Determination of Antibiotic Resistance Pattern and frequency of CTX-M, TEM, and SHV Β-Lactamase Encoding Genes among Shigella Isolates from Inpatients in Tehran, Iran

Ghannadi, Shadi Beladi; Ghane, Maryam; Babaeekhou, Laleh

doi:10.29252/mlj.13.2.8

Cited by 4 publications

(4 citation statements)

References 32 publications

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“…In our study, we found a high rate of resistance to trimethoprim-sulfamethoxazole, cefazolin, and ampicillin in Shigella isolates. Theas resistance rates were in agreement with the reports published by Zamanlou et al [18], Nodeh Farahani et al [31], and Wang et al [32] in Iran and China, but higher than the results obtained by other researchers from China [33] to cefazolin, and Iran [34], and China [33] to trimethoprim-sulfamethoxazole.…”

Section: Discussionsupporting

confidence: 92%

Antimicrobial susceptibility patterns and molecular characterization of plasmid-mediated quinolone resistance determinants among Salmonella and Shigella spp. isolated from pediatric patients

Mirzaii

Alebouyeh

Sohrabi

et al. 2022

Preprint

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Background: Diarrhea remains a serious public health problem, which could be life- threatening in children. Salmonella and Shigella are among the main responsible agents. Fluoroquinolones are the most common antibiotics prescribed to treat these infections; however, increasing rate of resistance and its spread through plasmid- mediated quinolone resistance (PMQR) genes should be considered for medication in each country. To achieve this aim, the current study was conducted to determine the frequency of PMQR genes in Shigella and Salmonella isolates of pediatric patients with acute diarrhea. Methods: In this study, Shigella and Salmonella isolates from fresh stool samples of diarrhea patients were included from May 2017 to May 2018. All the isolates were characterized by conventional phenotypic and molecular methods. The antibiotic resistance profiles and the frequency of PMQR genes were determined by standard susceptibility and molecular test methods. Results: The highest antibiotic resistance rate among Shigella and Salmonella isolates was related to trimethoprim-sulfamethoxazole (37/40; 92.5%) and cefoxitin (5/45; 11.1%), respectively. Although qnrS, qepA, and aac (6¢)-Ib-cr genes were characterized in 32.5%, 2.5%, and 2.5% of the Shigella strains, qepA (17.7%) and qnrS (4.4%) were among the common PMQR determinants in the Salmonella isolates, respectively. The reduced susceptibility to ciprofloxacin was detected among 25% and 22.2% of PMQR-harboring strains of Shigella and Salmonella, respectively. Conclusion: Low rates of ciprofloxacin resistance and low frequency of MDR Salmonella and Shigella isolates were characterized in this study. Most of the isolates that carried PMQR determinants presented only low-level resistance and reduced susceptibility to ciprofloxacin.

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Section: Discussionsupporting

confidence: 92%

Antimicrobial susceptibility patterns and molecular characterization of plasmid-mediated quinolone resistance determinants among Salmonella and Shigella spp. isolated from pediatric patients

Mirzaii

Alebouyeh

Sohrabi

et al. 2022

Preprint

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“…32 Beladi et al reported the frequencies of TEM, CTX-M, and SHV genes in S. sonnei isolates as 60.87%, 21.74%, and 0%, and 58.3%, 16.6%, and 0% in S. flexneri isolates, respectively. 33 Hartman et al study on 459 tetracyclineresistant Shigella isolates reported the frequency of tetA and The represented numbers were the mean -SD of three independent repeats; 001, vB_SflS-ISF001; 002, vB_SsoS-ISF002; W4, Shigella sonnei W4; PTCC 1234, Shigella flexneri PTCC 1234.…”

Section: Discussionmentioning

confidence: 99%

A New Phage Cocktail Against Multidrug, ESBL-Producer Isolates of Shigella sonnei and Shigella flexneri with Highly Efficient Bacteriolytic Activity

Shahin

Bouzari

Komijani

et al. 2020

Microbial Drug Resistance

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The globally increasing incidence of antibiotic resistance in pathogenic microorganisms such as Shigella, a cause of human acute gastrointestinal infections, calls for developing effective alternatives. In this study, the antibiotic resistance pattern, extended-spectrum b-lactamase (ESBL)-production, and molecular characteristics of 70 multidrug-resistant isolates belong to the two most frequent species of Shigella genus, that is, Shigella sonnei (44 isolates) and Shigella flexneri (26 isolates) were investigated. These isolates were used to evaluate both specificity and activity of Shigella-specific bacteriophages, vB_SflS-ISF001, vB_SsoS-ISF002, and a cocktail of both. Twelve out of the 21 tested resistance genes were detected in the isolates. About 59% of S. sonnei and 46% of S. flexneri isolates were identified as ESBL producers. The bacteriophages showed a high efficiency of plating (EOP ‡0.5) in about 75% of the isolates. Moreover, the growth of >85% of the isolates was inhibited by the phage cocktail of vB_SflS-ISF001 and vB_SsoS-ISF002. The phage cocktail was effective against a wide range of ESBL-positive and -negative isolates of S. sonnei and S. flexneri. Therefore, this phage cocktail has the potential to inhibit or significantly decrease the spread of drug-resistant Shigella in humans, food chains, and water/wastewater sanitation systems.

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“…In a study by Ghannadi and colleagues, out of 52 Shigella isolates, more than 67% were MDR. The highest rates of resistance were observed for cephalothin, tetracycline, amikacin, trimethoprim-sulfamethoxazole, and ampicillin, while the lowest resistance rate was observed for ciprofloxacin (33). In this study, 36% of the examined isolates (n = 60) were MDR, and the highest resistance was observed for tetracycline and beta-lactam antibiotics (ampicillin and amoxicillin).…”

Section: Discussionmentioning

confidence: 47%

“…In their study, the bla TEM gene was found to be the most prevalent among Shigella isolates, followed by the bla CTX-M gene. The bla SHV gene was not detected in any of the isolates, indicating it was the least abundant (33). The bla SHV appears to have a low detection rate among Shigella isolates in Iran (42).…”

Section: Discussionmentioning

confidence: 84%

Phenotypic and Genotypic Antibiotic Resistance Patterns of Clinical Shigella Isolates from Tehran, Iran

Tahmasbi,

Tabatabaee Bafroee,

Pakbin

2024

Jundishapur J Microbiol

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Background: The emergence of multidrug-resistant (MDR) Shigella is a health concern in both developed and developing countries. The indiscriminate use of antibiotics without considering resistance patterns has led to the emergence of resistant strains. Objectives: This study aimed to investigate the phenotypic and genotypic patterns of antibiotic resistance and virulence gene distribution in Shigella isolates from patients in Tehran, Iran. Methods: In this cross-sectional study, 60 Shigella isolates were collected from patients referred to Milad Hospital and Pasargad Laboratory in Tehran. The isolates were gathered over four months, from February 2023 to June 2023. After biochemical and genetic confirmation of the isolates, the disk diffusion method was employed to determine antibiotic resistance patterns. The distribution of beta-lactamase genes (blaCTX-M1, blaCTX-M2, blaCTX-M8, blaSHV, blaTEM, and blaOXA) and virulence genes (ial, virF, invE, sigA, and pic) was investigated in Shigella isolates using the multiplex PCR method. SPSS statistical software version 22 was used for data analysis. Results: Thirty-six percent of isolates were found to be MDR. The highest rate of resistance was against tetracycline (90%) and ampicillin (80%). The lowest resistance rate was against azithromycin (1.9%) and amikacin (7.1%). The blaCTX-M1 and blaCTX-M8 genes were more prevalent, detected in 38.3% and 50% of the isolates, respectively. However, the blaSHV and blaCTX-M2 genes were not detected in any of the isolates. Additionally, 32% of the MDR isolates harbored both the blaCTX-M1 and blaCTX-M8 genes simultaneously. The distribution of virulence genes ial, virF, invE, sigA, and pic in the studied isolates was 28.3%, 85%, 68.3%, 81.7%, and 15%, respectively. Conclusions: The present study emphasizes the increasing trend of MDR Shigella isolates. Therefore, serious measures are needed to prevent the spread of resistant genes. Furthermore, the detection of virulence factors could help in controlling shigellosis, which is a significant global health issue.

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Determination of Antibiotic Resistance Pattern and frequency of CTX-M, TEM, and SHV Β-Lactamase Encoding Genes among Shigella Isolates from Inpatients in Tehran, Iran

Cited by 4 publications

References 32 publications

Antimicrobial susceptibility patterns and molecular characterization of plasmid-mediated quinolone resistance determinants among Salmonella and Shigella spp. isolated from pediatric patients

Antimicrobial susceptibility patterns and molecular characterization of plasmid-mediated quinolone resistance determinants among Salmonella and Shigella spp. isolated from pediatric patients

A New Phage Cocktail Against Multidrug, ESBL-Producer Isolates of Shigella sonnei and Shigella flexneri with Highly Efficient Bacteriolytic Activity

Phenotypic and Genotypic Antibiotic Resistance Patterns of Clinical Shigella Isolates from Tehran, Iran

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