Outbreak of Ciprofloxacin-Resistant<i>Shigella sonnei</i>Associated with Travel to Vietnam, Republic of Korea

Kim, Jin Seok; Kim, Jae Joon; Kim, Soo Jin; Jeon, Se-Eun; Seo, Ki Yeon; Choi, Jun-Kil; Kim, Nan-Ok; Hong, Seonghun; Chung, Gyung Tae; Yoo, Cheon-Kwon; Kim, Young-Taek; Cheun, Hyeng-Il; Bae, Geun-Ryang; Yeo, Yeong-Hee; Ha, G Y; Choi, Mi-Suk; Kang, Sang Koo; Kim, Junyoung

doi:10.3201/eid2107.150363

Cited by 35 publications

(23 citation statements)

References 11 publications

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“…Turnaround time for both positive and negative results has shortened by at least 24 hours, enabling earlier diagnosis of infection, and allowing earlier treatment and public health intervention where appropriate. We continue to perform reflex cultures where bacterial pathogens are detected by PCR and believe this is an important practise in order to detect current or 1 (2) emergent resistance 16 and provide phenotypic and molecular typing where epidemiological investigations are important. 17 In summary, the GP2 assay demonstrated high specificity, exceeding 99% for all pathogens, allowing adoption of molecular screening followed by targeted culture for faecal pathogens at our laboratory.…”

Section: Discussionmentioning

confidence: 99%

Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

McAuliffe¹,

Bissessor²,

Williamson

et al. 2017

Pathology

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Section: Discussionmentioning

confidence: 99%

Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

McAuliffe¹,

Bissessor²,

Williamson

et al. 2017

Pathology

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“…Shigellosis is a global human health problem and the leading cause of diarrhea, which results in about 700,000 deaths per year worldwide (1). Despite the recent public health improvements, incidence of shigellosis is still being reported regularly (2,3). The genus Shigella belongs to the Enterobacteriaceae family and consists of four subgroups according to their biochemical and serological properties: A (Shigella dysenteriae), B (Shigella flexneri), C (Shigella boydii), and D (Shigella sonnei).…”

Section: Introductionmentioning

confidence: 99%

Determination of Antibiotic Resistance Pattern and frequency of CTX-M, TEM, and SHV Β-Lactamase Encoding Genes among Shigella Isolates from Inpatients in Tehran, Iran

2019

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Background and Objectives: The emergence of extended-spectrum β-lactamase (ESBL)-producing Shigella spp. is becoming a health concern worldwide. This study aimed to investigate antibiotic resistance pattern and frequency of blaCTX-M, blaSHV, and blaTEM genes among Shigella isolates from patients in hospitals of Tehran, Iran. Methods: In this cross-sectional study, 52 non-repeated Shigella strains were isolated from hospitalized patients in Milad, Emam Khomeini and Shariati hospitals in Tehran (Iran) from November 2015 to December 2016. Bacterial identification, serotyping, and antimicrobial susceptibility testing were performed according to the standard guidelines. The blaCTX-M, blaSHV, and blaTEM resistance genes were identified using multiplex polymerase chain reaction. Results: Among 52 Shigella isolates, S. sonnei (44.2%) was the predominant species, followed by S. flexneri and S. dysenteriae (23%). Over 67% of the isolates were multidrug resistant. The highest rates of resistance were observed against cefalotin (67.3%), tetracycline (67.3%), amikacin (63.5%), trimethoprim-sulphamethoxazole (48.1), and ampicillin (42.3%). The lowest resistance rate was against ciprofloxacin (1.9%). We detected the blaTEM and blaCTX-M genes in 61.5% and 19.2% of the isolates, respectively. However, the blaSHV gene was not detected in any of the isolates. In addition, 16.4% of the isolates harbored the blaTEM and blaCTX-M genes simultaneously. Ciprofloxacin was the most effective antibiotics according to the ESBL genes distribution. Conclusion: Our findings indicate the high prevalence of multidrug resistance and ESBL genes in Shigella isolates, which elucidates the need for appropriate infection control measures for limiting the spread of resistant strains.

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“…Since the first isolation of S. sonnei with reduced susceptibility to ciprofloxacin in Japan in 1993 [11], ciprofloxacin resistant S. sonnei have been increasingly reported throughout Asia [12-14]. Furthermore, public health laboratories in several non-Asian countries with low incidences of shigellosis have reported the isolation of ciprofloxacin resistant S. sonnei , often from individuals reporting recent travel to locations with a high risk of shigellosis [15-17].…”

Section: Introductionmentioning

confidence: 99%

South Asia as a reservoir for the global spread of ciprofloxacin resistantShigella sonnei

Rabaa

Thanh

Lappe

et al. 2016

Preprint

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59Background 60 Antimicrobial resistance is a major issue in the Shigellae, particularly as a specific multidrug resistant 61 (MDR) lineage of Shigella sonnei (lineage III) is becoming globally dominant. Ciprofloxacin is a 62 recommended treatment for Shigella infections. However, ciprofloxacin resistant S. sonnei are being 63 increasingly isolated in Asia, and sporadically reported on other continents. 64 Methods and Findings 65Hypothesising that Asia is the hub for the recent international spread of ciprofloxacin resistant S. 66 sonnei, we performed whole genome sequencing on a collection of contemporaneous ciprofloxacin 67 resistant S. sonnei isolated in six countries from within and outside of Asia. We reconstructed the 68 recent evolutionary history of these organisms and combined these data with their geographical 69 location of isolation. Placing these sequences into a global phylogeny we found that all ciprofloxacin 70 resistant S. sonnei formed a single clade within a Central Asian expansion of Lineage III. Further, our 71 data show that resistance to ciprofloxacin within S. sonnei can be globally attributed to a single clonal 72 emergence event, encompassing sequential gyrA-S83L, parC-S80I and gyrA-D87G mutations. 73

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Outbreak of Ciprofloxacin-ResistantShigella sonneiAssociated with Travel to Vietnam, Republic of Korea

Cited by 35 publications

References 11 publications

Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

Determination of Antibiotic Resistance Pattern and frequency of CTX-M, TEM, and SHV Β-Lactamase Encoding Genes among Shigella Isolates from Inpatients in Tehran, Iran

South Asia as a reservoir for the global spread of ciprofloxacin resistantShigella sonnei

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