Ostreopsis cf. siamensis and Ostreopsis cf. ovata from the Atlantic Iberian Peninsula: Morphological and phylogenetic characterization

David, Helena; Laza‐Martínez, Aitor; Miguel, Irati; Orive, Emma

doi:10.1016/j.hal.2013.08.006

Cited by 50 publications

(85 citation statements)

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“…In this study, in contrast, the conserved LSU and 5.8S genes, as well as the more divergent ITS regions, were able to consistently delineate species level divergences among Ostreopsis species as already demonstrated by Sato et al (2011 and David et al (2013).…”

Section: Discussioncontrasting

confidence: 39%

“…For example, phylogeographical studies based on different single and/or concatenated ribosomal genes from the Mediterranean Sea, Atlantic Ocean, Pacific areas, Japan and New Zealand revealed that the sequences belong to welldefined clades which correspond to different species or groups Sato et al, 2011;David et al, 2013). More recently, as more isolates from Atlantic, Mediterranean Sea, and Pacific regions have been sequenced, distinct subclades have become apparent.…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial, but not rDNA, genes fail to discriminate dinoflagellate species in the genus Ostreopsis

et al. 2014

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Section: Discussioncontrasting

confidence: 39%

Section: Introductionmentioning

confidence: 99%

Mitochondrial, but not rDNA, genes fail to discriminate dinoflagellate species in the genus Ostreopsis

et al. 2014

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“…KC86 and KC84 from the Aegean Sea, which belong to the Atlantic/Mediterranean Ostreopsis spp. clade [13,34]. This is a different clade from O. cf.…”

Section: Resultsmentioning

confidence: 90%

“…ovata strain used as reference. [13,34]. Its toxin profile has been investigated for the first time, highlighting some variability in toxin profile among the six analyzed Ostreopsis sp.…”

Section: Growth Curve Of Ostreopsis Sp and Eco-toxicity Bioassaymentioning

confidence: 99%

Chemical, molecular, and eco-toxicological investigation of Ostreopsis sp. from Cyprus Island: structural insights into four new ovatoxins by LC-HRMS/MS

et al. 2015

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Blooms of benthic dinoflagellates of the genus Ostreopsis (mainly O. cf. ovata and occasionally O. cf. siamensis) represent a serious concern for humans in the Mediterranean area, due to production of palytoxin-like compounds listed among the most potent marine toxins known. In this work, six strains of Ostreopsis sp. from Cyprus Island were analyzed through an integrated approach based on molecular, chemical, and eco-toxicological methods. Cypriot Ostreopsis sp. was found to be a species distinct from O. cf. ovata and O. cf. siamensis, belonging to the Atlantic/Mediterranean Ostreopsis spp. clade. Some variability in toxin profiles emerged: three strains produced ovatoxin-a (OVTX-a), OVTX-d, OVTX-e, and isobaric palytoxin, so far found only in O. cf. ovata; the other three strains produced only new palytoxin-like compounds, which we named ovatoxin-i, ovatoxin-j1, ovatoxin-j2, and ovatoxin-k. The new ovatoxins present the same carbon skeleton as ovatoxin-a, differing primarily in an additional C2H2O2 moiety and an unsaturation in the region C49-C52. Other minor structural differences were found, including the presence of a hydroxyl group at C44 (in OVTX-j1 and OVTX-k) and the lack of a hydroxyl group in the region C53-C78 (in OVTX-i and OVTX-j1). The toxin content of the analyzed Ostreopsis sp. strains was in the range 0.06-2.8 pg cell(-1), definitely lower than that of a Ligurian O. cf. ovata strain cultured under the same conditions. Accordingly, an eco-toxicological test on Artemia salina nauplii demonstrated that Ostreopsis sp. presents a very low toxicity compared to O. cf. ovata. The whole of these data suggest that Ostreopsis sp. from Cyprus Island poses a relatively low risk to humans.

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“…Sequences of several strains O. cf. ovata isolated in 2000 in a single location at Rio de Janeiro, Brazil, branched with sequences from the Mediterranean Sea and the temperate and subtropical NE Atlantic at Madeira and Canary Islands, confirming the wide distribution of this species (Penna et al, 2005(Penna et al, , 2010David, Laza-Martínez, Miguel, & Orive, 2013). Nascimento, França, Gonçalves, and Ferreira (2012b) and Nascimento, Corrêa, Menezes, Varela, Paredes, and Morris (2012a) provided additional molecular data, and confirmed the toxicity O. cf.…”

mentioning

confidence: 59%

Morphological and molecular characterization of the toxic dinoflagellate Ostreopsis cf. ovata (Dinophyta) from Brazil (South Atlantic Ocean)

Gómez¹,

Qiu

Lopes

et al. 2017

RBT

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Ostreopsis cf. ovata is a toxic epiphytic dinoflagellate widely distributed in warm waters that often co-occur with species of the genera Coolia, Fukuyoa, Gambierdiscus and Prorocentrum. We investigated a strain isolated from the coast of Ubatuba, Brazil (Southwest Atlantic Ocean) by light and epifluorescence microscopies; we also report molecular data based on the LSU rDNA and ITS markers. Cells were 35-65 µm in the dorso-ventral diameter and 20-40 µm wide. We obtained the sequence of a ~1 900 base pair region of the rRNA gene cistron. In the LSU rDNA phylogeny, the sequences under the names O. ovata and O. cf. ovata branched into three clades. The ITS marker showed greater resolving power and the sequences of O. ovata/O. cf. ovata split into five clades. Our ITS sequence branched in a clade with sequences of strains from the Mediterranean Sea, European Atlantic coasts, subtropical NE Atlantic, other sequences from Brazil at Rio de Janeiro, and a few sequences from Japan. The cell dimensions and thecal plate arrangement were under the variability range reported in other ocean regions. (Norris, Bomber, & Balech, 1985;Quod, 1994;Faust & Morton, 1995;Faust, 1999; Accoroni et al., 2017). Other ecologically related genera such as Gambierdiscus or Coolia have increased the number of species in the last decade, due to the emergence of molecular data. In contrast, the number of species of Ostreopsis has remained static between 1999 and 2016. This situation is attributable to some confusion in the diagnostic characters for the delimitation of the known species due to plasticity in its morphology (see review in Parsons et al., 2012). Schmidt (1901) described O. siamensis based on two illustrations, elongated and round cells. Fukuyo (1981) re-described O. siamensis and ascribed it to Schmidt's round cell, and the elongated cells were described as the new species O. lenticularis (Fukuyo, 1981). Ostreopsis lenticularis was distinguished from O. siamensis by lacking body undulation and having fine pores densely scattered on the thecal plates in addition to the larger pores found on both species. Fukuyo (1981) also described O. ovata as distinguishable from the other two species by having a more ovoid shape and smaller size. These diagnostic characters are not stable and the lack of genetic data for the holotype specimens lead to the use of these names as O. cf. ovata or O. cf. siamensis until accurate morphological data and genetic sequences gathered from the type localities re-defines each species (Penna et al., 2005(Penna et al., , 2010. Sequences of several strains O. cf. ovata isolated in 2000 in a single location at Rio de Janeiro, Brazil, branched with sequences from the Mediterranean Sea and the temperate and subtropical NE Atlantic at Madeira and Canary Islands, confirming the wide distribution of this species (Penna et al., 2005(Penna et al., , 2010David, Laza-Martínez, Miguel, & Orive, 2013). Nascimento, França, Gonçalves, and Ferreira (2012b) and Nascimento, Corrêa, Menezes, Varela, Paredes, and Morris...

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Ostreopsis cf. siamensis and Ostreopsis cf. ovata from the Atlantic Iberian Peninsula: Morphological and phylogenetic characterization

Cited by 50 publications

References 31 publications

Mitochondrial, but not rDNA, genes fail to discriminate dinoflagellate species in the genus Ostreopsis

Mitochondrial, but not rDNA, genes fail to discriminate dinoflagellate species in the genus Ostreopsis

Chemical, molecular, and eco-toxicological investigation of Ostreopsis sp. from Cyprus Island: structural insights into four new ovatoxins by LC-HRMS/MS

Morphological and molecular characterization of the toxic dinoflagellate Ostreopsis cf. ovata (Dinophyta) from Brazil (South Atlantic Ocean)

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