Otimização da reação em cadeia pela polimerase (Semi Nested-PCR) para a detecção do herpesvírus bovino tipo 1 em fragmentos de órgãos fetais e em sêmen de bovinos naturalmente infectados

Takiuchi, Elisabete; Médici, Kerlei Cristina; Alfieri, Fernandes; Alfieri, Amauri Alcindo

doi:10.5433/1679-0359.2003v24n1p43

Cited by 8 publications

(12 citation statements)

References 11 publications

(17 reference statements)

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“…Additionally, Mweene et al (1996) were able to identify the latency sites of the virus in experimentally infected animals, as DNA was isolated and amplified from the trigeminal ganglion, ovary, lung, nasal and tracheal mucosal membranes, spleen, and leucocytes taken from prescapular and pre-crural lymphatic nodules. The presence of BoHV-1 was also detected by PCR at additional locations associated with latency, such as lymph nodes and nasal mucous membranes ( VAN ENGELENBURG et al, 1995). However, there is no report of viral DNA detection in ovarian structures of naturally infected animals, which would likely be in a latent state of infection, an experimental situation similar to that encountered in this study.…”

Section: C-c+supporting

confidence: 49%

“…The PCR technique is widely used for evaluation of gametes and reproductive structures (MAHAJAN et al, 2013). These tests have been shown to efficiently detect BoHV-1 and were successfully used in samples that are similar to those evaluated in the present study, such as bovine oocytes (BIELANSKI et al, 1997), follicular liquid (MARLEY et al, 2008b;D'ANGELO et al, 2009), blood (FUCHS et al, 1999, and ovarian tissue ( VAN ENGELENBURG et al, 1995;MWEENE et al, 1996). However, the majority of those studies were performed on experimentally infected animals or samples infected in vitro; thus, the analyzed samples differed significantly from those collected from the naturally infected animals evaluated in this study.…”

Section: C-c+mentioning

confidence: 71%

“…Among these methods, polymerase chain reaction (PCR) is considered to be fast and sensitive for detection of BoHV-1 in both male and female gametes (EDENS et al, 2003;TAKIUCHI et al, 2003TAKIUCHI et al, , 2005MARLEY et al, 2008a, b). The great advantage of this technique is that it is based on the identification of the viral genome; therefore, it does not require the presence of viable viral particles (HAYDEN et al, 1991;BÉLAK;BALLAGI-PORDÁNY, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Natural infection in ovarian structures by bovine herpesvirus 1: molecular and serological detection

et al. 2015

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In this study, the polymerase chain reaction (PCR) was used to evaluate the presence of viral DNA in ovarian tissue, in the cumulus-oocyte complex (COC), follicular liquid, and blood of animals naturally infected with bovine herpesvirus-1 (BoHV-1). The serum profile of the sampled animals was also evaluated. Samples of serum, blood, ovarian tissue, follicular liquid, and COC were collected from 147 slaughterhouse animals that were not vaccinated against BoHV-1. Contaminated or insufficient samples were disregarded. Serological tests allowed the identification of serum-positive animals with neutralizing antibodies against BoHV-1. Analysis of samples by PCR revealed the presence of viral DNA in 0.9% (1/115) of the COC samples, in 4.3% (5/117) of the ovarian tissue samples, and in 2.8% (3/108) of the blood samples. Viral DNA was not detected in any of the follicular liquid samples. In serological samples, a positivity of 83.6% (117/140) was observed for BoHV-1. All PCR-positive animals, regardless of the samples analyzed, showed positivity in the serum neutralization test for the detection of BoHV-1-specific antibodies. According to these results, a high prevalence of antibodies against BoHV-1 was detected in naturally infected animals from different herds, and the molecular tests revealed the presence of viral DNA in bovine ovarian tissue, providing evidence that this might be a site of BoHV-1 infection in naturally infected animals. Key words: Bovine herpesvirus, infectious bovine rhinotracheitis, cumulus-oocyte complex, ovarian tissue ResumoNeste trabalho foi avaliada a presença do DNA viral, por meio da Reação em Cadeia da Polimerase (PCR), no tecido ovariano, nos oócitos, líquido folicular e sangue de vacas naturalmente infectadas. Também foi avaliado o perfil sorológico dos animais amostrados. Foram coletadas amostras de soro, sangue, tecido ovariano, líquido folicular e complexo cumulus-oócitos de 147 animais abatidos em frigorífico não vacinados contra o herpesvirus bovino 1 (BoHV-1). Amostras tóxicas ou insuficientes foram descartadas. Os testes sorológicos foram realizados permitindo a identificação dos animais soropositivos para anticorpos neutralizantes contra o BoHV-1. Foram realizadas as PCRs onde foi observada a presença do DNA viral em 0,9% (1/115) dos oócitos, em 4,3% (5/117) do tecido ovariano e em 2,8% (3/108) do sangue. Em nenhuma das amostras de líquido folicular foi detectado o DNA viral. Nas amostras sorológicas observou-se 83,6% (117/140) de positividade para o BoHV-1. Dentre 1 Discente, Universidade Federal de Viçosa, UFV, Viçosa, MG, Brasil. E-mail: emiliovet2004@yahoo.com.br 2 Profs., UFV, Viçosa, MG, Brasil. E-mail: epcosta@ufv.br; asjvet@hotmail.com 3 Discentes, UFV, Viçosa, MG, Brasil. E-mail: vramendes@hotmail.com; sanelylc@hotmail.com; marcusrsantos@yahoo.com os animais positivos na PCR, independente das amostras, todos apresentavam positividade no teste de soroneutralização para detecção de anticorpos para BoHV-1. Conclui-se que em animais de diferentes rebanhos analisados f...

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Section: C-c+supporting

confidence: 49%

Section: C-c+mentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

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Natural infection in ovarian structures by bovine herpesvirus 1: molecular and serological detection

et al. 2015

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“…Na dependência do estádio gestacional essas infecções podem determinar mortalidades embrionárias, fetais ou neonatais, que se refletem como repetição de cio a intervalos regulares/ irregulares, abortamentos, natimortalidades e o nascimento de animais debilitados, com peso corporal abaixo da média da raça (ELLIS et al, 1982;McGOWAN;KIRKLAND, 1995;RUDAN et al, 1999;TAKIUCHI et al, 2005).…”

Section: Ibr Bvd E Leptospiroseunclassified

“…A prática de vacinação pode também prejudicar, ou mesmo inviabilizar, a realização do diagnóstico sorológico. Com isso, na maioria das infecções do aparelho reprodutivo o diagnóstico etiológico conclusivo somente pode ser realizado por meio de técnicas laboratoriais que possibilitem a identificação do agente etiológico ou de seus componentes como proteínas e ácido nucléico (TAKIUCHI et al, 2003;CLAUS et al, 2005;FERREIRA et al, 2005;PILZ;.…”

Section: Ibr Bvd E Leptospiroseunclassified

Falhas da reprodução na pecuária bovina de corte com ênfase para causas infecciosas

Junqueira

Alfieri

2006

Sem. Ci. Agr.

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ResumoNa pecuária bovina de corte especializada na cria de bezerros a eficiência da reprodução é um dos aspectos de maior destaque para o sucesso financeiro do investimento. Na presente revisão serão abordados os principais parâmetros utilizados para avaliar o desempenho reprodutivo de vacas de corte criadas extensivamente, bem como as principais causas infecciosas de falhas na reprodução que comprometem os custos da produção. Palavras-chave: Bovinos, reprodução, IBR, BVD, leptospirose AbstractThe reproductive efficiency in beef cattle breeding herds is one of the aspects of greater highlight for the financial success of the investment. In the present revision the main parameters used to evaluate the reproductive performance of cows in extensive management will be approached, as well as the more important infectious causes of the reproductive failures that affect the costs of the production.

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Bovine herpesvirus type 1 abortions detected by a semi-nested PCR in Brazilian cattle herds

Takiuchi

Médici

Alfieri

2005

Research in Veterinary Science

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Otimização da reação em cadeia pela polimerase (Semi Nested-PCR) para a detecção do herpesvírus bovino tipo 1 em fragmentos de órgãos fetais e em sêmen de bovinos naturalmente infectados

Cited by 8 publications

References 11 publications

Natural infection in ovarian structures by bovine herpesvirus 1: molecular and serological detection

Natural infection in ovarian structures by bovine herpesvirus 1: molecular and serological detection

Falhas da reprodução na pecuária bovina de corte com ênfase para causas infecciosas

Bovine herpesvirus type 1 abortions detected by a semi-nested PCR in Brazilian cattle herds

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