, the blood flow volume and peak systolic velocity in both ovarian and uterine arteries decreased gradually from 1st week till the 6th week after calving. There were very strong positive correlations between PSV and BFV, in both ipsilateral and contralateral ovarian and uterine arteries. Blood concentrations of progesterone were lower (P<0.05) during the first four weeks than those recorded at 5th and 6th postpartum weeks. This trend was reversed for blood estradiol 17-β. The results of the present investigation concluded that Doppler ultrasonography is a suitable tool for assessing changes in ovarian and uterine perfusion during the puerperium.

Ovarian, uterine, and luteal vascular perfusions during follicular and luteal phases in the adult cyclic female rabbits with special orientation to their histological detection of hormone receptor

“…The higher estradiol concentration in the FP, as described in this study, may explain the increase in ovarian blood perfusion. Recent studies reported that a strong correlation exists between estradiol (E2), a potent vasodilator, and ovarian blood flow (OBF) [47,56]. Moreover, an OBF increase was noted after the treatment of postpartum ewes with estradiol 17β [57] .…”

Exogenous L‐arginine administration improves uterine vascular perfusion, uteroplacental thickness, steroid concentrations and nitric oxide levels in pregnant buffaloes under subtropical conditions

“…Concentrations of estradiol‐17 β (pg/ml; E2, EIA‐2693) and progesterone (ng/ml; P4, EIA‐1561) were analysed using DRG diagnostics (DRG, Germany, RUO in the USA) via competitive ELISA, with a sensitivity of 9.714 pg/ml, 0.045 ng/ml, respectively. The intra‐assay CVs for E2 and P4 were 5.4% and 6.81%, respectively (EL‐Sherbiny, EL‐Shahat, et al, 2022).…”

“…Uterine arteries' blood flow (UBF) controls placental functions and thickness and subsequently foetal well‐being (Elmetwally et al, 2021). Evaluation of uterine haemodynamics using Doppler ultrasonography has been considered a noninvasive, reliable and accurate tool, providing an insight into the placental and foetal status (EL‐Sherbiny, EL‐Shahat, et al, 2022).…”

Histological and hemodynamic characterization of corpus luteum throughout the luteal phase in pregnant and non-pregnant buffalos in relation to nitric oxide levels based on its anatomical determination

“…Progesterone (P4, EIA-1561) was analyzed using ELISA kits (DRG, Germany) by competitive assaying with inter and intra assays precisions of 9.96 and 5.4, respectively, and test sensitivity of 0.045 ng/ml. Serum samples were used in assaying the nitric oxide (NO) via its metabolites (NOMs; μmol/L) as previously examined in our laboratory ( 40 , 41 ). The nitric oxide inter and intra assay coefficients were 1.17% and 1.09%.…”