Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

McAuliffe, Gary; Bissessor, Liselle; Williamson, Deborah A.; Moore, Sharon P.; Wilson, J. M.; Dufour, Muriel; Taylor, Susan; Upton, Arlo

doi:10.1016/j.pathol.2017.02.003

Cited by 14 publications

(18 citation statements)

References 15 publications

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“…As these tests become more prevalent in clinical microbiology laboratories, it is essential that adequate evaluation of molecular tests for the diagnosis of gastrointestinal infections is undertaken. Recent evaluation studies demonstrate that the use of molecular-based diagnostics increases the sensitivity of testing for some enteric pathogens, with overall high negative predictive values [4][5][6][7][8][9][10]. However, the UK National Institute for Health and Care Excellence (NICE) published their diagnostics guidance in January 2017 [11], based on a Diagnostic Assessment Report commissioned by the National Institute of Health Research (NIHR) Health Technology Assessment (HTA) Programme [12].…”

Section: Evidence To Support Molecular-based Diagnostic Testingmentioning

confidence: 99%

“…Molecular techniques can detect nucleic acid from organisms at very low levels within samples, leading to an overall increased positivity rate [4][5][6][7][8][9][10]14]. GEMS (Global Enteric Multicentre Study) was a case-control study of diarrhoea in children <five years old in Africa and Asia, published in 2013 [15].…”

Section: Evidence To Support Molecular-based Diagnostic Testingmentioning

confidence: 99%

“…Freeman et al, (2017) performed a meta-analysis of 10 evaluation studies which each compared molecular gastrointestinal pathogen panel assays with standard microbiology testing [6]. [5,16]. In a large evaluation of the EntericBio Real-Time Gastro Panel 2, 19% of Salmonella spp.…”

Section: Limitations Associated With the Use Of Molecular-based Diagnmentioning

confidence: 99%

“…In a large evaluation of the EntericBio Real-Time Gastro Panel 2, 19% of Salmonella spp. culture-positive samples were missed [5].…”

Section: Limitations Associated With the Use Of Molecular-based Diagnmentioning

confidence: 99%

“…The introduction of molecular technologies into a clinical microbiology laboratory, such as multiplex polymerase chain reaction (PCR) assays, can benefit the diagnosis of gastrointestinal infection by facilitating simultaneous detection of pathogens (bacterial, parasitic and viral) directly from faeces. Molecular-based diagnosis gives a laboratory the potential to increase sample throughput, increase the amount of information obtained from a single test and decrease sample turnaround times [4][5][6][7][8][9][10]. Additionally, simple workflows and a reduction in the need for technical expertise are appealing attributes of these diagnostic methods.…”

Section: Introductionmentioning

confidence: 99%

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Molecular versus culture-based testing for gastrointestinal infection

Macfarlane-Smith

Ahmed

Wilcox

2018

Current Opinion in Gastroenterology

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Molecular techniques are capable of replacing culture in the diagnosis of gastrointestinal infections. Whether all positive results, however, represent true infection is still debateable, as is the clinical significance of identifying more than one pathogen. As it currently stands, microbiological culture remains vital for public health surveillance, monitoring antibiotic resistance and managing outbreaks.

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Section: Evidence To Support Molecular-based Diagnostic Testingmentioning

confidence: 99%

Section: Evidence To Support Molecular-based Diagnostic Testingmentioning

confidence: 99%

Section: Limitations Associated With the Use Of Molecular-based Diagnmentioning

confidence: 99%

“…In a large evaluation of the EntericBio Real-Time Gastro Panel 2, 19% of Salmonella spp. culture-positive samples were missed [5].…”

Section: Limitations Associated With the Use Of Molecular-based Diagnmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular versus culture-based testing for gastrointestinal infection

Macfarlane-Smith

Ahmed

Wilcox

2018

Current Opinion in Gastroenterology

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Impact of the introduction of nucleic acid amplification testing on Clostridioides difficile detection and ribotype distribution in Wales

2021

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Cryptosporidium spp. Diagnosis and Research in the 21st Century

O’Leary

Sleator

Lucey

2021

Food and Waterborne Parasitology

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The protozoan parasite Cryptosporidium has emerged as a leading cause of diarrhoeal illness worldwide, posing a significant threat to young children and immunocompromised patients. While endemic in the vast majority of developing countries, Cryptosporidium also has the potential to cause waterborne epidemics and large scale outbreaks in both developing and developed nations. Anthroponontic and zoonotic transmission routes are well defined, with the ingestion of faecally contaminated food and water supplies a common source of infection. Microscopy, the current diagnostic mainstay, is considered by many to be suboptimal. This has prompted a shift towards alternative diagnostic techniques in the advent of the molecular era. Molecular methods, particularly PCR, are gaining traction in a diagnostic capacity over microscopy in the diagnosis of cryptosporidiosis, given the laborious and often tedious nature of the latter. Until now, developments in the field of Cryptosporidium detection and research have been somewhat hampered by the intractable nature of this parasite. However, recent advances in the field have taken the tentative first steps towards bringing Cryptosporidium research into the 21 st century. Herein, we provide a review of these advances.

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Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

Cited by 14 publications

References 15 publications

Molecular versus culture-based testing for gastrointestinal infection

Molecular versus culture-based testing for gastrointestinal infection

Impact of the introduction of nucleic acid amplification testing on Clostridioides difficile detection and ribotype distribution in Wales

Cryptosporidium spp. Diagnosis and Research in the 21st Century

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