Outcome of Asymptomatic Infection with Rubella Virus during Pregnancy

Cradock-Watson, J. E.; Ridehalgh, Margaret K. S.; Anderson, M. C.; Pattison, J. R.

doi:10.1097/00006254-198207000-00005

Cited by 22 publications

(28 citation statements)

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“…As reinfection in pregnancy is considered currently to present minimal risk to the fetus (Morgan-Capner, 1986), distinction from asymptomatic primary rubella, which carries a significant risk to the fetus (Cradock-Watson et al 1981), is of critical importance.…”

Section: Introductionmentioning

confidence: 99%

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

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SUMMARYAn antiglobulin enzyme-linked immunosorbent assay for rubella-specific IgG1 and IgG3 was adapted to measure antibody avidity by incorporating a mild protein denaturant, diethylamine (DEA), into the serum diluent. Sera were tested at varying dilutions, both with and without DEA, if they contained sufficient specific IgG, or IgG3. The optical density (OD) was measured and curves were plotted. The highest OD (V) was noted and halved (V/2). The distance between the OD curves at V/2 was measured as the DEA shift value.Sera were examined from people whose sera contained rubella-specific antibodies as a consequence of infection or vaccination in the distant past (24 sera), recent primary rubella (66 sera), symptomatic reinfection (11 sera) or asymptomatic reinfection (64 sera). For specific IgG1 the DEA shift value was < 0W6 for cases of rubella in the distant past, compared with > 0-8 for the first month after primary infection. The maximum DEA shift value for the sera from cases of reinfection was 0-65.No serum from cases of rubella in the distant past contained sufficient specific IgG3 to estimate avidity. The sera collected within 1 month of onset of primary rubella gave DEA shift values > 0 7 compared with sera from reinfections, which gave DEA shift values < 0 6, except for two sera from a case of symptomatic reinfection.Thus the assessment of specific IgG subclass avidity is of value in differentiating serologically primary rubella from reinfection.

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Section: Introductionmentioning

confidence: 99%

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

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“…The majority had no clinical illness, but three cases had had a rubelliform illness with detectable specific IgM and two have been previously reported (MorganCapner et al 1983;. Many of the asymptomatic reinfections examined have also been reported previously (Cradock-Watson et al 1981; Morgan-Capner et al 1985). In five sera from four cases of asymptomatic reinfection rubella-specific IgM had been detected by MACRIA (all < 10 au).…”

Section: Seramentioning

confidence: 56%

“…Primary rubella in early pregnancy is of established risk to the fetus (Miller, Cradock-Watson & Pollock, 1982), whereas asymptomatic reinfection is of minimal risk (Cradock-Watson et al 1981;Morgan-Capner et al 1985). Thus there is a need to investigate other serological approaches to making the distinction.…”

Section: Introductionmentioning

confidence: 99%

Specific IgG subclass antibody in rubella virus infections

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

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SUMMARYA solid-phase antigen enzyme-linked immunosorbent assay (ELISA) was developed for the detection of rubella-specific IgG subclasses. For rubella-specific IgG, and IgG3 sera were quantitated in arbitrary units (au) by comparison with standard curves. A concentration of 3 au was taken as that indicating positivity for specific IgG1 and specific IgG3. No sera reactive for specific IgG2 and IgG4 have been found, and thus the assay reagents were controlled by testing dilutions of a standard calibrant serum containing known concentrations of the specific IgG subclasses.Of 105 unselected sera negative for rubella antibody by radial haemolysis (RH), two gave concentrations of specific IgG, > 3 au and both were positive by rubella latex agglutination (LA).

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“…Tem sido aceito que reinfecção é distinguida sorologicamente de doença subclínica pela presença de anticorpos para rubéola em período anterior à atual exposição ou pela ausência, mesmo em baixo nível, de IgM específica anti-virus da rubéola no soro coletado em ocasiões oportunas 16 .…”

Section: Resultsunclassified

Imunidade à rubéola: inquérito soro-epidemiológico em hospital, Estado de São Paulo - Brasil

1988

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Os títulos de anticorpos no soro pela reação de inibição da hemaglutinação para rubéola, empregando o caulim para adsorção de beta-lipoproteínas bloqueadores inespecíficos, foram determinados em funcionárias do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (Brasil), no período de 1982-1983 e confrontados com idade, cor, unidade hospitalar, local, cargo, tempo de emprego, antecedentes de rubéola ou comunicante na vigência ou não de gravidez. Participaram do estudo 1.886 funcionárias (88,9% de 2.121) tendo títulos com a distribuição: 9,6% <10,1,3% -10,3,5% -20, 5,8% -40,10,6% -80, 20,7% -160, 27,8% -320,12,6% -640, 7,3% -1.280 e 0,8% -2.560. Houve fraca associação entre títulos e quaisquer das variáveis de confrontação (P @ 0); 87,1% das funcionárias negaram antecedentes de rubéola e destas 73,9% tiveram títulos > 20; 57,6% negaram ser comunicantes e apresentaram títulos ³ 20; em 1,1% que referiram história de rubéola, os títulos foram £20; 97% negaram contacto com rubéola durante a gravidez. Houve somente um caso de malformação congênita após rubéola no primeiro trimestre da gravidez. Das 351 funcionárias sãs, e com títulos ³ 640, em 9,4% demonstrou-se IgM específica. Não foi notada flutuação significativa dos títulos em diferentes amostras em período de observação de até um ano. Conclui-se que a maioria das funcionárias é imune à rubéola (título > 20) independente de quaisquer parâmetros analisados; a presença de IgM específica em algumas funcionárias pode ser compatível com doença subclínica. Este inquérito foi considerado útil na orientação de funcionárias grávidas comunicantes de caso suspeito ou confirmado de rubéola, e para as não-grávidas e não-imunes a indicação da profilaxia pela vacina.

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Outcome of Asymptomatic Infection with Rubella Virus during Pregnancy

Cited by 22 publications

References 0 publications

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Specific IgG subclass antibody in rubella virus infections

Imunidade à rubéola: inquérito soro-epidemiológico em hospital, Estado de São Paulo - Brasil

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