Mitochondrial, but not rDNA, genes fail to discriminate dinoflagellate species in the genus Ostreopsis

Penna, Antonella; Battocchi, Cecilia; Capellacci, Samuela; Fraga, Santiago; Aligizaki, Katerina; Lemée, Rodolphe; Vernesi, Cristiano

doi:10.1016/j.hal.2014.10.004

Cited by 31 publications

(31 citation statements)

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“…KC86 and KC84 from the Aegean Sea, which belong to the Atlantic/Mediterranean Ostreopsis spp. clade [13,34]. This is a different clade from O. cf.…”

Section: Resultsmentioning

confidence: 89%

“…ovata strain used as reference. [13,34]. Its toxin profile has been investigated for the first time, highlighting some variability in toxin profile among the six analyzed Ostreopsis sp.…”

Section: Growth Curve Of Ostreopsis Sp and Eco-toxicity Bioassaymentioning

confidence: 99%

“…Due to the variability of cell shape and size found within the different Ostreopsis species, taxonomical classification of the species based only on morphological features is not always feasible. The use of molecular methods is thus necessary, although even such methods in some cases fail to discriminate taxonomical species within the genus Ostreopsis [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Chemical, molecular, and eco-toxicological investigation of Ostreopsis sp. from Cyprus Island: structural insights into four new ovatoxins by LC-HRMS/MS

et al. 2015

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Blooms of benthic dinoflagellates of the genus Ostreopsis (mainly O. cf. ovata and occasionally O. cf. siamensis) represent a serious concern for humans in the Mediterranean area, due to production of palytoxin-like compounds listed among the most potent marine toxins known. In this work, six strains of Ostreopsis sp. from Cyprus Island were analyzed through an integrated approach based on molecular, chemical, and eco-toxicological methods. Cypriot Ostreopsis sp. was found to be a species distinct from O. cf. ovata and O. cf. siamensis, belonging to the Atlantic/Mediterranean Ostreopsis spp. clade. Some variability in toxin profiles emerged: three strains produced ovatoxin-a (OVTX-a), OVTX-d, OVTX-e, and isobaric palytoxin, so far found only in O. cf. ovata; the other three strains produced only new palytoxin-like compounds, which we named ovatoxin-i, ovatoxin-j1, ovatoxin-j2, and ovatoxin-k. The new ovatoxins present the same carbon skeleton as ovatoxin-a, differing primarily in an additional C2H2O2 moiety and an unsaturation in the region C49-C52. Other minor structural differences were found, including the presence of a hydroxyl group at C44 (in OVTX-j1 and OVTX-k) and the lack of a hydroxyl group in the region C53-C78 (in OVTX-i and OVTX-j1). The toxin content of the analyzed Ostreopsis sp. strains was in the range 0.06-2.8 pg cell(-1), definitely lower than that of a Ligurian O. cf. ovata strain cultured under the same conditions. Accordingly, an eco-toxicological test on Artemia salina nauplii demonstrated that Ostreopsis sp. presents a very low toxicity compared to O. cf. ovata. The whole of these data suggest that Ostreopsis sp. from Cyprus Island poses a relatively low risk to humans.

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“…KC86 and KC84 from the Aegean Sea, which belong to the Atlantic/Mediterranean Ostreopsis spp. clade [13,34]. This is a different clade from O. cf.…”

Section: Resultsmentioning

confidence: 89%

“…ovata strain used as reference. [13,34]. Its toxin profile has been investigated for the first time, highlighting some variability in toxin profile among the six analyzed Ostreopsis sp.…”

Section: Growth Curve Of Ostreopsis Sp and Eco-toxicity Bioassaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical, molecular, and eco-toxicological investigation of Ostreopsis sp. from Cyprus Island: structural insights into four new ovatoxins by LC-HRMS/MS

et al. 2015

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“…Amplified PCR fragments were purified (Penna et al. ) and sent to Eurofins Genomics (Ebersberg, Germany). All nucleotide sequences of ITS‐5.8S rDNA and LSU deposited in ENA‐EMBL are listed in Table .…”

Section: Methodsmentioning

confidence: 99%

“…Lately, several phylogenetic studies based on LSU and internal transcribed spacer (ITS) analyses have been carried out on Ostreopsis species, supplying an increasing number of molecular clades (and subclades) that may represent cryptic species (Penna et al. , , Sato et al. , Tawong et al.…”

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confidence: 99%

Ostreopsis fattorussoi sp. nov. (Dinophyceae), a new benthic toxic Ostreopsis species from the eastern Mediterranean Sea

Accoroni

Romagnoli

Penna

et al. 2016

Journal of Phycology

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The new benthic toxic dinoflagellate, Ostreopsis fattorussoi sp. nov., is described from the Eastern Mediterranean Sea, Lebanon and Cyprus coasts, and is supported by morphological and molecular data. The plate formula, Po, 3', 7″, 6c, 7s, 5‴, 2'''', is typical for the Ostreopsis genus. It differs from all other Ostreopsis species in that (i) the curved suture between plates 1' and 3' makes them approximately hexagonal, (ii) the 1' plate lies in the left half of the epitheca and is obliquely orientated leading to a characteristic shape of plate 6″. The round thecal pores are bigger than the other two Mediterranean species (O. cf. ovata and O. cf. siamensis). O. fattorussoi is among the smallest species of the genus (DV: 60.07 ± 5.63 μm, AP: 25.66 ± 2.97 μm, W: 39.81 ± 5.05 μm) along with O. ovata. Phylogenetic analyses based on the LSU and internal transcribed spacer rDNA shows that O. fattorussoi belongs to the Atlantic/Mediterranean Ostreopsis spp. clade separated from the other Ostreopsis species. Ostreopsis fattorussoi produces OVTX-a and structural isomers OVTX-d and -e, O. cf. ovata is the only other species of this genus known to produce these toxins. The Lebanese O. fattorussoi did not produce the new palytoxin-like compounds (ovatoxin-i, ovatoxin-j , ovatoxin-j , and ovatoxin-k) that were previously found in O. fattorussoi from Cyprus. The toxin content was in the range of 0.28-0.94 pg · cell . On the Lebanon coast, O. fattorussoi was recorded throughout the year 2015 (temperature range 18°C-31.5°C), with peaks in June and August.

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Key Questions and Recent Research Advances on Harmful Algal Blooms in Benthic Systems

et al. 2018

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Mitochondrial, but not rDNA, genes fail to discriminate dinoflagellate species in the genus Ostreopsis

Cited by 31 publications

References 58 publications

Chemical, molecular, and eco-toxicological investigation of Ostreopsis sp. from Cyprus Island: structural insights into four new ovatoxins by LC-HRMS/MS

Chemical, molecular, and eco-toxicological investigation of Ostreopsis sp. from Cyprus Island: structural insights into four new ovatoxins by LC-HRMS/MS

Ostreopsis fattorussoi sp. nov. (Dinophyceae), a new benthic toxic Ostreopsis species from the eastern Mediterranean Sea

Key Questions and Recent Research Advances on Harmful Algal Blooms in Benthic Systems

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